Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/13430 |
Resumo: | In order to synthetize gold nanoparticles utilizing a green process, this work intended to produce nanoparticles using bacterial crude extract. Paenibacillus polymyxa (PP) and P. terrae (PT) have biotechnological interest in medical field, and some studies have been showing the potential of endophytic microorganisms in the production of antitumoral and antibacterial substances. Nanoparticles biosynthetic techniques are no toxic and clean when compared to traditional ones, where there is intensive energy consumption and sometimes may involve toxic chemical substances. Metallic nanoparticles have important applications in medical field, specially the gold ones. Through the nanoparticles biosynthesis using P. polymyxa and P. terrae, the process was optimized considering the synthesis time, the extract concentration, the wavelength and the particle size. The experiment was divided into phases where the best focus (1, 1: 2, 1: 4, 1: 8 and 1:16) was analyzed, observing through UV-Vis spectroscopy (without visible ultraviolate) at 540 nm the best absorption of light (how much) in relation to time and over 72 hours in a 12-hour interval. As the best indicators were 1: 2 and 1: 4, for both phyto-bacterial extracts and 12 to 24 hours as the best periods. The next phase was analyzed hourly for 24 hours, with the values 1: 2 and 1: 4, for (in relation to) light absorption at 540 nm and in the spectral range of 300 to 800 nm, demonstrating a statistic of gold nanoparticle for 1: 2 dilution, whose peak at around 540 nm, and as of 7 am are already described. Based on the results of the characterization by DLS (Dynamic Light Scattering - Dynamic Light Scattering) and absorbance, such as gold nanoparticles synthesized through crude extractions PP 1: 2 and PT 1: 2 were as obtaining better results, obtaining the average level of 262 nm and 372 nm, respectively, in monodispersed systems, with the following analyzes (occurrences) after 7 hours of biosynthesis reaction, obtaining greater results between 12 and 24 hours. The main absorption was detected at the red spectral range. |
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Carvalho, Carina MirandaKurachi, Cristinahttp://lattes.cnpq.br/0194007981724312http://lattes.cnpq.br/0965679818809044ad5e6f38-aac6-463a-ad9c-33b65caf71892020-11-12T17:00:39Z2020-11-12T17:00:39Z2020-03-26CARVALHO, Carina Miranda. Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae. 2020. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13430.https://repositorio.ufscar.br/handle/ufscar/13430In order to synthetize gold nanoparticles utilizing a green process, this work intended to produce nanoparticles using bacterial crude extract. Paenibacillus polymyxa (PP) and P. terrae (PT) have biotechnological interest in medical field, and some studies have been showing the potential of endophytic microorganisms in the production of antitumoral and antibacterial substances. Nanoparticles biosynthetic techniques are no toxic and clean when compared to traditional ones, where there is intensive energy consumption and sometimes may involve toxic chemical substances. Metallic nanoparticles have important applications in medical field, specially the gold ones. Through the nanoparticles biosynthesis using P. polymyxa and P. terrae, the process was optimized considering the synthesis time, the extract concentration, the wavelength and the particle size. The experiment was divided into phases where the best focus (1, 1: 2, 1: 4, 1: 8 and 1:16) was analyzed, observing through UV-Vis spectroscopy (without visible ultraviolate) at 540 nm the best absorption of light (how much) in relation to time and over 72 hours in a 12-hour interval. As the best indicators were 1: 2 and 1: 4, for both phyto-bacterial extracts and 12 to 24 hours as the best periods. The next phase was analyzed hourly for 24 hours, with the values 1: 2 and 1: 4, for (in relation to) light absorption at 540 nm and in the spectral range of 300 to 800 nm, demonstrating a statistic of gold nanoparticle for 1: 2 dilution, whose peak at around 540 nm, and as of 7 am are already described. Based on the results of the characterization by DLS (Dynamic Light Scattering - Dynamic Light Scattering) and absorbance, such as gold nanoparticles synthesized through crude extractions PP 1: 2 and PT 1: 2 were as obtaining better results, obtaining the average level of 262 nm and 372 nm, respectively, in monodispersed systems, with the following analyzes (occurrences) after 7 hours of biosynthesis reaction, obtaining greater results between 12 and 24 hours. The main absorption was detected at the red spectral range.Para sintetizar nanopartículas de ouro através de um processo ecologicamente correto, este trabalho visou à produção de nanopartículas à base de extratos brutos produzidos por fitobactérias. As fitobactérias utilizadas possuem interesse biotecnológico na área médica, (e) estudos têm apontado o potencial dos microrganismos endofíticos na produção de substâncias antibacterianas e antitumorais. As técnicas de biossíntese de nanopartículas são atóxicas e limpas, ao contrário das sintetizadas quimicamente ou fisicamente, onde há consumo intensivo de energia e às vezes podem envolver substâncias químicas tóxicas. Foram utilizadas as nanopartículas metálicas pois possuem aplicações importantes na área médica, particularmente a nanopartícula de ouro. Através do método de biossíntese de nanopartículas foram sintetizadas as nanopartículas de ouro empregando P. polymyxa e P. terrae, e o processo foi otimizado, considerando o tempo de síntese, a concentração do extrato, o comprimento de onda e o diâmetro médio da partícula. O experimento foi dividido em fases onde foi analisado a melhor concentração (1, 1:2, 1:4, 1:8 e 1:16), observando através da espectroscopia UV-Vis (no ultraviolata visível) em 540 nm a melhor absorção da luz (quanto) em relação ao tempo ao longo de 72 horas em intervalo de 12 horas. As melhores concentrações foram de 1:2 e 1:4, para ambos os extratos das fitobactérias e de 12 a 24 horas como melhores períodos. A próxima fase foi analisada de hora em hora por 24 horas, com as concentrações 1:2 e 1:4, para a (em relação a) absorção de luz em 540 nm e na faixa espectral de 300 a 800 nm, demonstrando a síntese da nanopartícula de ouro para a diluição 1:2, cujo pico em torno de 540 nm, e a partir das 7 horas já há síntese. Baseado nos resultados da caracterização por DLS (Dynamic Light Scattering - Espalhamento Dinâmico de Luz) e absorbância, as nanopartículas de ouro sintetizadas através dos extratos brutos PP 1:2 e PT 1:2 foram as que obtiveram melhores resultados, obtendo diâmetro médio de 262 nm e 372 nm respectivamente em sistemas monodispersos, com as sínteses ocorrendo (ocorrem) a partir das 7 horas de reação de biossíntese, obtendo maior resultado entre 12 e 24 horas, e com absorção principal na região do vermelho do espectro eletromagnético.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq: 134048/2018-0porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessFitobactériaNanopartículas metálicasSíntese verdeCerradoPhyto-bacteriaMetallic nanoparticlesGreen synthesisCIENCIAS BIOLOGICAS::MICROBIOLOGIABiossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terraeBiosynthesis of gold nanoparticles by Paenibacillus polymyxa and Paenibacillus terraeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis600600cd061f7d-8718-444f-8579-b624a06801adreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertação Carina MC.pdfDissertação Carina MC.pdfDissertação de mestradoapplication/pdf1860589https://repositorio.ufscar.br/bitstream/ufscar/13430/1/Disserta%c3%a7%c3%a3o%20Carina%20MC.pdf31aebd02f4871d0feeb350aa7426f417MD51CartaOrientador.pdfCartaOrientador.pdfCarta do orientadorapplication/pdf144750https://repositorio.ufscar.br/bitstream/ufscar/13430/2/CartaOrientador.pdf9dc3460827bcbb92501c0d1a40a57f42MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/13430/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertação Carina MC.pdf.txtDissertação Carina MC.pdf.txtExtracted texttext/plain89038https://repositorio.ufscar.br/bitstream/ufscar/13430/4/Disserta%c3%a7%c3%a3o%20Carina%20MC.pdf.txt1c997e30c34218f57fc06f1c7dd3dbaaMD54CartaOrientador.pdf.txtCartaOrientador.pdf.txtExtracted texttext/plain1239https://repositorio.ufscar.br/bitstream/ufscar/13430/6/CartaOrientador.pdf.txteefd03df66985769dca48131951341b0MD56THUMBNAILDissertação Carina MC.pdf.jpgDissertação Carina MC.pdf.jpgIM Thumbnailimage/jpeg5609https://repositorio.ufscar.br/bitstream/ufscar/13430/5/Disserta%c3%a7%c3%a3o%20Carina%20MC.pdf.jpg4369608a9b68ef052d0ce5c631cbeb48MD55CartaOrientador.pdf.jpgCartaOrientador.pdf.jpgIM Thumbnailimage/jpeg5495https://repositorio.ufscar.br/bitstream/ufscar/13430/7/CartaOrientador.pdf.jpg347a97634dba31efd56df10cc361ff30MD57ufscar/134302023-09-18 18:32:03.771oai:repositorio.ufscar.br:ufscar/13430Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:03Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
dc.title.alternative.eng.fl_str_mv |
Biosynthesis of gold nanoparticles by Paenibacillus polymyxa and Paenibacillus terrae |
title |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
spellingShingle |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae Carvalho, Carina Miranda Fitobactéria Nanopartículas metálicas Síntese verde Cerrado Phyto-bacteria Metallic nanoparticles Green synthesis CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
title_short |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
title_full |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
title_fullStr |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
title_full_unstemmed |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
title_sort |
Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae |
author |
Carvalho, Carina Miranda |
author_facet |
Carvalho, Carina Miranda |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/0965679818809044 |
dc.contributor.author.fl_str_mv |
Carvalho, Carina Miranda |
dc.contributor.advisor1.fl_str_mv |
Kurachi, Cristina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0194007981724312 |
dc.contributor.authorID.fl_str_mv |
ad5e6f38-aac6-463a-ad9c-33b65caf7189 |
contributor_str_mv |
Kurachi, Cristina |
dc.subject.por.fl_str_mv |
Fitobactéria Nanopartículas metálicas Síntese verde Cerrado |
topic |
Fitobactéria Nanopartículas metálicas Síntese verde Cerrado Phyto-bacteria Metallic nanoparticles Green synthesis CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
dc.subject.eng.fl_str_mv |
Phyto-bacteria Metallic nanoparticles Green synthesis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::MICROBIOLOGIA |
description |
In order to synthetize gold nanoparticles utilizing a green process, this work intended to produce nanoparticles using bacterial crude extract. Paenibacillus polymyxa (PP) and P. terrae (PT) have biotechnological interest in medical field, and some studies have been showing the potential of endophytic microorganisms in the production of antitumoral and antibacterial substances. Nanoparticles biosynthetic techniques are no toxic and clean when compared to traditional ones, where there is intensive energy consumption and sometimes may involve toxic chemical substances. Metallic nanoparticles have important applications in medical field, specially the gold ones. Through the nanoparticles biosynthesis using P. polymyxa and P. terrae, the process was optimized considering the synthesis time, the extract concentration, the wavelength and the particle size. The experiment was divided into phases where the best focus (1, 1: 2, 1: 4, 1: 8 and 1:16) was analyzed, observing through UV-Vis spectroscopy (without visible ultraviolate) at 540 nm the best absorption of light (how much) in relation to time and over 72 hours in a 12-hour interval. As the best indicators were 1: 2 and 1: 4, for both phyto-bacterial extracts and 12 to 24 hours as the best periods. The next phase was analyzed hourly for 24 hours, with the values 1: 2 and 1: 4, for (in relation to) light absorption at 540 nm and in the spectral range of 300 to 800 nm, demonstrating a statistic of gold nanoparticle for 1: 2 dilution, whose peak at around 540 nm, and as of 7 am are already described. Based on the results of the characterization by DLS (Dynamic Light Scattering - Dynamic Light Scattering) and absorbance, such as gold nanoparticles synthesized through crude extractions PP 1: 2 and PT 1: 2 were as obtaining better results, obtaining the average level of 262 nm and 372 nm, respectively, in monodispersed systems, with the following analyzes (occurrences) after 7 hours of biosynthesis reaction, obtaining greater results between 12 and 24 hours. The main absorption was detected at the red spectral range. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-11-12T17:00:39Z |
dc.date.available.fl_str_mv |
2020-11-12T17:00:39Z |
dc.date.issued.fl_str_mv |
2020-03-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARVALHO, Carina Miranda. Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae. 2020. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13430. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/13430 |
identifier_str_mv |
CARVALHO, Carina Miranda. Biossíntese de nanopartículas de ouro por Paenibacillus polymyxa e Paenibacillus terrae. 2020. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2020. Disponível em: https://repositorio.ufscar.br/handle/ufscar/13430. |
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https://repositorio.ufscar.br/handle/ufscar/13430 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Biotecnologia - PPGBiotec |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Universidade Federal de São Carlos (UFSCAR) |
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