Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/6231 |
Resumo: | First, a glassy carbon electrode modified with functionalized carbon nanotubes (CNTs) and glucose oxidase was proposed. Cyclic voltammetry of GOx immobilized onto the surface of CNTs showed a pair of well-defined redox peaks, which corresponds to the direct electron transfer of GOx, with a formal potential of -0.418 V in 0.1 mol L-1 phosphate buffer solution (pH 7.0). An apparent heterogeneous electron transfer rate constant of 1.69 s-1 was obtained. The determination of glucose was carried out by square wave voltammetry and the developed biosensor showed good reproducibility and stability. The second biosensor was a boron-doped diamond electrode modified with AuNPs electrodeposited and Tyrosinase (Tyr). The occurrence of direct electron transfer between the electrode surface and the Tyr active site was verified by cyclic voltammetry, yielding the following parameter values: formal redox potential of 0.115 V. The developed Tyr-AuNPs/BDD biosensor exhibits good sensitivity, stability, and reproducibility for the determination of phenol by SWV. The third proposed biosensor was developed based on acetylcholinesterase, gold nanoparticles and poly (allylamine hydrochloride) film on the surface of screen printed carbon electrodes (SPCE) for the determination of pesticides using a portable micromachined flow injection system. The AChE-AuNPs/SPCE biosensor presented a limit of detection of 5.0 x 10-8 mol L-1 for Diuron. In addition, the biosensor showed good sensitivity, stability and reproducibility for Diuron determination in water samples. Finally, a novel electrochemical immunosensor using electrodeposited gold nanoparticles modified-screen-printed carbon electrodes (AuNPs/SPCEs) was developed for the determination of prolactin.The variables involved in the preparation of the immunosensor and the electrochemical detection step were optimized, which presented a limit of detection of 10 ng mL-1. The analytical usefulness of the immnunosensor for the analysis of real samples was demonstrated by analyzing human serum spiked with PRL at two different concentration levels. |
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Janegitz, Bruno CamposFatibello Filho, OrlandoÚltima atualização do currículo em 14/03/2011http://lattes.cnpq.br/7746094938977989c1bc3574-4759-489b-8fde-5296d60427ad2016-06-02T20:34:35Z2012-08-292016-06-02T20:34:35Z2012-06-22JANEGITZ, Bruno Campos. Development of biosensors using carbon nanotubes and gold nanoparticles. 2012. 149 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2012.https://repositorio.ufscar.br/handle/ufscar/6231First, a glassy carbon electrode modified with functionalized carbon nanotubes (CNTs) and glucose oxidase was proposed. Cyclic voltammetry of GOx immobilized onto the surface of CNTs showed a pair of well-defined redox peaks, which corresponds to the direct electron transfer of GOx, with a formal potential of -0.418 V in 0.1 mol L-1 phosphate buffer solution (pH 7.0). An apparent heterogeneous electron transfer rate constant of 1.69 s-1 was obtained. The determination of glucose was carried out by square wave voltammetry and the developed biosensor showed good reproducibility and stability. The second biosensor was a boron-doped diamond electrode modified with AuNPs electrodeposited and Tyrosinase (Tyr). The occurrence of direct electron transfer between the electrode surface and the Tyr active site was verified by cyclic voltammetry, yielding the following parameter values: formal redox potential of 0.115 V. The developed Tyr-AuNPs/BDD biosensor exhibits good sensitivity, stability, and reproducibility for the determination of phenol by SWV. The third proposed biosensor was developed based on acetylcholinesterase, gold nanoparticles and poly (allylamine hydrochloride) film on the surface of screen printed carbon electrodes (SPCE) for the determination of pesticides using a portable micromachined flow injection system. The AChE-AuNPs/SPCE biosensor presented a limit of detection of 5.0 x 10-8 mol L-1 for Diuron. In addition, the biosensor showed good sensitivity, stability and reproducibility for Diuron determination in water samples. Finally, a novel electrochemical immunosensor using electrodeposited gold nanoparticles modified-screen-printed carbon electrodes (AuNPs/SPCEs) was developed for the determination of prolactin.The variables involved in the preparation of the immunosensor and the electrochemical detection step were optimized, which presented a limit of detection of 10 ng mL-1. The analytical usefulness of the immnunosensor for the analysis of real samples was demonstrated by analyzing human serum spiked with PRL at two different concentration levels.Primeiramente desenvolveu-se uma nova arquitetura de um biossensor para glicose utilizando-se a enzima glicose oxidase (GOx) imobilizada em nanotubos de carbono funcionalizados (CNTs) e dihexadecil hidrogenio fosfato (DHP) sobre a superficie de carbono vitreo (GCE). Resultados de voltametria ciclica apresentaram um par de picos redox bem definidos, correspondente a transferencia eletronica direta da GOx para o eletrodo, com o potencial formal de -0,418 V (vs. Ag/AgCl (KCl 3,0 mol L-1)). O biossensor foi aplicado na determinacao de glicose, o qual mostrou boas reprodutibilidade, estabilidade e seletividade. O segundo biossensor desenvolvido foi aquele utilizando-se a enzima tirosinase (Tyr) imobilizada em nanoparticulas de ouro sobre a superficie de um eletrodo de diamante dopado com boro. Resultados de voltametria ciclica apresentaram um par de picos redox bem definidos, correspondente a transferencia eletronica direta da Tyr. As determinacoes de fenol foram realizadas por SWV e o biossensor proposto apresentou um limite de deteccao de 0,07 μmol L-1. Em sequencia foi proposto um biossensor para a determinacao de pesticidas utilizando-se a enzima acetilcolinesterase imobilizada sobre filme de nanoparticulas de ouro e hidrocloreto de polialilamina sobre a superficie de eletrodos de carbono impresso (SPCE). O eletrodo AChE-AuNPs/SPCE apresentou um limite de deteccao de 5,0 x 10-8 mol L-1 para Diuron, alem de boa estabilidade e reprodutibilidade para a determinacao do pesticida por amperometria. Por fim foi proposto tambem um imunossensor usando nanoparticulas de ouro eletrodepositadas sobre eletrodos de carbono impresso (AuNPs/SPCEs) para a determinacao de prolactina. Os anticorpos antiprolactina biotinilada foram imobilizados em AuNPs/SPCEs modificados com estreptavidina em um imunoensaio do tipo sanduiche envolvendo o analito e um anticorpo antiprolactina marcado com fosfatase alcalina (AP). O imunossensor proposto apresentou um limite de deteccao de 10 ng mL-1 e foi aplicado com sucesso em amostras de soro humano enriquecido com prolactina comprovando a eficacia do procedimento proposto.Universidade Federal de Minas Geraisapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarBRQuímica analíticaBiosensores (Biossensores)Nanotubos de carbonoNanopartículas de ouroMateriais nanoestruturadosCIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICADesenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouroDevelopment of biosensors using carbon nanotubes and gold nanoparticlesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-1-17a1dec22-4b2d-4dba-8971-ab07f55c7d54info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL4417.pdfapplication/pdf4764292https://repositorio.ufscar.br/bitstream/ufscar/6231/1/4417.pdff843bb063e4e62585bcaf3a316f53db2MD51TEXT4417.pdf.txt4417.pdf.txtExtracted texttext/plain0https://repositorio.ufscar.br/bitstream/ufscar/6231/4/4417.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD54THUMBNAIL4417.pdf.jpg4417.pdf.jpgIM Thumbnailimage/jpeg9714https://repositorio.ufscar.br/bitstream/ufscar/6231/5/4417.pdf.jpg440cb1dc8b5aee9b18da14694eee9912MD55ufscar/62312023-09-18 18:30:32.558oai:repositorio.ufscar.br:ufscar/6231Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:32Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
dc.title.alternative.eng.fl_str_mv |
Development of biosensors using carbon nanotubes and gold nanoparticles |
title |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
spellingShingle |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro Janegitz, Bruno Campos Química analítica Biosensores (Biossensores) Nanotubos de carbono Nanopartículas de ouro Materiais nanoestruturados CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA |
title_short |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
title_full |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
title_fullStr |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
title_full_unstemmed |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
title_sort |
Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas de ouro |
author |
Janegitz, Bruno Campos |
author_facet |
Janegitz, Bruno Campos |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/7746094938977989 |
dc.contributor.author.fl_str_mv |
Janegitz, Bruno Campos |
dc.contributor.advisor1.fl_str_mv |
Fatibello Filho, Orlando |
dc.contributor.advisor1Lattes.fl_str_mv |
Última atualização do currículo em 14/03/2011 |
dc.contributor.authorID.fl_str_mv |
c1bc3574-4759-489b-8fde-5296d60427ad |
contributor_str_mv |
Fatibello Filho, Orlando |
dc.subject.por.fl_str_mv |
Química analítica Biosensores (Biossensores) Nanotubos de carbono Nanopartículas de ouro Materiais nanoestruturados |
topic |
Química analítica Biosensores (Biossensores) Nanotubos de carbono Nanopartículas de ouro Materiais nanoestruturados CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA |
description |
First, a glassy carbon electrode modified with functionalized carbon nanotubes (CNTs) and glucose oxidase was proposed. Cyclic voltammetry of GOx immobilized onto the surface of CNTs showed a pair of well-defined redox peaks, which corresponds to the direct electron transfer of GOx, with a formal potential of -0.418 V in 0.1 mol L-1 phosphate buffer solution (pH 7.0). An apparent heterogeneous electron transfer rate constant of 1.69 s-1 was obtained. The determination of glucose was carried out by square wave voltammetry and the developed biosensor showed good reproducibility and stability. The second biosensor was a boron-doped diamond electrode modified with AuNPs electrodeposited and Tyrosinase (Tyr). The occurrence of direct electron transfer between the electrode surface and the Tyr active site was verified by cyclic voltammetry, yielding the following parameter values: formal redox potential of 0.115 V. The developed Tyr-AuNPs/BDD biosensor exhibits good sensitivity, stability, and reproducibility for the determination of phenol by SWV. The third proposed biosensor was developed based on acetylcholinesterase, gold nanoparticles and poly (allylamine hydrochloride) film on the surface of screen printed carbon electrodes (SPCE) for the determination of pesticides using a portable micromachined flow injection system. The AChE-AuNPs/SPCE biosensor presented a limit of detection of 5.0 x 10-8 mol L-1 for Diuron. In addition, the biosensor showed good sensitivity, stability and reproducibility for Diuron determination in water samples. Finally, a novel electrochemical immunosensor using electrodeposited gold nanoparticles modified-screen-printed carbon electrodes (AuNPs/SPCEs) was developed for the determination of prolactin.The variables involved in the preparation of the immunosensor and the electrochemical detection step were optimized, which presented a limit of detection of 10 ng mL-1. The analytical usefulness of the immnunosensor for the analysis of real samples was demonstrated by analyzing human serum spiked with PRL at two different concentration levels. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-08-29 2016-06-02T20:34:35Z |
dc.date.issued.fl_str_mv |
2012-06-22 |
dc.date.accessioned.fl_str_mv |
2016-06-02T20:34:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
JANEGITZ, Bruno Campos. Development of biosensors using carbon nanotubes and gold nanoparticles. 2012. 149 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/6231 |
identifier_str_mv |
JANEGITZ, Bruno Campos. Development of biosensors using carbon nanotubes and gold nanoparticles. 2012. 149 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2012. |
url |
https://repositorio.ufscar.br/handle/ufscar/6231 |
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por |
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openAccess |
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Universidade Federal de São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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BR |
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