Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/5255 |
Resumo: | Osteoporosis is generally a systematic skeletal disease characterized by low bone density and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Osteopenia is characterised by a lower bone density than normal bone tissue but higher than osteoporotic bone tissue. Osteoporosis and osteopenia represent a severe health threat to elderly people and they have recently been recognised as a major public health problem. They are related with some clinic manifestations, mainly the increase of the fractures risk. In this context, there is a critical need to develop technologies able of treating osteoporotic and osteopenic fractures. Promising treatments are the use of biomaterials and the Low Level Laser Therapy (LLLT), which seem to induce osteogenesis and stimulate fracture healing. The aim of this study was to determine the effects of the Biosilicate® and the LLLT on bone consolidation of osteopenic rat. Seventy female Wistar rats (12 weeks-old, ± 250g) ovariectomy (OVX) was carried on. The animals were randomly divided into 7 groups, with 10 animals each: standard control (CP); defect bone control (CD), bone defect treated with Biosilicate® (B), bone defect treated with laser 60J/cm2 (L60), bone defect treated with laser 120J/cm2 (L120), bone defect treated with both treatments Biosilicate® and laser 60J/cm2 (B+L60), bone defect treated with both treatments Biosilicate® and laser 120J/cm2 (B+L120). Sixty days post-OVX the osteotomies were surgically performed on the left tibia. In the Biosilicate® treated animals, the cavities were carefully filled with the biomaterial. An 830nm laser was performed for seven sessions. On day 14 post-osteotomy, rats were sacrificed and the tibias were defleshed. Biomechanical properties of the tibia were determined by two tests: the Indentation Test to a depth of 0-0.5mm, 0-1.0mm and 0-1.5mm and the Three-Point Bending Test. From de load-deformation curve, the maximal load (KN) and energy absorption (J) were obtained. Statistical analyses were performed using Kruskal-Wallis Test and Mann-Whitney U Test, with the level of significance of 5% (p≤0.05). In relation to biomechanical properties of the Indentation Test, the groups CP, B, B+L60 and B+L120 presented higher statistically values (p<0.05) in relation to group CD. The better biomechanical answer among the groups was present by the group with Biosilicate® utilization in association of 120J/cm². In relation to maximal load of the Three-Point Bending Test, the group L60 presented higher statistically values (p< 0.05) in relation to group CP, B, L120, B+L60, B+L120 and the groups had similar values of absorption energy. The Biosilicate® application raised the biomechanical properties of the callus bone, but didn t raise the biomechanical properties of the tibia determined by Three-Point Bending Test. In the two biomechanical tests, the groups with treatment based only on laser irradiation haven t presented significant results in relation to group control fracture. The bones defects treated with both treatments Biosilicate® and laser 120J/cm2 presented higher biomechanical properties of the callus bone in relation to the group based only Biosilicate® application. |
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Fangel, RenanOishi, Jorgehttp://lattes.cnpq.br/1417328590472858http://lattes.cnpq.br/1973857855614173301122e6-e6bb-46bd-b744-9f1fe0e50b1f2016-06-02T20:19:12Z2009-11-302016-06-02T20:19:12Z2009-08-07FANGEL, Renan. Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas. 2009. 137 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009.https://repositorio.ufscar.br/handle/ufscar/5255Osteoporosis is generally a systematic skeletal disease characterized by low bone density and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Osteopenia is characterised by a lower bone density than normal bone tissue but higher than osteoporotic bone tissue. Osteoporosis and osteopenia represent a severe health threat to elderly people and they have recently been recognised as a major public health problem. They are related with some clinic manifestations, mainly the increase of the fractures risk. In this context, there is a critical need to develop technologies able of treating osteoporotic and osteopenic fractures. Promising treatments are the use of biomaterials and the Low Level Laser Therapy (LLLT), which seem to induce osteogenesis and stimulate fracture healing. The aim of this study was to determine the effects of the Biosilicate® and the LLLT on bone consolidation of osteopenic rat. Seventy female Wistar rats (12 weeks-old, ± 250g) ovariectomy (OVX) was carried on. The animals were randomly divided into 7 groups, with 10 animals each: standard control (CP); defect bone control (CD), bone defect treated with Biosilicate® (B), bone defect treated with laser 60J/cm2 (L60), bone defect treated with laser 120J/cm2 (L120), bone defect treated with both treatments Biosilicate® and laser 60J/cm2 (B+L60), bone defect treated with both treatments Biosilicate® and laser 120J/cm2 (B+L120). Sixty days post-OVX the osteotomies were surgically performed on the left tibia. In the Biosilicate® treated animals, the cavities were carefully filled with the biomaterial. An 830nm laser was performed for seven sessions. On day 14 post-osteotomy, rats were sacrificed and the tibias were defleshed. Biomechanical properties of the tibia were determined by two tests: the Indentation Test to a depth of 0-0.5mm, 0-1.0mm and 0-1.5mm and the Three-Point Bending Test. From de load-deformation curve, the maximal load (KN) and energy absorption (J) were obtained. Statistical analyses were performed using Kruskal-Wallis Test and Mann-Whitney U Test, with the level of significance of 5% (p≤0.05). In relation to biomechanical properties of the Indentation Test, the groups CP, B, B+L60 and B+L120 presented higher statistically values (p<0.05) in relation to group CD. The better biomechanical answer among the groups was present by the group with Biosilicate® utilization in association of 120J/cm². In relation to maximal load of the Three-Point Bending Test, the group L60 presented higher statistically values (p< 0.05) in relation to group CP, B, L120, B+L60, B+L120 and the groups had similar values of absorption energy. The Biosilicate® application raised the biomechanical properties of the callus bone, but didn t raise the biomechanical properties of the tibia determined by Three-Point Bending Test. In the two biomechanical tests, the groups with treatment based only on laser irradiation haven t presented significant results in relation to group control fracture. The bones defects treated with both treatments Biosilicate® and laser 120J/cm2 presented higher biomechanical properties of the callus bone in relation to the group based only Biosilicate® application.A osteoporose é uma doença esquelética sistêmica caracterizada por baixa densidade óssea e deterioração da microarquitetura do tecido ósseo com conseqüente aumento da fragilidade óssea. A osteopenia é caracterizada por apresentar uma menor densidade óssea que o tecido ósseo normal, mas maiores valores do que o tecido ósseo osteoporótico. A osteoporose e a osteopenia representam um grave problema de saúde a idosos e recentemente têm sido reconhecidos como um dos principais problemas de saúde pública, principalmente por aumentar o risco de fraturas. Neste contexto, há uma grande necessidade de se desenvolver tecnologias capazes de tratar fraturas em organismos osteoporóticos e osteopênicos. Existem diversos tratamentos promissores, como o uso do Biosilicato® e da terapia laser, os quais parecem induzir a osteogênese e estimulam o reparo ósseo. O objetivo deste estudo foi determinar os efeitos do o Biosilicato® e da terapia laser sobre o reparo ósseo de ratas osteopênicas. Setenta ratas fêmeas da raça Wistar (12-semanas, ± 250g) foram ovariectomizadas e divididas em sete grupos com 10 animais em cada: controle padrão (CP), controle com defeito ósseo (CD), tratado com Biosilicato® (B), tratado com 60J/cm2 (L60), tratado com 120J/cm2 (L120), tratado com Biosilicato® e 60J/cm2 (B+L60), tratado com Biosilicato® e 120J/cm2 (B+L120). Sessenta dias após a ovariectomia, as tíbias foram osteotomizadas. Nos animais tratados com Biosilicato®, a cavidade do defeito ósseo foi preenchida com o biomaterial. Os animais foram irradiados com 830nm por sete sessões (48- 48h). No décimo quarto dia após a osteotomia, as ratas foram sacrificadas e as tíbias dessecadas. As propriedades biomecânicas das tíbias esquerdas foram determinadas por dois testes: O Teste de Endentação com profundidade de 0-0,5mm, 0-1,0mm e 0-1,5mm que avaliou as propriedades biomecânicas do calo ósseo e o Teste de Flexão a Três Pontos que avaliou as propriedades biomecânicas das regiões ósseas integras do osso osteotomizado, sem considerar a região do calo ósseo que foi utilizada no teste de Endentação. A carga máxima (KN) e a energia de absorção (J) foram obtidas da curva de carga-deslocamento. A análise estatística foi realizada pelo Teste de Kruskal-Wallis e o Teste de Mann-Whitney U, com p≤0,05. Em relação ao Teste de Endentação, os grupos CP, B, B+L60 e B+L120 apresentaram maiores valores estatísticos (p<0,05) em relação ao grupo CD. A melhor resposta biomecânica do Teste de Endentação foi apresentada pelo grupo B+L120. Em relação à carga máxima do Teste de Flexão a Três Pontos, o grupo L60 apresentou maiores valores estatísticos (p<0,05) do que os grupos CP, B, L120, B+L60, B+L120 e todos os grupos tiveram valores semelhantes de energia de absorção. Pode-se concluir que a aplicação do Biosilicato® aumentou as propriedades biomecânicas do calo ósseo e não alterou as propriedades das regiões ósseas integras. A terapia laser não promoveu diferença significativa das propriedades biomecânicas do calo ósseo e das regiões ósseas integras e a associação dos dois tratamentos com fluência de 120J/cm² promoveu um aumento das propriedades biomecânicas do calo ósseo a valores mais elevados do que o grupo que utilizou somente o biomaterial.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Fisioterapia - PPGFtUFSCarBRFisioterapiaFototerapiaBiomateriaisReparo ósseoTerapia laser de baixa intensidadePropriedades biomecânicasFracture healingBiomaterialLow level laser therapyBiomechanical propertiesCIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONALOs efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-18517977a-64de-48a3-b3d4-2a0510270457info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL2618.pdfapplication/pdf1011844https://repositorio.ufscar.br/bitstream/ufscar/5255/1/2618.pdfacc696873b3edd56b2bb4801a43ed7e8MD51THUMBNAIL2618.pdf.jpg2618.pdf.jpgIM Thumbnailimage/jpeg7834https://repositorio.ufscar.br/bitstream/ufscar/5255/2/2618.pdf.jpg5865661af79946543a849295e502f998MD52ufscar/52552023-09-18 18:31:06.085oai:repositorio.ufscar.br:ufscar/5255Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:06Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
title |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
spellingShingle |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas Fangel, Renan Fisioterapia Fototerapia Biomateriais Reparo ósseo Terapia laser de baixa intensidade Propriedades biomecânicas Fracture healing Biomaterial Low level laser therapy Biomechanical properties CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL |
title_short |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
title_full |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
title_fullStr |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
title_full_unstemmed |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
title_sort |
Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas |
author |
Fangel, Renan |
author_facet |
Fangel, Renan |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/1973857855614173 |
dc.contributor.author.fl_str_mv |
Fangel, Renan |
dc.contributor.advisor1.fl_str_mv |
Oishi, Jorge |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1417328590472858 |
dc.contributor.authorID.fl_str_mv |
301122e6-e6bb-46bd-b744-9f1fe0e50b1f |
contributor_str_mv |
Oishi, Jorge |
dc.subject.por.fl_str_mv |
Fisioterapia Fototerapia Biomateriais Reparo ósseo Terapia laser de baixa intensidade Propriedades biomecânicas |
topic |
Fisioterapia Fototerapia Biomateriais Reparo ósseo Terapia laser de baixa intensidade Propriedades biomecânicas Fracture healing Biomaterial Low level laser therapy Biomechanical properties CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL |
dc.subject.eng.fl_str_mv |
Fracture healing Biomaterial Low level laser therapy Biomechanical properties |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL |
description |
Osteoporosis is generally a systematic skeletal disease characterized by low bone density and microarchitectural deterioration of bone tissue with a consequent increase in bone fragility. Osteopenia is characterised by a lower bone density than normal bone tissue but higher than osteoporotic bone tissue. Osteoporosis and osteopenia represent a severe health threat to elderly people and they have recently been recognised as a major public health problem. They are related with some clinic manifestations, mainly the increase of the fractures risk. In this context, there is a critical need to develop technologies able of treating osteoporotic and osteopenic fractures. Promising treatments are the use of biomaterials and the Low Level Laser Therapy (LLLT), which seem to induce osteogenesis and stimulate fracture healing. The aim of this study was to determine the effects of the Biosilicate® and the LLLT on bone consolidation of osteopenic rat. Seventy female Wistar rats (12 weeks-old, ± 250g) ovariectomy (OVX) was carried on. The animals were randomly divided into 7 groups, with 10 animals each: standard control (CP); defect bone control (CD), bone defect treated with Biosilicate® (B), bone defect treated with laser 60J/cm2 (L60), bone defect treated with laser 120J/cm2 (L120), bone defect treated with both treatments Biosilicate® and laser 60J/cm2 (B+L60), bone defect treated with both treatments Biosilicate® and laser 120J/cm2 (B+L120). Sixty days post-OVX the osteotomies were surgically performed on the left tibia. In the Biosilicate® treated animals, the cavities were carefully filled with the biomaterial. An 830nm laser was performed for seven sessions. On day 14 post-osteotomy, rats were sacrificed and the tibias were defleshed. Biomechanical properties of the tibia were determined by two tests: the Indentation Test to a depth of 0-0.5mm, 0-1.0mm and 0-1.5mm and the Three-Point Bending Test. From de load-deformation curve, the maximal load (KN) and energy absorption (J) were obtained. Statistical analyses were performed using Kruskal-Wallis Test and Mann-Whitney U Test, with the level of significance of 5% (p≤0.05). In relation to biomechanical properties of the Indentation Test, the groups CP, B, B+L60 and B+L120 presented higher statistically values (p<0.05) in relation to group CD. The better biomechanical answer among the groups was present by the group with Biosilicate® utilization in association of 120J/cm². In relation to maximal load of the Three-Point Bending Test, the group L60 presented higher statistically values (p< 0.05) in relation to group CP, B, L120, B+L60, B+L120 and the groups had similar values of absorption energy. The Biosilicate® application raised the biomechanical properties of the callus bone, but didn t raise the biomechanical properties of the tibia determined by Three-Point Bending Test. In the two biomechanical tests, the groups with treatment based only on laser irradiation haven t presented significant results in relation to group control fracture. The bones defects treated with both treatments Biosilicate® and laser 120J/cm2 presented higher biomechanical properties of the callus bone in relation to the group based only Biosilicate® application. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-11-30 2016-06-02T20:19:12Z |
dc.date.issued.fl_str_mv |
2009-08-07 |
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2016-06-02T20:19:12Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FANGEL, Renan. Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas. 2009. 137 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/5255 |
identifier_str_mv |
FANGEL, Renan. Os efeitos do laser de baixa intensidade e do Biosilicato®, utilizados independentemente ou associados, sobre o reparo ósseo em ratas osteopênicas. 2009. 137 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009. |
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https://repositorio.ufscar.br/handle/ufscar/5255 |
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Universidade Federal de São Carlos |
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UFSCar |
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BR |
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