Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/8715 |
Resumo: | The titanium dioxide nanoparticles (NP-TiO2) has achieved into aquatic ecosystems through different sources, such as sewer and leaching. Abiotic factors such as pH, ionic strength, water hardness may change the chemical aggregation of NPs as well as the bioavailability of metals in waters result of speciation and /or complexation with other compounds. Considering the pH variations can occur in the environment naturally or as a result of ecological accidents and the increasing use of NP-TiO2 in many different industries, this study aimed to determine the CL-50; 48 hours of NPTiO2 in different pH (acidic, neutral and basic) in juvenile Prochilodus lineatus (curimbatá) and evaluating the effects of exposure to sublethal NP-TiO2 these pH using biochemical, physiological and morphological biomarkers. After sensitivity test with KCl, the animals were tested with different concentrations of NP-TiO2 at pH 5.0, 7.0 and 8.0 for 48 hours to determine the CL-50; 48h. Subsequently, the animals were exposed to 1 mg L-1 NP-TiO2 for 48 hours at different pH. After the exposure period, blood samples were obtained via caudal puncture for ion plasma analysis and hematologic variables, and samples of gills were collected and fixed. The analytical characterization of NP-TiO2 suggest the aggregation ability are modified of the NP at different pH and at pH 5.0, the hydrodynamic diameter of the NP is much larger then the others pH, which may have create difficults for the entry of NP in organisms and caused less damage. The NP-TiO2 were considered non-toxic in pH 5,0, 7,0 and 8,0. Exposure to NP-TiO2 pH 5.0 and ionic imbalance caused in the exposed animals, possibly due to changes in the concentration of Cl- ions. Exposure to pH 5.0 and 8.0 and NP-TiO2 at pH 7.0 inhibited AC enzyme. In animals exposed to NP at pH 5.0 were not identified cloride cells with crypt surface, possibly due the blocking of these surfaces by the large NP aggregates. Changes in concentrations of ROS and SOD, CAT and GCL activity may indicate an imbalance in antioxidant system due to different pH and NPTiO2 exposure. NP exposure caused major damage at pH 7.0, where have lower hydrodynamic diameter , supporting the hypothesis that the smaller the size of the cluster,it’s easier the NP enter the body of the exposed animals. |
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Souza, Naiara Elisabete da Silva deFernandes, Marisa Narcisohttp://lattes.cnpq.br/3021357484136942http://lattes.cnpq.br/83935049192057831c81baee-6d16-4a73-9869-6e64c9fa2f702017-05-04T12:21:59Z2017-05-04T12:21:59Z2015-10-08SOUZA, Naiara Elisabete da Silva de. Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8715.https://repositorio.ufscar.br/handle/ufscar/8715The titanium dioxide nanoparticles (NP-TiO2) has achieved into aquatic ecosystems through different sources, such as sewer and leaching. Abiotic factors such as pH, ionic strength, water hardness may change the chemical aggregation of NPs as well as the bioavailability of metals in waters result of speciation and /or complexation with other compounds. Considering the pH variations can occur in the environment naturally or as a result of ecological accidents and the increasing use of NP-TiO2 in many different industries, this study aimed to determine the CL-50; 48 hours of NPTiO2 in different pH (acidic, neutral and basic) in juvenile Prochilodus lineatus (curimbatá) and evaluating the effects of exposure to sublethal NP-TiO2 these pH using biochemical, physiological and morphological biomarkers. After sensitivity test with KCl, the animals were tested with different concentrations of NP-TiO2 at pH 5.0, 7.0 and 8.0 for 48 hours to determine the CL-50; 48h. Subsequently, the animals were exposed to 1 mg L-1 NP-TiO2 for 48 hours at different pH. After the exposure period, blood samples were obtained via caudal puncture for ion plasma analysis and hematologic variables, and samples of gills were collected and fixed. The analytical characterization of NP-TiO2 suggest the aggregation ability are modified of the NP at different pH and at pH 5.0, the hydrodynamic diameter of the NP is much larger then the others pH, which may have create difficults for the entry of NP in organisms and caused less damage. The NP-TiO2 were considered non-toxic in pH 5,0, 7,0 and 8,0. Exposure to NP-TiO2 pH 5.0 and ionic imbalance caused in the exposed animals, possibly due to changes in the concentration of Cl- ions. Exposure to pH 5.0 and 8.0 and NP-TiO2 at pH 7.0 inhibited AC enzyme. In animals exposed to NP at pH 5.0 were not identified cloride cells with crypt surface, possibly due the blocking of these surfaces by the large NP aggregates. Changes in concentrations of ROS and SOD, CAT and GCL activity may indicate an imbalance in antioxidant system due to different pH and NPTiO2 exposure. NP exposure caused major damage at pH 7.0, where have lower hydrodynamic diameter , supporting the hypothesis that the smaller the size of the cluster,it’s easier the NP enter the body of the exposed animals.As nanopartículas de dióxido de titânio (NP-TiO2) têm alcançado os ecossistemas aquáticos através de diferentes fontes, como esgotos e lixiviação das culturas agrícolas. Fatores abióticos como pH, força iônica, dureza da água podem alterar a agregação química das NPs e a biodisponibilidade de metais nas águas. Considerando as variações de pH que podem ocorrer no ambiente, naturalmente ou não, e a crescente utilização de NP-TiO2 nos mais diferentes segmentos industriais, o presente estudo teve por objetivo determinar a CL-50;48h das NP-TiO2 em diferentes pH (ácido, neutro e básico) em juvenis de Prochilodus lineatus (curimbatá) e avaliar os efeitos da exposição subletal a NP-TiO2 nesses pH, utilizando biomarcadores bioquímicos, fisiológicos e morfológicos. Após a realização do teste de sensibilidade com KCl, os animais foram submetidos a testes com diferentes concentrações de NP-TiO2 nos pH 5,0, 7,0 e 8,0 durante 48h para determinar a CL-50;48h. Posteriormente, os animais foram expostos à concentração de 1 mg L-1 de NP-TiO2 durante 48h nos diferentes pH. Após a exposição, amostras de sangue e de brânquias foram coletadas. Análises de caracterização das NP-TiO2 sugerem que em diferentes pH a capacidade de agregação das NP se modificam e que em pH 5,0, o diâmetro hidrodinâmico das NP é maior em relação aos outros pH, o que pode ter dificultado a entrada das NP nos organismos e causado danos menores. As NP-TiO2 foram consideradas não-tóxicas nos diferentes pH. A exposição a NP-TiO2 e a pH 5,0 causou desequilíbrio iônico nos animais expostos, possivelmente em função das alterações na concentração de Cl-. A exposição a pH 5,0 e 8,0 e a NP-TiO2 em pH 7,0 inibiu a enzima anidrase carbônica. Nos animais expostos a NP em pH 5,0 não foram identificadas CC em cripta, possivelmente devido ao bloqueio das superfícies dessas CC pelos grandes agregados de NP. As alterações nas concentrações de espécies reativas de oxigênio e da atividade das enzimas superóxido dismutase, catalase e glutamato cisteína ligase podem indicar um desequilíbrio no sistema antioxidante. A exposição as NP causou danos maiores em pH 7,0, onde os agregados apresentam diâmetro hidrodinâmico menor, corroborando com a hipótese de que quanto menor o tamanho dos aglomerado, maior a facilidade das NP entrarem no organismo dos animais expostos.OutraCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarNanopartícula de dióxido de titânioDiferentes pHBrânquiasProchilodus lineatusGillsTitanium dioxide nanoparticlesDifferent pHCIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionaisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline6006005470807a-1e63-4591-8511-0cb223b337f2info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissNESS.pdfDissNESS.pdfapplication/pdf3543889https://repositorio.ufscar.br/bitstream/ufscar/8715/1/DissNESS.pdf8aff5fb0c19f74c48e698260a94c29d2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/8715/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissNESS.pdf.txtDissNESS.pdf.txtExtracted texttext/plain193428https://repositorio.ufscar.br/bitstream/ufscar/8715/3/DissNESS.pdf.txtf1d0656f91b563a1b68c185683b568b7MD53THUMBNAILDissNESS.pdf.jpgDissNESS.pdf.jpgIM Thumbnailimage/jpeg6670https://repositorio.ufscar.br/bitstream/ufscar/8715/4/DissNESS.pdf.jpg00a8ec8efb368be2c9d94c08c1cf650dMD54ufscar/87152023-09-18 18:31:23.921oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:23Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
title |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
spellingShingle |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais Souza, Naiara Elisabete da Silva de Nanopartícula de dióxido de titânio Diferentes pH Brânquias Prochilodus lineatus Gills Titanium dioxide nanoparticles Different pH CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
title_full |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
title_fullStr |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
title_full_unstemmed |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
title_sort |
Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais |
author |
Souza, Naiara Elisabete da Silva de |
author_facet |
Souza, Naiara Elisabete da Silva de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/8393504919205783 |
dc.contributor.author.fl_str_mv |
Souza, Naiara Elisabete da Silva de |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Marisa Narciso |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3021357484136942 |
dc.contributor.authorID.fl_str_mv |
1c81baee-6d16-4a73-9869-6e64c9fa2f70 |
contributor_str_mv |
Fernandes, Marisa Narciso |
dc.subject.por.fl_str_mv |
Nanopartícula de dióxido de titânio Diferentes pH Brânquias |
topic |
Nanopartícula de dióxido de titânio Diferentes pH Brânquias Prochilodus lineatus Gills Titanium dioxide nanoparticles Different pH CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.lat.fl_str_mv |
Prochilodus lineatus |
dc.subject.eng.fl_str_mv |
Gills Titanium dioxide nanoparticles Different pH |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
The titanium dioxide nanoparticles (NP-TiO2) has achieved into aquatic ecosystems through different sources, such as sewer and leaching. Abiotic factors such as pH, ionic strength, water hardness may change the chemical aggregation of NPs as well as the bioavailability of metals in waters result of speciation and /or complexation with other compounds. Considering the pH variations can occur in the environment naturally or as a result of ecological accidents and the increasing use of NP-TiO2 in many different industries, this study aimed to determine the CL-50; 48 hours of NPTiO2 in different pH (acidic, neutral and basic) in juvenile Prochilodus lineatus (curimbatá) and evaluating the effects of exposure to sublethal NP-TiO2 these pH using biochemical, physiological and morphological biomarkers. After sensitivity test with KCl, the animals were tested with different concentrations of NP-TiO2 at pH 5.0, 7.0 and 8.0 for 48 hours to determine the CL-50; 48h. Subsequently, the animals were exposed to 1 mg L-1 NP-TiO2 for 48 hours at different pH. After the exposure period, blood samples were obtained via caudal puncture for ion plasma analysis and hematologic variables, and samples of gills were collected and fixed. The analytical characterization of NP-TiO2 suggest the aggregation ability are modified of the NP at different pH and at pH 5.0, the hydrodynamic diameter of the NP is much larger then the others pH, which may have create difficults for the entry of NP in organisms and caused less damage. The NP-TiO2 were considered non-toxic in pH 5,0, 7,0 and 8,0. Exposure to NP-TiO2 pH 5.0 and ionic imbalance caused in the exposed animals, possibly due to changes in the concentration of Cl- ions. Exposure to pH 5.0 and 8.0 and NP-TiO2 at pH 7.0 inhibited AC enzyme. In animals exposed to NP at pH 5.0 were not identified cloride cells with crypt surface, possibly due the blocking of these surfaces by the large NP aggregates. Changes in concentrations of ROS and SOD, CAT and GCL activity may indicate an imbalance in antioxidant system due to different pH and NPTiO2 exposure. NP exposure caused major damage at pH 7.0, where have lower hydrodynamic diameter , supporting the hypothesis that the smaller the size of the cluster,it’s easier the NP enter the body of the exposed animals. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-10-08 |
dc.date.accessioned.fl_str_mv |
2017-05-04T12:21:59Z |
dc.date.available.fl_str_mv |
2017-05-04T12:21:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA, Naiara Elisabete da Silva de. Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8715. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/8715 |
identifier_str_mv |
SOUZA, Naiara Elisabete da Silva de. Efeitos da exposição à nanopartícula de dióxido de titânio em diferentes pH do meio aquático em brânquias de curimbatá (Prochilodus lineatus, Teleostei, Prochilodontidae) : aspectos bioquímicos, fisiológicos e morfofuncionais. 2015. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2015. Disponível em: https://repositorio.ufscar.br/handle/ufscar/8715. |
url |
https://repositorio.ufscar.br/handle/ufscar/8715 |
dc.language.iso.fl_str_mv |
por |
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por |
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600 600 |
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5470807a-1e63-4591-8511-0cb223b337f2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
dc.publisher.initials.fl_str_mv |
UFSCar |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.source.none.fl_str_mv |
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institution |
UFSCAR |
reponame_str |
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collection |
Repositório Institucional da UFSCAR |
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