Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/6197 |
Resumo: | The present work describes the study of variation in the chemical profile of citrus after inoculation of Xylella fastidiosa, in charge of the Citrus Variegated Chlorosis is one of the most serious diseases that cause damage in Brazilian citrus. The bacterium X. fastidiosa was inoculated in Citrus sinensis grafted on C. limonia and was evaluated using chemometric tools and development of analytical methods such as HPLC-DAD, LC-MS/MS, LC-UV-SPE-NMR and HRMAS quantitative variation of some secondary metabolites such as coumarins and flavonoids identified both in grafts and in the matrix C. sinensis and C. limonia. The chemometric methods PCA and HCA applied to the data obtained in studies by HPLC-DAD and HR-MAS allowed to discriminate plants contaminated with the bacteria of the species healthy, as well as the respective individuals. By analyzing the change in the chemical profile of citrus studied by HPLC-UV-SPE-NMR it was possible to detect the presence of a new class of secondary metabolites derived from p-hidroxy-cinnamic acid containing oxidized dihydropyran and dihydrofuran rings. Similarly, when analyzing the compounds of medium polarity by LC-MS/MS technique was identified, until now, in the roots and leaves several coumarins not cited in the literature for any of the species that constitute the graft, as dihydrosuberenol, 2 ', 3'-dihydrosuberosin, citrubutin the limonoid diosfenol limonin and flavonoid 3-hydroxy-3', 4 ',5,7,8- pentamethoxyflavone. Finally, was observed that coumarin bergapten is present in reasonable quantities only in the leaves of Rangpur lime, which is tolerant to X. fastidiosa, and when the graft presents with symptoms of citrus variegated chlorosis is a small production of this in their leaves, indicating that this is a phytoanticipin. |
id |
SCAR_5d3614f36b38f5caa7af380a718413f2 |
---|---|
oai_identifier_str |
oai:repositorio.ufscar.br:ufscar/6197 |
network_acronym_str |
SCAR |
network_name_str |
Repositório Institucional da UFSCAR |
repository_id_str |
4322 |
spelling |
Soares, Márcio SantosSilva, Maria Fátima das Graças Fernandes dahttp://lattes.cnpq.br/0457632122660653http://lattes.cnpq.br/72776720463135440c3f2c0d-2ae9-413b-a3b9-0774f9c6483c2016-06-02T20:34:26Z2011-10-102016-06-02T20:34:26Z2011-06-22SOARES, Márcio Santos. Study of the variation in the chemical profile induced in citrus sinensis grafted onto c. Limonia by inoculation with xylella fastidiosa. 2011. 357 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011.https://repositorio.ufscar.br/handle/ufscar/6197The present work describes the study of variation in the chemical profile of citrus after inoculation of Xylella fastidiosa, in charge of the Citrus Variegated Chlorosis is one of the most serious diseases that cause damage in Brazilian citrus. The bacterium X. fastidiosa was inoculated in Citrus sinensis grafted on C. limonia and was evaluated using chemometric tools and development of analytical methods such as HPLC-DAD, LC-MS/MS, LC-UV-SPE-NMR and HRMAS quantitative variation of some secondary metabolites such as coumarins and flavonoids identified both in grafts and in the matrix C. sinensis and C. limonia. The chemometric methods PCA and HCA applied to the data obtained in studies by HPLC-DAD and HR-MAS allowed to discriminate plants contaminated with the bacteria of the species healthy, as well as the respective individuals. By analyzing the change in the chemical profile of citrus studied by HPLC-UV-SPE-NMR it was possible to detect the presence of a new class of secondary metabolites derived from p-hidroxy-cinnamic acid containing oxidized dihydropyran and dihydrofuran rings. Similarly, when analyzing the compounds of medium polarity by LC-MS/MS technique was identified, until now, in the roots and leaves several coumarins not cited in the literature for any of the species that constitute the graft, as dihydrosuberenol, 2 ', 3'-dihydrosuberosin, citrubutin the limonoid diosfenol limonin and flavonoid 3-hydroxy-3', 4 ',5,7,8- pentamethoxyflavone. Finally, was observed that coumarin bergapten is present in reasonable quantities only in the leaves of Rangpur lime, which is tolerant to X. fastidiosa, and when the graft presents with symptoms of citrus variegated chlorosis is a small production of this in their leaves, indicating that this is a phytoanticipin.O presente trabalho descreve o estudo da variação do perfil químico de citros após a inoculação da bactéria Xylella fastidiosa, responsável pela Clorose Variegada do Citros (CVC) que é uma das mais sérias doenças causadoras de prejuízos na citricultura brasileira. Para isso, inoculou-se a X. fastidiosa em enxertos de C. sinensis sobre C. limonia e avaliou-se através de ferramentas quimiométricas e de desenvolvimento de métodos analíticos como HPLC-DAD, LC-MS/MS, LC-UV-SPE-NMR e HRMAS a variação quantitativa de alguns metabólitos secundários, como cumarinas e flavonóides identificados tanto nos enxertos quanto nas matrizes C. sinensis e C. limonia. As ferramentas quimiométricas PCA e HCA aplicadas aos dados obtidos nos estudos de HPLC-DAD e HR-MAS permitiram discriminar as plantas contaminadas com a bactéria das espécies sadias, assim como os respectivos indivíduos. Ao analisar a variação do perfil químico dos citros estudados através da HPLC-SPE-UVNMR foi possível detectar a presença de uma nova classe de metabólitos secundários derivados do ácido p-metoxicinâmico contendo anéis diidropirano e diidrofurano oxidados. Do mesmo modo, ao analisar os compostos de média polaridade, através da técnica de LC-MS/MS foi possível identificar, até o presente momento, nas raízes e folhas algumas cumarinas não citadas na literatura para nenhuma das duas espécies que constituem o enxerto, como diidrosuberenol, 2 ,3 -diidrosuberosina, citrubutina, o limonóide limonina diosfenol e o flavonóide 3-hidroxi- 3 ,4 ,5,7,8-pentametoxiflavona. Finalmente, observou-se que a cumarina bergapteno está presente em quantidade razoável apenas nas folhas do limão cravo, o qual é tolerante à X. fastidiosa, e quando o enxerto apresenta os sintomas da CVC há uma pequena produção desta em suas folhas, indicando ser esta uma fitoantecipinaUniversidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarBRProdutos naturaisFitoalexinaPerfil químicoCumarinasCromatografia líquida de alta eficiênciaCIENCIAS EXATAS E DA TERRA::QUIMICAEstudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosaStudy of the variation in the chemical profile induced in citrus sinensis grafted onto c. Limonia by inoculation with xylella fastidiosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-1-124b0ac94-885a-4e7b-b93a-7cad21b9592einfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL3753.pdfapplication/pdf9161600https://repositorio.ufscar.br/bitstream/ufscar/6197/1/3753.pdf26887a20430e04deb54cb6b8e7360500MD51THUMBNAIL3753.pdf.jpg3753.pdf.jpgIM Thumbnailimage/jpeg10648https://repositorio.ufscar.br/bitstream/ufscar/6197/2/3753.pdf.jpgf8172b5940ce1b548c562541fa3899fcMD52ufscar/61972023-09-18 18:31:10.076oai:repositorio.ufscar.br:ufscar/6197Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:10Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
dc.title.alternative.eng.fl_str_mv |
Study of the variation in the chemical profile induced in citrus sinensis grafted onto c. Limonia by inoculation with xylella fastidiosa |
title |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
spellingShingle |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa Soares, Márcio Santos Produtos naturais Fitoalexina Perfil químico Cumarinas Cromatografia líquida de alta eficiência CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
title_full |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
title_fullStr |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
title_full_unstemmed |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
title_sort |
Estudo da variação do perfil químico de Citrus sinensis sobre Citrus limonia após a inoculação da bactéria Xylella fastidiosa |
author |
Soares, Márcio Santos |
author_facet |
Soares, Márcio Santos |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/7277672046313544 |
dc.contributor.author.fl_str_mv |
Soares, Márcio Santos |
dc.contributor.advisor1.fl_str_mv |
Silva, Maria Fátima das Graças Fernandes da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0457632122660653 |
dc.contributor.authorID.fl_str_mv |
0c3f2c0d-2ae9-413b-a3b9-0774f9c6483c |
contributor_str_mv |
Silva, Maria Fátima das Graças Fernandes da |
dc.subject.por.fl_str_mv |
Produtos naturais Fitoalexina Perfil químico Cumarinas Cromatografia líquida de alta eficiência |
topic |
Produtos naturais Fitoalexina Perfil químico Cumarinas Cromatografia líquida de alta eficiência CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The present work describes the study of variation in the chemical profile of citrus after inoculation of Xylella fastidiosa, in charge of the Citrus Variegated Chlorosis is one of the most serious diseases that cause damage in Brazilian citrus. The bacterium X. fastidiosa was inoculated in Citrus sinensis grafted on C. limonia and was evaluated using chemometric tools and development of analytical methods such as HPLC-DAD, LC-MS/MS, LC-UV-SPE-NMR and HRMAS quantitative variation of some secondary metabolites such as coumarins and flavonoids identified both in grafts and in the matrix C. sinensis and C. limonia. The chemometric methods PCA and HCA applied to the data obtained in studies by HPLC-DAD and HR-MAS allowed to discriminate plants contaminated with the bacteria of the species healthy, as well as the respective individuals. By analyzing the change in the chemical profile of citrus studied by HPLC-UV-SPE-NMR it was possible to detect the presence of a new class of secondary metabolites derived from p-hidroxy-cinnamic acid containing oxidized dihydropyran and dihydrofuran rings. Similarly, when analyzing the compounds of medium polarity by LC-MS/MS technique was identified, until now, in the roots and leaves several coumarins not cited in the literature for any of the species that constitute the graft, as dihydrosuberenol, 2 ', 3'-dihydrosuberosin, citrubutin the limonoid diosfenol limonin and flavonoid 3-hydroxy-3', 4 ',5,7,8- pentamethoxyflavone. Finally, was observed that coumarin bergapten is present in reasonable quantities only in the leaves of Rangpur lime, which is tolerant to X. fastidiosa, and when the graft presents with symptoms of citrus variegated chlorosis is a small production of this in their leaves, indicating that this is a phytoanticipin. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-10-10 2016-06-02T20:34:26Z |
dc.date.issued.fl_str_mv |
2011-06-22 |
dc.date.accessioned.fl_str_mv |
2016-06-02T20:34:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOARES, Márcio Santos. Study of the variation in the chemical profile induced in citrus sinensis grafted onto c. Limonia by inoculation with xylella fastidiosa. 2011. 357 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/6197 |
identifier_str_mv |
SOARES, Márcio Santos. Study of the variation in the chemical profile induced in citrus sinensis grafted onto c. Limonia by inoculation with xylella fastidiosa. 2011. 357 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
url |
https://repositorio.ufscar.br/handle/ufscar/6197 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.confidence.fl_str_mv |
-1 -1 |
dc.relation.authority.fl_str_mv |
24b0ac94-885a-4e7b-b93a-7cad21b9592e |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química - PPGQ |
dc.publisher.initials.fl_str_mv |
UFSCar |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFSCAR instname:Universidade Federal de São Carlos (UFSCAR) instacron:UFSCAR |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
UFSCAR |
institution |
UFSCAR |
reponame_str |
Repositório Institucional da UFSCAR |
collection |
Repositório Institucional da UFSCAR |
bitstream.url.fl_str_mv |
https://repositorio.ufscar.br/bitstream/ufscar/6197/1/3753.pdf https://repositorio.ufscar.br/bitstream/ufscar/6197/2/3753.pdf.jpg |
bitstream.checksum.fl_str_mv |
26887a20430e04deb54cb6b8e7360500 f8172b5940ce1b548c562541fa3899fc |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
|
_version_ |
1802136292290461696 |