Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/4075 |
Resumo: | This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a de-lignified substrate model; sugarcane bagasse (SB) steam exploded; and acid treated SB, the last two treated with 4% NaOH. All SB were chemically characterized. Hydrolysis experiments to study the influence of agitation and substrate concentration were performed in shaker, using Accellerase® 1500, Genencor, at pH 4.8, in 50mM sodium citrate buffer. To verify the substrate concentration effect, cellulose load (weightsubstrate/weighttotal) was 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP, the role of the external mass transport resistance was not significant when the agitation speed was in the range 150-300 rpm. It was possible to fit a pseudo-homogeneous Michaelis-Menten model for substrate concentrations up to 13% (w/w). Preliminary tests fitting the model proposed by Chrastil (CHRASTIL J. Enzymic product formation curves with the normal or diffusion limited reaction mechanism and in the presence of substrate receptors, Int. J. Biochem., v. 20, No. 7, p. 683, 1988) indicated that the role of diffusion through the external film was a more relevant feature for steam exploded SB than for FP, at higher concentrations of substrate. It was possible to fit a pseudo-homogeneous model for steam exploded SB, within a range of cellulose concentrations from 0.99% to 3.85% (w/w). Product inhibition had to be considered by the model. For higher loads of steam exploded SB, a modified Michaelis- Menten model, appropriate for heterogeneous systems with high diffusion resistance, was fitted. Finally, for the highly recalcitrant acid treated SB, Chrastil models were fitted. As expected, the complexity of this system regarding to the substrate and to the pool of enzymes acting in synergy, makes difficult the use of one single lumped parameter model for all hydrolysis operation conditions. |
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Carvalho, Mirella Lucas deGiordano, Raquel de Lima Camargohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4780181P0http://lattes.cnpq.br/3375214335297840d2265446-f3bc-4a9a-b095-7cbe8382e8782016-06-02T19:56:44Z2011-05-272016-06-02T19:56:44Z2011-03-28CARVALHO, Mirella Lucas de. Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar. 2011. 103 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011.https://repositorio.ufscar.br/handle/ufscar/4075This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a de-lignified substrate model; sugarcane bagasse (SB) steam exploded; and acid treated SB, the last two treated with 4% NaOH. All SB were chemically characterized. Hydrolysis experiments to study the influence of agitation and substrate concentration were performed in shaker, using Accellerase® 1500, Genencor, at pH 4.8, in 50mM sodium citrate buffer. To verify the substrate concentration effect, cellulose load (weightsubstrate/weighttotal) was 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP, the role of the external mass transport resistance was not significant when the agitation speed was in the range 150-300 rpm. It was possible to fit a pseudo-homogeneous Michaelis-Menten model for substrate concentrations up to 13% (w/w). Preliminary tests fitting the model proposed by Chrastil (CHRASTIL J. Enzymic product formation curves with the normal or diffusion limited reaction mechanism and in the presence of substrate receptors, Int. J. Biochem., v. 20, No. 7, p. 683, 1988) indicated that the role of diffusion through the external film was a more relevant feature for steam exploded SB than for FP, at higher concentrations of substrate. It was possible to fit a pseudo-homogeneous model for steam exploded SB, within a range of cellulose concentrations from 0.99% to 3.85% (w/w). Product inhibition had to be considered by the model. For higher loads of steam exploded SB, a modified Michaelis- Menten model, appropriate for heterogeneous systems with high diffusion resistance, was fitted. Finally, for the highly recalcitrant acid treated SB, Chrastil models were fitted. As expected, the complexity of this system regarding to the substrate and to the pool of enzymes acting in synergy, makes difficult the use of one single lumped parameter model for all hydrolysis operation conditions.Este trabalho apresenta um estudo cinético da hidrólise enzimática de três substratos celulósicos: papel de filtro (PF), usado como um modelo de substrato delignificado; bagaço de cana (BC) explodido a vapor e BC tratado com ácido, os dois últimos tratados com NaOH 4%. Todos os BC foram caracterizados. Experimentos de hidrólise para estudar a influência da agitação e da concentração do substrato foram realizados em shaker, usando Accellerase ® 1500, Genencor, em pH 4,8, em tampão citrato de sódio 50mM. Para verificar o efeito da concentração do substrato, a carga de celulose (m / m) foi de 0,5% -13% (para PF) e 0,99% - 9,09% (para o BC). Para o PF, o papel da resistência externa ao transporte de massa não foi significativo quando a velocidade de agitação foi na faixa de 150-300 rpm, em shaker. Foi possível ajustar um modelo pseudo-homogêneo de Michaelis-Menten para concentrações de substrato até 13% (m / m). Testes preliminares através do ajuste do modelo proposto por Chrastil (CHRASTIL J. Enzymic product formation curves with the normal or diffusion limited reaction mechanism and in the presence of substrate receptors, Int. J. Biochem., v. 20, No. 7, p. 683, 1988) indicaram que o papel da difusão através da película externa era uma característica mais relevante para o BC explodido a vapor que para o PF, em altas concentrações de substrato. Foi possível ajustar um modelo pseudo-homogêneo para o BC explodido a vapor, dentro de uma faixa de concentrações de celulose de 0,99% a 3,85% (m / m). Inibição pelo produto teve de ser considerada pelo modelo. Para cargas maiores de BC explodido a vapor, um modelo modificado de Michaelis-Menten, adequado para sistemas heterogêneos, com alta resistência à difusão, foi ajustado. Finalmente, para BC tratado com ácido (altamente recalcitrante), modelos de Chrastil foram ajustados. Como esperado, a complexidade deste sistema em relação ao substrato para o pool de enzimas agindo em sinergia, torna difícil o uso de um modelo único para todas as condições operacionais de hidrólise.Universidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Engenharia Química - PPGEQUFSCarBREngenharia químicaHidrólise de celuloseBagaço de canaEstudo cinéticoCellulose hydrolysisSugarcane bagasseKinetic studyENGENHARIAS::ENGENHARIA QUIMICAEstudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcarinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-187b60e6c-591e-4a38-94f3-e75e2beebea0info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL3648.pdfapplication/pdf4034707https://repositorio.ufscar.br/bitstream/ufscar/4075/1/3648.pdfd61c1d29df91b4f878b199e7de814791MD51THUMBNAIL3648.pdf.jpg3648.pdf.jpgIM Thumbnailimage/jpeg8406https://repositorio.ufscar.br/bitstream/ufscar/4075/2/3648.pdf.jpg0720dcf5bd4083927560385c8598cfaeMD52ufscar/40752023-09-18 18:30:59.533oai:repositorio.ufscar.br:ufscar/4075Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:59Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
title |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
spellingShingle |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar Carvalho, Mirella Lucas de Engenharia química Hidrólise de celulose Bagaço de cana Estudo cinético Cellulose hydrolysis Sugarcane bagasse Kinetic study ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
title_full |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
title_fullStr |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
title_full_unstemmed |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
title_sort |
Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar |
author |
Carvalho, Mirella Lucas de |
author_facet |
Carvalho, Mirella Lucas de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/3375214335297840 |
dc.contributor.author.fl_str_mv |
Carvalho, Mirella Lucas de |
dc.contributor.advisor1.fl_str_mv |
Giordano, Raquel de Lima Camargo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4780181P0 |
dc.contributor.authorID.fl_str_mv |
d2265446-f3bc-4a9a-b095-7cbe8382e878 |
contributor_str_mv |
Giordano, Raquel de Lima Camargo |
dc.subject.por.fl_str_mv |
Engenharia química Hidrólise de celulose Bagaço de cana Estudo cinético |
topic |
Engenharia química Hidrólise de celulose Bagaço de cana Estudo cinético Cellulose hydrolysis Sugarcane bagasse Kinetic study ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Cellulose hydrolysis Sugarcane bagasse Kinetic study |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA QUIMICA |
description |
This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a de-lignified substrate model; sugarcane bagasse (SB) steam exploded; and acid treated SB, the last two treated with 4% NaOH. All SB were chemically characterized. Hydrolysis experiments to study the influence of agitation and substrate concentration were performed in shaker, using Accellerase® 1500, Genencor, at pH 4.8, in 50mM sodium citrate buffer. To verify the substrate concentration effect, cellulose load (weightsubstrate/weighttotal) was 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP, the role of the external mass transport resistance was not significant when the agitation speed was in the range 150-300 rpm. It was possible to fit a pseudo-homogeneous Michaelis-Menten model for substrate concentrations up to 13% (w/w). Preliminary tests fitting the model proposed by Chrastil (CHRASTIL J. Enzymic product formation curves with the normal or diffusion limited reaction mechanism and in the presence of substrate receptors, Int. J. Biochem., v. 20, No. 7, p. 683, 1988) indicated that the role of diffusion through the external film was a more relevant feature for steam exploded SB than for FP, at higher concentrations of substrate. It was possible to fit a pseudo-homogeneous model for steam exploded SB, within a range of cellulose concentrations from 0.99% to 3.85% (w/w). Product inhibition had to be considered by the model. For higher loads of steam exploded SB, a modified Michaelis- Menten model, appropriate for heterogeneous systems with high diffusion resistance, was fitted. Finally, for the highly recalcitrant acid treated SB, Chrastil models were fitted. As expected, the complexity of this system regarding to the substrate and to the pool of enzymes acting in synergy, makes difficult the use of one single lumped parameter model for all hydrolysis operation conditions. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-05-27 2016-06-02T19:56:44Z |
dc.date.issued.fl_str_mv |
2011-03-28 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:56:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARVALHO, Mirella Lucas de. Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar. 2011. 103 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/4075 |
identifier_str_mv |
CARVALHO, Mirella Lucas de. Estudo cinético da hidrólise enzimática de celulose de bagaço de cana-de-açúcar. 2011. 103 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
url |
https://repositorio.ufscar.br/handle/ufscar/4075 |
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por |
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por |
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-1 -1 |
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87b60e6c-591e-4a38-94f3-e75e2beebea0 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de São Carlos |
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Programa de Pós-Graduação em Engenharia Química - PPGEQ |
dc.publisher.initials.fl_str_mv |
UFSCar |
dc.publisher.country.fl_str_mv |
BR |
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Universidade Federal de São Carlos |
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