Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/2099 |
Resumo: | The diflubenzuron (DFB), sold under the name Dimilin® (CHEMTURA), has been extensively used in aquaculture and agricultural applications may occur contamination of the aquatic environment. Prochilodus lineatus was exposed to 0.0, 0.25, 0.5, 1.0 and 2.0 mg L-1 DFB for 14 days and then were determined frequency erythrocytic nuclear abnormalities (ANE), and micronuclei (MN) in the blood, lipid peroxidation (LPO) in the gills and liver, histopathology of gills and liver, total osmolarity and concentration of plasma ions, the activity of the enzymes Na+/k+-ATPase (NKA) and H+-ATPase in the gills and the activity of carbonic anhydrase (AC) in the gills. The most frequent ENA were the kidney-shaped nucleus and micronucleus and increased significantly in the groups exposed to 0.5, 1.0 and 2.0 mg L-1 DFB. The LPO concentration in the gills reduced in animals exposed to 2.0 mg L-1 DFB and in the liver there was an increase in the animals exposed to 0.5 and 2.0 mg L-1. The degree of tissue change (IAH), in the gills was higher in the groups exposed to 1.0 and 2.0 mg L-1 DFB. In the liver, the average change (VMA) and IAH increased in the groups exposed to 1.0 and 2.0 mg L-1 DFB. The total plasma osmolarity increased significantly in the group exposed to 0.5 mg L-1 Dimilin®, for the Cl- ion, there was a significant decrease of its concentration in the group exposed to 2.0 mg L-1. The activity of H+-ATPase showed an increase in group 1.0 mg L-1. The AC decreased significantly in the group exposed to 2.0 mg L-1. The Dimilin® has potential toxic to P. lineatus, causing genotoxic and mutangênica changes as well as changes morphofunctional in gills and liver, and can be monitored through the experimental techniques used in this work. |
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Benze, Tayrine PaschoalettiFernandes, Marisa Narcisohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6http://lattes.cnpq.br/93668821136498970eb6f54c-7a7d-4c29-949e-9a9788cac70d2016-06-02T19:32:08Z2014-02-172016-06-02T19:32:08Z2013-06-28BENZE, Tayrine Paschoaletti. Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2013. 74 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2013.https://repositorio.ufscar.br/handle/ufscar/2099The diflubenzuron (DFB), sold under the name Dimilin® (CHEMTURA), has been extensively used in aquaculture and agricultural applications may occur contamination of the aquatic environment. Prochilodus lineatus was exposed to 0.0, 0.25, 0.5, 1.0 and 2.0 mg L-1 DFB for 14 days and then were determined frequency erythrocytic nuclear abnormalities (ANE), and micronuclei (MN) in the blood, lipid peroxidation (LPO) in the gills and liver, histopathology of gills and liver, total osmolarity and concentration of plasma ions, the activity of the enzymes Na+/k+-ATPase (NKA) and H+-ATPase in the gills and the activity of carbonic anhydrase (AC) in the gills. The most frequent ENA were the kidney-shaped nucleus and micronucleus and increased significantly in the groups exposed to 0.5, 1.0 and 2.0 mg L-1 DFB. The LPO concentration in the gills reduced in animals exposed to 2.0 mg L-1 DFB and in the liver there was an increase in the animals exposed to 0.5 and 2.0 mg L-1. The degree of tissue change (IAH), in the gills was higher in the groups exposed to 1.0 and 2.0 mg L-1 DFB. In the liver, the average change (VMA) and IAH increased in the groups exposed to 1.0 and 2.0 mg L-1 DFB. The total plasma osmolarity increased significantly in the group exposed to 0.5 mg L-1 Dimilin®, for the Cl- ion, there was a significant decrease of its concentration in the group exposed to 2.0 mg L-1. The activity of H+-ATPase showed an increase in group 1.0 mg L-1. The AC decreased significantly in the group exposed to 2.0 mg L-1. The Dimilin® has potential toxic to P. lineatus, causing genotoxic and mutangênica changes as well as changes morphofunctional in gills and liver, and can be monitored through the experimental techniques used in this work.O diflubenzuron (DFB), comercializado com o nome de Dimilin® (CHEMTURA), tem sido intensivamente utilizado em aquicultura e aplicações agrícolas podendo ocorrer contaminação do ambiente aquático. Prochilodus lineatus foi exposto a 0,0, 0,25, 0,5, 1,0 e 2,0 mg L-1 de DFB durante 14 dias e, em seguida, foram determinadas a frequência de anormalidades nucleares eritrocíticas (ANE) e micronúcleos (MN) no sangue, a peroxidação lipídica (LPO) nas brânquias e fígado, histopatologias das brânquias e fígado, osmolaridade total e concentração dos íons plasmáticos, a atividade das enzimas Na+/K+-ATPase (NKA) e H+-ATPase nas brânquias e a atividade da anidrase carbônica (AC) nas brânquias. As ANE mais frequentes foram o núcleo em formato de rim e micronúcleo e aumentaram significativamente nos grupos expostas a 0,5, 1,0 e 2,0 mg L-1 de DFB. A concentração de LPO nas brânquias diminuiu nos animais expostos a 2,0 mg L-1 de DFB e no fígado ocorreu um aumento nos animais expostos a 0,5 e 2,0 mg L- 1. O índice de alteração histopatológico nas brânquias (IAH) foi maior nos grupos expostos a 1,0 e 2,0 mg L-1 de DFB. No fígado, o valor médio de alteração (VMA) e o IAH aumentaram nos grupos expostos a 1,0 e 2,0 mg L-1 de DFB. A osmolaridade total do plasma aumentou de forma significativa no grupo exposto a 0,5 mg L-1 de Dimilin®, em relação ao íon Cl-, houve uma diminuição significativa de sua concentração no grupo exposto a 2,0 mg L-1. A atividade da H+ - ATPase demonstrou um aumento no grupo 1,0 mg L-1. A AC diminuiu significativamente no grupo exposto a 2,0 mg L-1. O Dimilin® apresenta potencial tóxico para P. lineatus, causando alterações genotóxicas e mutagênicas, assim como alterações morfofuncionais nas brânquias e fígado, e pode ser monitorado através das técnicas experimentais utilizadas no presente trabalho.Universidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Ecologia e Recursos Naturais - PPGERNUFSCarBREcologia aquáticaEcotoxicologiaProchilodus lineatusDiflubenzuronAlterações histopatológicasMonitoramento ambientalCIENCIAS BIOLOGICAS::ECOLOGIAEfeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-15470807a-1e63-4591-8511-0cb223b337f2info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL5673.pdfapplication/pdf7713531https://repositorio.ufscar.br/bitstream/ufscar/2099/1/5673.pdfdfb51ed9dae115ec975880790cad5d10MD51THUMBNAIL5673.pdf.jpg5673.pdf.jpgIM Thumbnailimage/jpeg6458https://repositorio.ufscar.br/bitstream/ufscar/2099/2/5673.pdf.jpg1014178bc5c4541d82a2c8956d5794abMD52ufscar/20992023-09-18 18:30:48.257oai:repositorio.ufscar.br:ufscar/2099Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:48Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
spellingShingle |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) Benze, Tayrine Paschoaletti Ecologia aquática Ecotoxicologia Prochilodus lineatus Diflubenzuron Alterações histopatológicas Monitoramento ambiental CIENCIAS BIOLOGICAS::ECOLOGIA |
title_short |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_full |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_fullStr |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_full_unstemmed |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
title_sort |
Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae) |
author |
Benze, Tayrine Paschoaletti |
author_facet |
Benze, Tayrine Paschoaletti |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/9366882113649897 |
dc.contributor.author.fl_str_mv |
Benze, Tayrine Paschoaletti |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Marisa Narciso |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6 |
dc.contributor.authorID.fl_str_mv |
0eb6f54c-7a7d-4c29-949e-9a9788cac70d |
contributor_str_mv |
Fernandes, Marisa Narciso |
dc.subject.por.fl_str_mv |
Ecologia aquática Ecotoxicologia Prochilodus lineatus Diflubenzuron Alterações histopatológicas Monitoramento ambiental |
topic |
Ecologia aquática Ecotoxicologia Prochilodus lineatus Diflubenzuron Alterações histopatológicas Monitoramento ambiental CIENCIAS BIOLOGICAS::ECOLOGIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ECOLOGIA |
description |
The diflubenzuron (DFB), sold under the name Dimilin® (CHEMTURA), has been extensively used in aquaculture and agricultural applications may occur contamination of the aquatic environment. Prochilodus lineatus was exposed to 0.0, 0.25, 0.5, 1.0 and 2.0 mg L-1 DFB for 14 days and then were determined frequency erythrocytic nuclear abnormalities (ANE), and micronuclei (MN) in the blood, lipid peroxidation (LPO) in the gills and liver, histopathology of gills and liver, total osmolarity and concentration of plasma ions, the activity of the enzymes Na+/k+-ATPase (NKA) and H+-ATPase in the gills and the activity of carbonic anhydrase (AC) in the gills. The most frequent ENA were the kidney-shaped nucleus and micronucleus and increased significantly in the groups exposed to 0.5, 1.0 and 2.0 mg L-1 DFB. The LPO concentration in the gills reduced in animals exposed to 2.0 mg L-1 DFB and in the liver there was an increase in the animals exposed to 0.5 and 2.0 mg L-1. The degree of tissue change (IAH), in the gills was higher in the groups exposed to 1.0 and 2.0 mg L-1 DFB. In the liver, the average change (VMA) and IAH increased in the groups exposed to 1.0 and 2.0 mg L-1 DFB. The total plasma osmolarity increased significantly in the group exposed to 0.5 mg L-1 Dimilin®, for the Cl- ion, there was a significant decrease of its concentration in the group exposed to 2.0 mg L-1. The activity of H+-ATPase showed an increase in group 1.0 mg L-1. The AC decreased significantly in the group exposed to 2.0 mg L-1. The Dimilin® has potential toxic to P. lineatus, causing genotoxic and mutangênica changes as well as changes morphofunctional in gills and liver, and can be monitored through the experimental techniques used in this work. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-06-28 |
dc.date.available.fl_str_mv |
2014-02-17 2016-06-02T19:32:08Z |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:32:08Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BENZE, Tayrine Paschoaletti. Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2013. 74 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/2099 |
identifier_str_mv |
BENZE, Tayrine Paschoaletti. Efeitos da exposição ao inseticida diflubenzuron em curimbatá, Prochilodus lineatus (Teleósteo, Prochilodontidae). 2013. 74 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2013. |
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https://repositorio.ufscar.br/handle/ufscar/2099 |
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por |
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Universidade Federal de São Carlos |
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BR |
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