Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D

Detalhes bibliográficos
Autor(a) principal: Antunes, Bianca Sabino Leocádio
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/17368
Resumo: Bone Tissue Engineering (BOT) has been growing in recent decades as an effective approach for the treatment of large bone defects. Recently, three-dimensional (3D) printing has emerged as a tool to revolutionize the field of tissue engineering and regenerative medicine along with the release of osteogenic molecules such as Simvastatin (SIM). However, its immobilization in a framework/drug system is still a challenge. Silk fibroin (SF) has been shown to be a promising biomaterial for obtaining scaffolds due to its mechanical properties, controllable biodegradability and adequate cell adhesion/proliferation. The addition of other polymers is studied to control the viscosity of the SF solution, ensuring better quality of the printed structure. The objective of this work is to study the rheological properties of the gel based on SF and PLDLA (poly (L-co-D, L lactic acid)), with SIM, in order to obtain the best composition for 3D scaffold printing, aiming at the bone regeneration. PLDLA syntheses and SF were obtained from silk threads of the bombyx mori species. After this process, the pure SF and PLDLA samples were characterized by DSC, TG and FTIR. Then, SF-PLDLA solutions were prepared in formic acid, varying the concentrations of SF and PLDLA where they were subjected to rheological tests in permanent and oscillatory regimes. The chosen formulations were then submitted to the 3D printing process, obtaining structured scaffolds with pores, where the formulation of FS 15.0% with the addition of PLDLA 7.0%, resulted in the construction of a more reliable scaffold to the original design, compared to to the SF 15.0%/PLDLA 3.0% and SF 15.0%/PLDLA 10.0% formulations, presenting more evident pores and meeting the criteria of pseudoplasticity, viscoelasticity and thixotropy, important requirements for a solution to be suitable for 3D printing. The rheological assays showed that the addition of SIM at concentrations of 0.1%, 0.25%, 0.5%, 1.0% and 2.0% did not significantly alter the pseudoplastic, viscoelastic and thixotropic behavior of the gel SF 15.0%/PLDLA 7.0%.
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spelling Antunes, Bianca Sabino LeocádioDuek, Eliana Aparecida de Rezendehttp://lattes.cnpq.br/4147198882685212Komatsu, Danielhttp://lattes.cnpq.br/2620060426867863https://lattes.cnpq.br/1884620150270095ebb5eecb-ef00-47ad-bdcd-92b2f90bfbf92023-02-10T13:46:03Z2023-02-10T13:46:03Z2022-08-30ANTUNES, Bianca Sabino Leocádio. Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D. 2022. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/17368.https://repositorio.ufscar.br/handle/ufscar/17368Bone Tissue Engineering (BOT) has been growing in recent decades as an effective approach for the treatment of large bone defects. Recently, three-dimensional (3D) printing has emerged as a tool to revolutionize the field of tissue engineering and regenerative medicine along with the release of osteogenic molecules such as Simvastatin (SIM). However, its immobilization in a framework/drug system is still a challenge. Silk fibroin (SF) has been shown to be a promising biomaterial for obtaining scaffolds due to its mechanical properties, controllable biodegradability and adequate cell adhesion/proliferation. The addition of other polymers is studied to control the viscosity of the SF solution, ensuring better quality of the printed structure. The objective of this work is to study the rheological properties of the gel based on SF and PLDLA (poly (L-co-D, L lactic acid)), with SIM, in order to obtain the best composition for 3D scaffold printing, aiming at the bone regeneration. PLDLA syntheses and SF were obtained from silk threads of the bombyx mori species. After this process, the pure SF and PLDLA samples were characterized by DSC, TG and FTIR. Then, SF-PLDLA solutions were prepared in formic acid, varying the concentrations of SF and PLDLA where they were subjected to rheological tests in permanent and oscillatory regimes. The chosen formulations were then submitted to the 3D printing process, obtaining structured scaffolds with pores, where the formulation of FS 15.0% with the addition of PLDLA 7.0%, resulted in the construction of a more reliable scaffold to the original design, compared to to the SF 15.0%/PLDLA 3.0% and SF 15.0%/PLDLA 10.0% formulations, presenting more evident pores and meeting the criteria of pseudoplasticity, viscoelasticity and thixotropy, important requirements for a solution to be suitable for 3D printing. The rheological assays showed that the addition of SIM at concentrations of 0.1%, 0.25%, 0.5%, 1.0% and 2.0% did not significantly alter the pseudoplastic, viscoelastic and thixotropic behavior of the gel SF 15.0%/PLDLA 7.0%.A Engenharia de Tecidos Ósseos (ETO) vem crescendo nas últimas décadas como uma abordagem eficaz para o tratamento de grandes defeitos ósseos. Recentemente, a impressão tridimensional (3D) surgiu como uma ferramenta para revolucionar o campo da engenharia tecidual e medicina regenerativa junto a liberação de moléculas osteogênicas como a Sinvastatina (SIN). No entanto, sua imobilização em um sistema de arcabouço/fármacos ainda é um desafio. A fibroína da seda (FS) tem demonstrado ser um biomaterial promissor para a obtenção de arcabouços devido às suas propriedades mecânicas, biodegradabilidade controlável e adesão/proliferação celular adequada. A adição de outros polímeros é estudada para controlar a viscosidade da solução FS garantindo melhor qualidade da estrutura impressa. O objetivo deste trabalho é estudar as propriedades reológicas do gel à base de FS e PLDLA (poli (L-co-D, L ácido lático)), com SIN, a fim de obter a melhor composição para impressão do scaffold 3D, visando a regeneração óssea. Foram realizadas as sínteses do PLDLA e obtenção da FS a partir de fios de seda da espécie bombyx mori. Após esse processo, a amostra de FS pura e do PLDLA foram caracterizados por DSC, TG e FTIR. Em seguida foram preparadas soluções de FS-PLDLA, em ácido fórmico, variando as concentrações de FS e PLDLA onde foram submetidas aos ensaios reológicos em regimes permanente e oscilatório. As formulações escolhidas foram então submetidas ao processo de impressão 3D, obtendo scaffolds estruturados e com poros, onde a formulação de FS 15,0% com adição de PLDLA 7,0%, resultou na construção de um arcabouço mais fidedigno ao desenho original, comparado às formulações FS 15,0%/PLDLA 3,0% e FS 15,0%/PLDLA 10,0%, apresentando poros mais evidentes e atendendo aos critérios de pseudoplasticidade, viscoelasticidade e tixotropia, requisitos importantes para que uma solução seja apta à impressão 3D. Os ensaios reológicos mostraram que a adição de SIN, nas concentrações de 0,1%, 0,25%, 0,5% 1,0% e 2,0% não alteraram significativamente o comportamento pseudoplástico, viscoelástico e tixotrópico do gel FS 15,0%/PLDLA 7,0%.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)porUniversidade Federal de São CarlosCâmpus SorocabaPrograma de Pós-Graduação em Ciência dos Materiais - PPGCM-SoUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessReologiaHidrogelImpressão 3DPLDLAFibroína da SedaSinvastatinaRheologyHydrogel3D printingSilk FibroinSimvastatinENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA::MODELAGEM DE SISTEMAS BIOLOGICOSENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIAENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA::MATERIAIS NAO METALICOS::POLIMEROS, APLICACOESDesenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3DDevelopment and characterization of a bioactive gel based on silk fibroin/PLDLA/simvastatin for 3D printinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6006003b9cce8d-241d-4148-8382-c3247b1e0673reponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDISSERTAÇÃO mestrado.pdfDISSERTAÇÃO mestrado.pdfTese de mestradoapplication/pdf3550769https://repositorio.ufscar.br/bitstream/ufscar/17368/1/DISSERTA%c3%87%c3%83O%20mestrado.pdfd64bafee52cee777288eba3ec8804dc3MD51carta-comprovante_Bianca Antunes.pdfcarta-comprovante_Bianca Antunes.pdfCarta comprovanteapplication/pdf41342https://repositorio.ufscar.br/bitstream/ufscar/17368/2/carta-comprovante_Bianca%20Antunes.pdfca30781dd8439e31a3c3b9a9911c5cfcMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8810https://repositorio.ufscar.br/bitstream/ufscar/17368/3/license_rdff337d95da1fce0a22c77480e5e9a7aecMD53TEXTDISSERTAÇÃO mestrado.pdf.txtDISSERTAÇÃO mestrado.pdf.txtExtracted texttext/plain126510https://repositorio.ufscar.br/bitstream/ufscar/17368/4/DISSERTA%c3%87%c3%83O%20mestrado.pdf.txtb7cdc686f898984e9c7eb90b35bfa072MD54carta-comprovante_Bianca Antunes.pdf.txtcarta-comprovante_Bianca Antunes.pdf.txtExtracted texttext/plain1230https://repositorio.ufscar.br/bitstream/ufscar/17368/6/carta-comprovante_Bianca%20Antunes.pdf.txt0ca7a1d7795d4acee391c5941bc2bf75MD56THUMBNAILDISSERTAÇÃO mestrado.pdf.jpgDISSERTAÇÃO mestrado.pdf.jpgIM Thumbnailimage/jpeg9255https://repositorio.ufscar.br/bitstream/ufscar/17368/5/DISSERTA%c3%87%c3%83O%20mestrado.pdf.jpg099653b93405915073dd0fd7bb1e5dcdMD55carta-comprovante_Bianca Antunes.pdf.jpgcarta-comprovante_Bianca Antunes.pdf.jpgIM Thumbnailimage/jpeg6184https://repositorio.ufscar.br/bitstream/ufscar/17368/7/carta-comprovante_Bianca%20Antunes.pdf.jpg4b1924270ab7f601c3e5b475993218e0MD57ufscar/173682024-03-06 23:45:12.952oai:repositorio.ufscar.br:ufscar/17368Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222024-03-06T23:45:12Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
dc.title.alternative.eng.fl_str_mv Development and characterization of a bioactive gel based on silk fibroin/PLDLA/simvastatin for 3D printing
title Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
spellingShingle Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
Antunes, Bianca Sabino Leocádio
Reologia
Hidrogel
Impressão 3D
PLDLA
Fibroína da Seda
Sinvastatina
Rheology
Hydrogel
3D printing
Silk Fibroin
Simvastatin
ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA::MODELAGEM DE SISTEMAS BIOLOGICOS
ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA
ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA::MATERIAIS NAO METALICOS::POLIMEROS, APLICACOES
title_short Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
title_full Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
title_fullStr Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
title_full_unstemmed Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
title_sort Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D
author Antunes, Bianca Sabino Leocádio
author_facet Antunes, Bianca Sabino Leocádio
author_role author
dc.contributor.authorlattes.por.fl_str_mv https://lattes.cnpq.br/1884620150270095
dc.contributor.author.fl_str_mv Antunes, Bianca Sabino Leocádio
dc.contributor.advisor1.fl_str_mv Duek, Eliana Aparecida de Rezende
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4147198882685212
dc.contributor.advisor-co1.fl_str_mv Komatsu, Daniel
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2620060426867863
dc.contributor.authorID.fl_str_mv ebb5eecb-ef00-47ad-bdcd-92b2f90bfbf9
contributor_str_mv Duek, Eliana Aparecida de Rezende
Komatsu, Daniel
dc.subject.por.fl_str_mv Reologia
Hidrogel
Impressão 3D
PLDLA
Fibroína da Seda
Sinvastatina
topic Reologia
Hidrogel
Impressão 3D
PLDLA
Fibroína da Seda
Sinvastatina
Rheology
Hydrogel
3D printing
Silk Fibroin
Simvastatin
ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA::MODELAGEM DE SISTEMAS BIOLOGICOS
ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA
ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA::MATERIAIS NAO METALICOS::POLIMEROS, APLICACOES
dc.subject.eng.fl_str_mv Rheology
Hydrogel
3D printing
Silk Fibroin
Simvastatin
dc.subject.cnpq.fl_str_mv ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA::MODELAGEM DE SISTEMAS BIOLOGICOS
ENGENHARIAS::ENGENHARIA BIOMEDICA::BIOENGENHARIA
ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA::MATERIAIS NAO METALICOS::POLIMEROS, APLICACOES
description Bone Tissue Engineering (BOT) has been growing in recent decades as an effective approach for the treatment of large bone defects. Recently, three-dimensional (3D) printing has emerged as a tool to revolutionize the field of tissue engineering and regenerative medicine along with the release of osteogenic molecules such as Simvastatin (SIM). However, its immobilization in a framework/drug system is still a challenge. Silk fibroin (SF) has been shown to be a promising biomaterial for obtaining scaffolds due to its mechanical properties, controllable biodegradability and adequate cell adhesion/proliferation. The addition of other polymers is studied to control the viscosity of the SF solution, ensuring better quality of the printed structure. The objective of this work is to study the rheological properties of the gel based on SF and PLDLA (poly (L-co-D, L lactic acid)), with SIM, in order to obtain the best composition for 3D scaffold printing, aiming at the bone regeneration. PLDLA syntheses and SF were obtained from silk threads of the bombyx mori species. After this process, the pure SF and PLDLA samples were characterized by DSC, TG and FTIR. Then, SF-PLDLA solutions were prepared in formic acid, varying the concentrations of SF and PLDLA where they were subjected to rheological tests in permanent and oscillatory regimes. The chosen formulations were then submitted to the 3D printing process, obtaining structured scaffolds with pores, where the formulation of FS 15.0% with the addition of PLDLA 7.0%, resulted in the construction of a more reliable scaffold to the original design, compared to to the SF 15.0%/PLDLA 3.0% and SF 15.0%/PLDLA 10.0% formulations, presenting more evident pores and meeting the criteria of pseudoplasticity, viscoelasticity and thixotropy, important requirements for a solution to be suitable for 3D printing. The rheological assays showed that the addition of SIM at concentrations of 0.1%, 0.25%, 0.5%, 1.0% and 2.0% did not significantly alter the pseudoplastic, viscoelastic and thixotropic behavior of the gel SF 15.0%/PLDLA 7.0%.
publishDate 2022
dc.date.issued.fl_str_mv 2022-08-30
dc.date.accessioned.fl_str_mv 2023-02-10T13:46:03Z
dc.date.available.fl_str_mv 2023-02-10T13:46:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv ANTUNES, Bianca Sabino Leocádio. Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D. 2022. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/17368.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/17368
identifier_str_mv ANTUNES, Bianca Sabino Leocádio. Desenvolvimento e caracterização de gel bioativo à base de fibroína da seda/PLDLA/sinvastatina para impressão 3D. 2022. Dissertação (Mestrado em Ciência dos Materiais) – Universidade Federal de São Carlos, Sorocaba, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/17368.
url https://repositorio.ufscar.br/handle/ufscar/17368
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dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus Sorocaba
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