Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/10718 |
Resumo: | Many organic compounds present in bodies of water, called emerging contaminants, are not yet contemplated in Brazilian environmental legislation. Such compounds need to be evaluated for their high toxicity to living organisms, causing serious problems for ecosystems, animal and human health. Among these compounds, widely used, is triclosan (TCS). The increasing use of TCS by the industry and a variety of common household products, including detergents, toothpastes, soaps, among others, results in the release of large and increasing amounts of this compound in water bodies, which can affect the health and survival of organisms aquatic Brazilian legislation has established a maximum TCS concentration of 0.3% for personal care products, cosmetics and perfumes, but there is no established limit for maximum permissible concentrations in the different water bodies. Despite the concern with such contaminant, few works are addressing its physiological effects on aquatic organisms, particularly fish. The present study evaluated the effect of TCS on cardiorespiratory function in vivo and on the expression of cardiac proteins involved in the management of cardiac calcium of Brycin amazonicus. Among the main findings, the present study showed that: a) in the presence of TCS the water does not reach 100% saturation with O2; b) TCS not only reduces the metabolic rate but also increases the critical O2 uptake, reducing the ability to keep O2 uptake constant and, consequently, reducing the chances of survival in hypoxic environments; c) the ventilatory requirement is significantly increased by the TCS, showing a significant decrease in the efficiency of the gills in the exchange of O2 besides increasing the energy expenditure for the gas exchange; d) TCS had a depressant effect on heart rate and increased expression of L-type calcium channels and Na + / Ca 2+ exchanger. Together, the results obtained in the present study show that the TCS, even in low concentrations and in short exposures (96h), decreases respiratory capacity of the species and interferes in the management of cardiac calcium. The depressant effect of cardio-respiratory function in vivo as well as changes in the expression of proteins involved in the cardiac excitation-contraction coupling of matrinxã reinforce the need not only for reassessment of the use of SCC in consumer products but also for the establishment of maximum limits waters for human consumption and conservation of aquatic life. |
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Martins, Nícolas FernandesKalinin, Ana Lúciahttp://lattes.cnpq.br/5493936744279888McKenzie, David Johnhttp://lattes.cnpq.br/0281731956436645http://lattes.cnpq.br/296919278484656604f04c58-617d-4eac-b917-9e825f4af24e2018-11-27T17:59:50Z2018-11-27T17:59:50Z2018-10-26MARTINS, Nícolas Fernandes. Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10718.https://repositorio.ufscar.br/handle/ufscar/10718Many organic compounds present in bodies of water, called emerging contaminants, are not yet contemplated in Brazilian environmental legislation. Such compounds need to be evaluated for their high toxicity to living organisms, causing serious problems for ecosystems, animal and human health. Among these compounds, widely used, is triclosan (TCS). The increasing use of TCS by the industry and a variety of common household products, including detergents, toothpastes, soaps, among others, results in the release of large and increasing amounts of this compound in water bodies, which can affect the health and survival of organisms aquatic Brazilian legislation has established a maximum TCS concentration of 0.3% for personal care products, cosmetics and perfumes, but there is no established limit for maximum permissible concentrations in the different water bodies. Despite the concern with such contaminant, few works are addressing its physiological effects on aquatic organisms, particularly fish. The present study evaluated the effect of TCS on cardiorespiratory function in vivo and on the expression of cardiac proteins involved in the management of cardiac calcium of Brycin amazonicus. Among the main findings, the present study showed that: a) in the presence of TCS the water does not reach 100% saturation with O2; b) TCS not only reduces the metabolic rate but also increases the critical O2 uptake, reducing the ability to keep O2 uptake constant and, consequently, reducing the chances of survival in hypoxic environments; c) the ventilatory requirement is significantly increased by the TCS, showing a significant decrease in the efficiency of the gills in the exchange of O2 besides increasing the energy expenditure for the gas exchange; d) TCS had a depressant effect on heart rate and increased expression of L-type calcium channels and Na + / Ca 2+ exchanger. Together, the results obtained in the present study show that the TCS, even in low concentrations and in short exposures (96h), decreases respiratory capacity of the species and interferes in the management of cardiac calcium. The depressant effect of cardio-respiratory function in vivo as well as changes in the expression of proteins involved in the cardiac excitation-contraction coupling of matrinxã reinforce the need not only for reassessment of the use of SCC in consumer products but also for the establishment of maximum limits waters for human consumption and conservation of aquatic life.Muitos compostos orgânicos presentes nos corpos d’água, chamados contaminantes emergentes, ainda não são contemplados nas legislações ambientais brasileiras. Tais compostos necessitam ser avaliados por apresentarem elevada toxicidade aos organismos vivos, provocando sérios problemas aos ecossistemas e à saúde animal e humana, entre eles o triclosan (TCS). O crescente uso do TCS pela indústria e numa variedade de produtos domésticos comuns, incluindo detergentes, cremes dentais, sabonetes, entre outros, resulta em lançamento de grandes e crescentes quantidades deste composto nos corpos d’água, podendo afetar a saúde e sobrevivência de organismos aquáticos. A legislação brasileira estabeleceu uma concentração máxima de TCS de 0,3 %, para produtos de higiene pessoal, cosméticos e perfumes, mas não há nenhum limite estabelecido para concentrações máximas permitidas nos diferentes corpos d’água. Apesar da preocupação com tal contaminante, poucos são os trabalhos abordando seus efeitos fisiológicos sobre os organismos aquáticos, particularmente os peixes. O presente trabalho avaliou o efeito do TCS sobre a função cardiorrespiratória in vivo e sobre a expressão de proteínas cardíacas envolvidas no manejo de cálcio cardíaco do matrinxã, Brycon amazonicus. Entre os principais achados, o presente trabalho mostrou que: a) em presença de TCS a água não atinge 100% de saturação com O2; b) O TCS não apenas reduz a taxa metabólica como também aumenta a tenção crítica de O2, reduzindo a capacidade de manter a tomada de O2 constante e, consequentemente, reduzindo as chances de sobrevivência em ambientes hipóxicos; c) a necessidade ventilatória é significativamente aumentada pelo TCS, mostrando diminuição significativa da eficiência das brânquias na troca de O2 além de aumentar o gasto energético para a troca gasosa; d) o TCS teve um efeito depressor na frequência cardíaca e aumentou a expressão de canais de cálcio do tipo L e do trocador Na+/Ca2+. Em conjunto, os resultados obtidos no presente trabalho mostram que o TCS, mesmo em baixas concentrações e em exposições de curta duração (96h) diminui a capacidade respiratória da espécie e interfere no manejo de cálcio cardíaco. O efeito depressor da função cardio-respiratória in vivo, bem como as alterações na expressão de proteínas envolvidas no acoplamento excitação-contração cardíaco de matrinxã reforçam a necessidade não apenas de reavaliação da utilização de TCS em produtos de consumo como também do estabelecimento de limites máximos permitidos em águas para consumo humano e conservação da vida aquática.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: 1460418porUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarTriclosanMatrinxã (Peixe)Respostas CardiorespiratóriasHipóxiaCardiorespiratory responsesHypoxiaCIENCIAS BIOLOGICAS::ECOLOGIACIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradualEffects of the emerging triclosan contaminant on the matrinxan cardiorespiratory function, Brycon amazonicus, in normoxy and during gradual hippoxiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisOnlineaafde173-f4e7-439f-8655-5cfea35b64bbinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALNícolas Fernandes Martins 2018.pdfNícolas Fernandes Martins 2018.pdfapplication/pdf1649660https://repositorio.ufscar.br/bitstream/ufscar/10718/1/N%c3%adcolas%20Fernandes%20Martins%202018.pdfd720d43c672e073aa6a13d03e7312254MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
dc.title.alternative.eng.fl_str_mv |
Effects of the emerging triclosan contaminant on the matrinxan cardiorespiratory function, Brycon amazonicus, in normoxy and during gradual hippoxia |
title |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
spellingShingle |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual Martins, Nícolas Fernandes Triclosan Matrinxã (Peixe) Respostas Cardiorespiratórias Hipóxia Cardiorespiratory responses Hypoxia CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
title_full |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
title_fullStr |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
title_full_unstemmed |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
title_sort |
Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual |
author |
Martins, Nícolas Fernandes |
author_facet |
Martins, Nícolas Fernandes |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/2969192784846566 |
dc.contributor.author.fl_str_mv |
Martins, Nícolas Fernandes |
dc.contributor.advisor1.fl_str_mv |
Kalinin, Ana Lúcia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5493936744279888 |
dc.contributor.advisor-co1.fl_str_mv |
McKenzie, David John |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0281731956436645 |
dc.contributor.authorID.fl_str_mv |
04f04c58-617d-4eac-b917-9e825f4af24e |
contributor_str_mv |
Kalinin, Ana Lúcia McKenzie, David John |
dc.subject.por.fl_str_mv |
Triclosan Matrinxã (Peixe) Respostas Cardiorespiratórias Hipóxia |
topic |
Triclosan Matrinxã (Peixe) Respostas Cardiorespiratórias Hipóxia Cardiorespiratory responses Hypoxia CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Cardiorespiratory responses Hypoxia |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ECOLOGIA CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
Many organic compounds present in bodies of water, called emerging contaminants, are not yet contemplated in Brazilian environmental legislation. Such compounds need to be evaluated for their high toxicity to living organisms, causing serious problems for ecosystems, animal and human health. Among these compounds, widely used, is triclosan (TCS). The increasing use of TCS by the industry and a variety of common household products, including detergents, toothpastes, soaps, among others, results in the release of large and increasing amounts of this compound in water bodies, which can affect the health and survival of organisms aquatic Brazilian legislation has established a maximum TCS concentration of 0.3% for personal care products, cosmetics and perfumes, but there is no established limit for maximum permissible concentrations in the different water bodies. Despite the concern with such contaminant, few works are addressing its physiological effects on aquatic organisms, particularly fish. The present study evaluated the effect of TCS on cardiorespiratory function in vivo and on the expression of cardiac proteins involved in the management of cardiac calcium of Brycin amazonicus. Among the main findings, the present study showed that: a) in the presence of TCS the water does not reach 100% saturation with O2; b) TCS not only reduces the metabolic rate but also increases the critical O2 uptake, reducing the ability to keep O2 uptake constant and, consequently, reducing the chances of survival in hypoxic environments; c) the ventilatory requirement is significantly increased by the TCS, showing a significant decrease in the efficiency of the gills in the exchange of O2 besides increasing the energy expenditure for the gas exchange; d) TCS had a depressant effect on heart rate and increased expression of L-type calcium channels and Na + / Ca 2+ exchanger. Together, the results obtained in the present study show that the TCS, even in low concentrations and in short exposures (96h), decreases respiratory capacity of the species and interferes in the management of cardiac calcium. The depressant effect of cardio-respiratory function in vivo as well as changes in the expression of proteins involved in the cardiac excitation-contraction coupling of matrinxã reinforce the need not only for reassessment of the use of SCC in consumer products but also for the establishment of maximum limits waters for human consumption and conservation of aquatic life. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-27T17:59:50Z |
dc.date.available.fl_str_mv |
2018-11-27T17:59:50Z |
dc.date.issued.fl_str_mv |
2018-10-26 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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dc.identifier.citation.fl_str_mv |
MARTINS, Nícolas Fernandes. Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10718. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/10718 |
identifier_str_mv |
MARTINS, Nícolas Fernandes. Efeitos do contaminante emergente triclosan sobre a função cardiorespiratória do matrinxã, Brycon amazonicus, em normóxia e durante hipóxia gradual. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/10718. |
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https://repositorio.ufscar.br/handle/ufscar/10718 |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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