Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/6490 |
Resumo: | Alternanthera brasiliana is widely used by Brazilian people as a folk medicine for the treatment of numerous diseases. However, little is known about its chemical composition and the microorganisms that live in association with it. This work proposed the use of dereplication strategies to establish a correlation between chemical compounds present in the plant and the antibiotic activity associated with it. These strategies were also used for study endophytic micro-organisms associated with this plant, since the metabolites present in plant tissues are often result of interaction between plants and different factors present in their habitat, such as micro-organisms. 50 strains of endophytic micro-organisms associated to Alternanthera brasiliana were isolated. High-Throughput Screening strategies, which envolved analytical techniques as LC-PDA-MS, LC-PDA-MS/MS, LC-SPE-NMR and antibiosis assays allowed identification of antibiotic compounds present in the A. brasiliana stems. These compounds are member of oxylipins group, among which we highlight the 13-Z,E-KODE, 13-E,E-KODE, 9-E,Z-KODE and 9-E,EKODE. Furthermore, were identified some depsipeptides compounds produced by a strain of Fusarium fungus, isolated as endophyte of A. brasiliana. Beauvericin, which is one of these compounds , is known for its antibiotic and insecticide activities. This compound was also found in the plant extracts, suggesting that endophyte microorganism could play a role in producing antibiotic compounds inside plant tissues. |
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Trapp, Marília AlmeidaRodrigues Filho, Edsonhttp://lattes.cnpq.br/3667941735597178http://lattes.cnpq.br/40768943762187777ded997f-1a92-4b29-8952-d6e773aa2fc82016-06-02T20:36:31Z2011-05-122016-06-02T20:36:31Z2011-02-24TRAPP, Marília Almeida. Studies towards the dereplication of antibiotic constituents in Alternanthera brasiliana and associated microorganisms. 2011. 193 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011.https://repositorio.ufscar.br/handle/ufscar/6490Alternanthera brasiliana is widely used by Brazilian people as a folk medicine for the treatment of numerous diseases. However, little is known about its chemical composition and the microorganisms that live in association with it. This work proposed the use of dereplication strategies to establish a correlation between chemical compounds present in the plant and the antibiotic activity associated with it. These strategies were also used for study endophytic micro-organisms associated with this plant, since the metabolites present in plant tissues are often result of interaction between plants and different factors present in their habitat, such as micro-organisms. 50 strains of endophytic micro-organisms associated to Alternanthera brasiliana were isolated. High-Throughput Screening strategies, which envolved analytical techniques as LC-PDA-MS, LC-PDA-MS/MS, LC-SPE-NMR and antibiosis assays allowed identification of antibiotic compounds present in the A. brasiliana stems. These compounds are member of oxylipins group, among which we highlight the 13-Z,E-KODE, 13-E,E-KODE, 9-E,Z-KODE and 9-E,EKODE. Furthermore, were identified some depsipeptides compounds produced by a strain of Fusarium fungus, isolated as endophyte of A. brasiliana. Beauvericin, which is one of these compounds , is known for its antibiotic and insecticide activities. This compound was also found in the plant extracts, suggesting that endophyte microorganism could play a role in producing antibiotic compounds inside plant tissues.A planta Alternanthera brasiliana é popularmente conhecida por suas diversas atividades biológicas. Assim, o presente trabalho pretende contribuir com o uso de estratégias de desreplicação a fim de estabelecer uma correlação entre os compostos químicos presentes nesta planta e a atividade antibiótica associada a ela. Além disso, essas estratégias de desreplicação foram utilizadas para o estudo dos micro-organismos endofiticos associados a esta planta, uma vez que os metabólitos secundários presentes nos tecidos das plantas muitas vezes são resultado da interação desta planta com diversos fatores presentes em seu habitat, e dentre eles a interação com microorganismos merece destaque. Foram isoladas 50 cepas de micro-organismos endofiticos associados à planta Alternanthera brasiliana. O uso de abordagens de High Throughput Screening aliadas a técnicas analíticas como LC-PDA-MS, LC-PDA-MS/MS, LC-SPE-NMR e ensaios de antibiose permitiram a identificação dos compostos antibióticos presentes no caule de A. brasiliana. Esses compostos pertencem a classe das oxilipinas, dentre os quais se destacam o 13-Z,E-KODE, 13-E,E-KODE, 9-E,Z-KODE e 9-E,E-KODE. Tambem foram identificados compostos da classe dos depsipeptídeos nos extratos de uma cepa do gênero Fusarium, isolada como endofítico de A. brasiliana. Dentre os depsipeptídeos encontrados, a beauvericina estava presente em grande quantidade no extrato deste micro-organismo. Este composto, conhecido por suas atividades antibiótica e inseticida, foi encontrado também no extrato da planta, sugerindo que o micro-organismo endofítico poderia estar produzindo compostos desta classe no interior dos tecidos da planta, onde ele está alojado.Universidade Federal de Minas Geraisapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarBRProdutos naturaisMicroorganismos endofíticosEspectrometria de massasPlantas medicinaisCIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICAEstratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associadosStudies towards the dereplication of antibiotic constituents in Alternanthera brasiliana and associated microorganismsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-138736a86-d071-4ee1-8969-144f8d20b7ccinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL3601.pdfapplication/pdf23843273https://repositorio.ufscar.br/bitstream/ufscar/6490/1/3601.pdf9749b85bea7922b896f2c98d31db0fc3MD51THUMBNAIL3601.pdf.jpg3601.pdf.jpgIM Thumbnailimage/jpeg13551https://repositorio.ufscar.br/bitstream/ufscar/6490/2/3601.pdf.jpg4de0384eba148f565b0d5a3596154a0bMD52ufscar/64902023-09-18 18:31:11.766oai:repositorio.ufscar.br:ufscar/6490Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:11Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
dc.title.alternative.eng.fl_str_mv |
Studies towards the dereplication of antibiotic constituents in Alternanthera brasiliana and associated microorganisms |
title |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
spellingShingle |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados Trapp, Marília Almeida Produtos naturais Microorganismos endofíticos Espectrometria de massas Plantas medicinais CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA |
title_short |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
title_full |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
title_fullStr |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
title_full_unstemmed |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
title_sort |
Estratégias de desreplicação aplicadas ao estudo da planta Alternanthera brasiliana e dos fungos endofíticos associados |
author |
Trapp, Marília Almeida |
author_facet |
Trapp, Marília Almeida |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/4076894376218777 |
dc.contributor.author.fl_str_mv |
Trapp, Marília Almeida |
dc.contributor.advisor1.fl_str_mv |
Rodrigues Filho, Edson |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3667941735597178 |
dc.contributor.authorID.fl_str_mv |
7ded997f-1a92-4b29-8952-d6e773aa2fc8 |
contributor_str_mv |
Rodrigues Filho, Edson |
dc.subject.por.fl_str_mv |
Produtos naturais Microorganismos endofíticos Espectrometria de massas Plantas medicinais |
topic |
Produtos naturais Microorganismos endofíticos Espectrometria de massas Plantas medicinais CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA |
description |
Alternanthera brasiliana is widely used by Brazilian people as a folk medicine for the treatment of numerous diseases. However, little is known about its chemical composition and the microorganisms that live in association with it. This work proposed the use of dereplication strategies to establish a correlation between chemical compounds present in the plant and the antibiotic activity associated with it. These strategies were also used for study endophytic micro-organisms associated with this plant, since the metabolites present in plant tissues are often result of interaction between plants and different factors present in their habitat, such as micro-organisms. 50 strains of endophytic micro-organisms associated to Alternanthera brasiliana were isolated. High-Throughput Screening strategies, which envolved analytical techniques as LC-PDA-MS, LC-PDA-MS/MS, LC-SPE-NMR and antibiosis assays allowed identification of antibiotic compounds present in the A. brasiliana stems. These compounds are member of oxylipins group, among which we highlight the 13-Z,E-KODE, 13-E,E-KODE, 9-E,Z-KODE and 9-E,EKODE. Furthermore, were identified some depsipeptides compounds produced by a strain of Fusarium fungus, isolated as endophyte of A. brasiliana. Beauvericin, which is one of these compounds , is known for its antibiotic and insecticide activities. This compound was also found in the plant extracts, suggesting that endophyte microorganism could play a role in producing antibiotic compounds inside plant tissues. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-05-12 2016-06-02T20:36:31Z |
dc.date.issued.fl_str_mv |
2011-02-24 |
dc.date.accessioned.fl_str_mv |
2016-06-02T20:36:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TRAPP, Marília Almeida. Studies towards the dereplication of antibiotic constituents in Alternanthera brasiliana and associated microorganisms. 2011. 193 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/6490 |
identifier_str_mv |
TRAPP, Marília Almeida. Studies towards the dereplication of antibiotic constituents in Alternanthera brasiliana and associated microorganisms. 2011. 193 f. Dissertação (Mestrado em Ciências Exatas e da Terra) - Universidade Federal de São Carlos, São Carlos, 2011. |
url |
https://repositorio.ufscar.br/handle/ufscar/6490 |
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por |
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openAccess |
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Universidade Federal de São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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UFSCar |
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BR |
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Universidade Federal de São Carlos |
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