Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/7 |
Resumo: | ABSTRACT Microalgae include both algae Chlorophycea and cyanobacteria. These organisms have attracted interest due to its versatile metabolism, since some strains have, additionaly to photosynthetic metabolism, hability to grow in the dark by the consumption of simple organic molecules, such as glucose, acetate and glycerol. This hability suggests the application of these microorganisms in several areas, such as biological treatment of agroindustrial wastewater, aiming the removal of nutrients and organic matter. São Paulo/Brazil is one of the main producers of ethanol from sugarcane, generating a considerable amount of vinasse. Vinasse represents the main wastewater from de sugarcane agroindustry, being usually applied as fertigation in sugarcane crops. In this context, this study aimed to evaluate the heterotrophic growth of cyanobacterium Aphanothece microscopica Nägeli and the chlorophyceae Chorella vulgaris in synthetic culture media supplemented with organic carbon source and in vinasse, aiming to produce biomass and application it in wastewater treatment. Mixotrophic cultures were conducted to select the optimal concentration of organic substrate for the growth of microalgae, which were evaluated in heterotrophic cultures. Additionally, experiments have been conducted with pre-treated vinasse, evaluating the COD removal and the glucose and potassium incorporation. By the mixotrophic tests was selected the optimal concentrations of 25 and 12.5 g.L-1 glucose, 0.5 and 1.25 g.L-1 potassium acetate and 0.46 and 0.92 g.L-1 glycerol as ideal for the cultivation of Aphanothece microscopica Nägeli and Chlorella vulgaris, respectively, which reflected on the maximum specific growth rate between 0.0072 and 0.043 h-1. In the heterotrophic cultures the maximum specific growth rate was greater or equal to both microalgae in all carbon sources, with reduction between 30.4 and 90% of the initial concentrations of substrates. The experiments demonstrated possibility to use vinasse as culture media for both microalgae, especially Aphanothece microscopica Nägeli. In this conditions, results indicated the maximum removals of glucose (55.5%), COD (60.8%) and potassium (13%) by the cianobacterium, and 83.7% of glucose, 25% of COD and 13.8% of potassium by Chlorella vulgaris. The results suggest the heterotrophic metabolism for both microorganisms, pointing an interesting alternative to use of wastewater as culture media to produce high-value biomass from a low-cost way. |
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Bonini, Monica de AlbuquerqueBastos, Reinaldo Gasparhttp://lattes.cnpq.br/9329363278429665http://lattes.cnpq.br/549028733997929883a5e1c0-6a47-4d78-ac1d-9953b120e4862016-06-02T18:55:22Z2012-03-282016-06-02T18:55:22Z2012-03-08BONINI, Monica de Albuquerque. Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça. 2012. 112 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de São Carlos, São Carlos, 2012.https://repositorio.ufscar.br/handle/ufscar/7ABSTRACT Microalgae include both algae Chlorophycea and cyanobacteria. These organisms have attracted interest due to its versatile metabolism, since some strains have, additionaly to photosynthetic metabolism, hability to grow in the dark by the consumption of simple organic molecules, such as glucose, acetate and glycerol. This hability suggests the application of these microorganisms in several areas, such as biological treatment of agroindustrial wastewater, aiming the removal of nutrients and organic matter. São Paulo/Brazil is one of the main producers of ethanol from sugarcane, generating a considerable amount of vinasse. Vinasse represents the main wastewater from de sugarcane agroindustry, being usually applied as fertigation in sugarcane crops. In this context, this study aimed to evaluate the heterotrophic growth of cyanobacterium Aphanothece microscopica Nägeli and the chlorophyceae Chorella vulgaris in synthetic culture media supplemented with organic carbon source and in vinasse, aiming to produce biomass and application it in wastewater treatment. Mixotrophic cultures were conducted to select the optimal concentration of organic substrate for the growth of microalgae, which were evaluated in heterotrophic cultures. Additionally, experiments have been conducted with pre-treated vinasse, evaluating the COD removal and the glucose and potassium incorporation. By the mixotrophic tests was selected the optimal concentrations of 25 and 12.5 g.L-1 glucose, 0.5 and 1.25 g.L-1 potassium acetate and 0.46 and 0.92 g.L-1 glycerol as ideal for the cultivation of Aphanothece microscopica Nägeli and Chlorella vulgaris, respectively, which reflected on the maximum specific growth rate between 0.0072 and 0.043 h-1. In the heterotrophic cultures the maximum specific growth rate was greater or equal to both microalgae in all carbon sources, with reduction between 30.4 and 90% of the initial concentrations of substrates. The experiments demonstrated possibility to use vinasse as culture media for both microalgae, especially Aphanothece microscopica Nägeli. In this conditions, results indicated the maximum removals of glucose (55.5%), COD (60.8%) and potassium (13%) by the cianobacterium, and 83.7% of glucose, 25% of COD and 13.8% of potassium by Chlorella vulgaris. The results suggest the heterotrophic metabolism for both microorganisms, pointing an interesting alternative to use of wastewater as culture media to produce high-value biomass from a low-cost way.O termo microalga inclui tanto algas clorofíceas quanto cianobactérias. Recentemente estes micro-organismos têm despertado interesse devido ao seu metabolismo versátil, uma vez que algumas linhagens apresentam, além da fotossíntese como modelo de cultivo preferencial, a capacidade de desenvolverem-se no escuro a partir do consumo de moléculas orgânicas simples, tais como glicose, acetato e glicerol. Esta habilidade sugere a aplicação destes micro-organismos em diversas áreas, como o tratamento biológico de águas residuárias agroindustriais, visando a remoção de nutrientes e matéria orgânica. Apesar disso, são raros os trabalhos na literatura que avaliam o cultivo heterotrófico e o consumo ou incorporação de moléculas orgânicas por microalgas ou cianobactérias. O Estado de São Paulo responde por grande parte da produção nacional de etanol a partir da cana-de-açúcar, gerando uma quantidade considerável de vinhaça. A vinhaça é a principal água residuária do setor sucroenergético, sendo normalmente aplicada nos canaviais como fertirrigação. Neste contexto, o objetivo deste trabalho foi estender os estudos a respeito do cultivo mixotrófico e heterotrófico da cianofícea Aphanothece microscopica Nägeli e da clorofícea Chlorella vulgaris em meios com adição de fontes de carbono orgânico e em vinhaça, visando a produção de biomassa e aplicação no tratamento de águas residuárias. Ensaios mixotróficos foram conduzidos visando selecionar a concentração ideal de substrato orgânico para o cultivo das microalgas, as quais foram posteriormente avaliadas em sistemas heterotróficos. Paralelamente foram conduzidos ensaios com vinhaça, avaliando-se a remoção de DQO e incorporação de glicose e potássio pelas microalgas. Dos ensaios mixotróficos foram selecionadas as concentrações ótimas de 25 e 12,5 g.L-1 de glicose, 0,5 e 1,25 g.L-1 de acetato de potássio e 0,46 e 0,92 g.L-1 de glicerol como ideais para o cultivo de Aphanothece e Chlorella, respectivamente, refletindo em velocidades específicas de crescimento entre 0,0072 e 0,043 h-1. Nos ensaios heterotróficos verificaram-se velocidades específicas de crescimento iguais ou superiores para ambas as microalgas em todas as fontes de carbono avaliadas, com reduções entre 30,4 e 90% da concentração inicial dos substratos. Os ensaios com vinhaça demonstraram a possibilidade de utilização desta água residuária como meio de cultivo para ambas as microalgas, com alta conversão em biomassa pela Aphanothece. Nestas condições, verificaram-se remoções de 55,5% de glicose, 60,8% de DQO e 13% de potássio para a cianobactéria, e de 83,7% de glicose, 25% de DQO e 13,8% de potássio para Chlorella. Os resultados obtidos sugerem a presença do metabolismo heterotrófico nestes microorganismos e apontam uma alternativa interessante para o aproveitamento de águas residuárias, com a possibilidade de produzir biomassa de alto valor agregado a partir de um meio de baixo custo.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Agricultura e Ambiente - PPGAA-ArUFSCarBRBiotecnologiaCianobactériaMicroalgaCultivo heterotróficoÁguas residuaisVinhaçaAphanothece microscopica nägeliChlorella vulgarisAphanothece microscopica NägeliChlorella vulgarisHeterotrophic culturesWastewaterVinasseCIENCIAS AGRARIASCultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaçainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-1cb09db38-7017-4f6a-a8e0-d6de60933623info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL4151.pdfapplication/pdf927553https://repositorio.ufscar.br/bitstream/ufscar/7/1/4151.pdf064b20beb733cd6507b60f122733aee7MD51TEXT4151.pdf.txt4151.pdf.txtExtracted texttext/plain201502https://repositorio.ufscar.br/bitstream/ufscar/7/2/4151.pdf.txt5e236b750ec17a24e35e7786840cc91bMD52THUMBNAIL4151.pdf.jpg4151.pdf.jpgIM Thumbnailimage/jpeg5798https://repositorio.ufscar.br/bitstream/ufscar/7/3/4151.pdf.jpg0133f807c564a695b13cd17d6b598b0dMD53ufscar/72023-09-18 18:30:33.905oai:repositorio.ufscar.br:ufscar/7Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:33Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
title |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
spellingShingle |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça Bonini, Monica de Albuquerque Biotecnologia Cianobactéria Microalga Cultivo heterotrófico Águas residuais Vinhaça Aphanothece microscopica nägeli Chlorella vulgaris Aphanothece microscopica Nägeli Chlorella vulgaris Heterotrophic cultures Wastewater Vinasse CIENCIAS AGRARIAS |
title_short |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
title_full |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
title_fullStr |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
title_full_unstemmed |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
title_sort |
Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça |
author |
Bonini, Monica de Albuquerque |
author_facet |
Bonini, Monica de Albuquerque |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/5490287339979298 |
dc.contributor.author.fl_str_mv |
Bonini, Monica de Albuquerque |
dc.contributor.advisor1.fl_str_mv |
Bastos, Reinaldo Gaspar |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9329363278429665 |
dc.contributor.authorID.fl_str_mv |
83a5e1c0-6a47-4d78-ac1d-9953b120e486 |
contributor_str_mv |
Bastos, Reinaldo Gaspar |
dc.subject.por.fl_str_mv |
Biotecnologia Cianobactéria Microalga Cultivo heterotrófico Águas residuais Vinhaça Aphanothece microscopica nägeli Chlorella vulgaris |
topic |
Biotecnologia Cianobactéria Microalga Cultivo heterotrófico Águas residuais Vinhaça Aphanothece microscopica nägeli Chlorella vulgaris Aphanothece microscopica Nägeli Chlorella vulgaris Heterotrophic cultures Wastewater Vinasse CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Aphanothece microscopica Nägeli Chlorella vulgaris Heterotrophic cultures Wastewater Vinasse |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
ABSTRACT Microalgae include both algae Chlorophycea and cyanobacteria. These organisms have attracted interest due to its versatile metabolism, since some strains have, additionaly to photosynthetic metabolism, hability to grow in the dark by the consumption of simple organic molecules, such as glucose, acetate and glycerol. This hability suggests the application of these microorganisms in several areas, such as biological treatment of agroindustrial wastewater, aiming the removal of nutrients and organic matter. São Paulo/Brazil is one of the main producers of ethanol from sugarcane, generating a considerable amount of vinasse. Vinasse represents the main wastewater from de sugarcane agroindustry, being usually applied as fertigation in sugarcane crops. In this context, this study aimed to evaluate the heterotrophic growth of cyanobacterium Aphanothece microscopica Nägeli and the chlorophyceae Chorella vulgaris in synthetic culture media supplemented with organic carbon source and in vinasse, aiming to produce biomass and application it in wastewater treatment. Mixotrophic cultures were conducted to select the optimal concentration of organic substrate for the growth of microalgae, which were evaluated in heterotrophic cultures. Additionally, experiments have been conducted with pre-treated vinasse, evaluating the COD removal and the glucose and potassium incorporation. By the mixotrophic tests was selected the optimal concentrations of 25 and 12.5 g.L-1 glucose, 0.5 and 1.25 g.L-1 potassium acetate and 0.46 and 0.92 g.L-1 glycerol as ideal for the cultivation of Aphanothece microscopica Nägeli and Chlorella vulgaris, respectively, which reflected on the maximum specific growth rate between 0.0072 and 0.043 h-1. In the heterotrophic cultures the maximum specific growth rate was greater or equal to both microalgae in all carbon sources, with reduction between 30.4 and 90% of the initial concentrations of substrates. The experiments demonstrated possibility to use vinasse as culture media for both microalgae, especially Aphanothece microscopica Nägeli. In this conditions, results indicated the maximum removals of glucose (55.5%), COD (60.8%) and potassium (13%) by the cianobacterium, and 83.7% of glucose, 25% of COD and 13.8% of potassium by Chlorella vulgaris. The results suggest the heterotrophic metabolism for both microorganisms, pointing an interesting alternative to use of wastewater as culture media to produce high-value biomass from a low-cost way. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-03-28 2016-06-02T18:55:22Z |
dc.date.issued.fl_str_mv |
2012-03-08 |
dc.date.accessioned.fl_str_mv |
2016-06-02T18:55:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BONINI, Monica de Albuquerque. Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça. 2012. 112 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de São Carlos, São Carlos, 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/7 |
identifier_str_mv |
BONINI, Monica de Albuquerque. Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaça. 2012. 112 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de São Carlos, São Carlos, 2012. |
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https://repositorio.ufscar.br/handle/ufscar/7 |
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Universidade Federal de São Carlos |
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UFSCar |
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BR |
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