Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/15967 |
Resumo: | Citriculture has been present in Brazil since its colonization when orange seedlings were imported and commercialized. Due to the fertile soil and favorable climate, over the decades, Brazil has become the largest producer of oranges and exporter of orange juice worldwide, generating a lot of profit for the country. Nevertheless, over time, several diseases that bring significant losses to the citrus industry have also developed, and many of them still have no cure. Citrus canker is one of them and is caused by the bacterium Xanthomonas citri subsp. citri (Xac). Many investigations have been carried out to elucidate the infection mechanism of the bacteria in the host, how they defend themselves and why they are so virulent. After a proteomic analysis of the cell surface performed on Xac under two different conditions, host infection and non-infection, different amounts of some proteins on the surface were found, being many of them already reported and related to virulence in other bacteria and classified as moonlighting proteins, which are proteins that have a well-defined function usually in the cytosol, but were also found performing completely different functions in other cellular regions, mostly on the surface. Such proteins may be related to biofilm production, defense response against the host, adhesion of bacteria to surfaces, and various other functions. Studies in recent years have tried to unravel their possible recognition mechanisms inside the cell and how they are secreted out, as well as trying to understand what functions they have when they are on the surface. In this work, the topics mentioned above were addressed in detail and the possible relationship of surface moonlighting proteins with the virulence of the citrus canker-causing bacteria was discussed. |
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Ferreira, Jéssica CamilaSouza, Dulce Helena Ferreira dehttp://lattes.cnpq.br/34289552995260032f5dd9b9-fd1a-457c-908f-9b8feec8f7d72022-05-03T10:54:31Z2022-05-03T10:54:31Z2022-04-11FERREIRA, Jéssica Camila. Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico. 2022. Trabalho de Conclusão de Curso (Graduação em Química) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15967.https://repositorio.ufscar.br/handle/ufscar/15967Citriculture has been present in Brazil since its colonization when orange seedlings were imported and commercialized. Due to the fertile soil and favorable climate, over the decades, Brazil has become the largest producer of oranges and exporter of orange juice worldwide, generating a lot of profit for the country. Nevertheless, over time, several diseases that bring significant losses to the citrus industry have also developed, and many of them still have no cure. Citrus canker is one of them and is caused by the bacterium Xanthomonas citri subsp. citri (Xac). Many investigations have been carried out to elucidate the infection mechanism of the bacteria in the host, how they defend themselves and why they are so virulent. After a proteomic analysis of the cell surface performed on Xac under two different conditions, host infection and non-infection, different amounts of some proteins on the surface were found, being many of them already reported and related to virulence in other bacteria and classified as moonlighting proteins, which are proteins that have a well-defined function usually in the cytosol, but were also found performing completely different functions in other cellular regions, mostly on the surface. Such proteins may be related to biofilm production, defense response against the host, adhesion of bacteria to surfaces, and various other functions. Studies in recent years have tried to unravel their possible recognition mechanisms inside the cell and how they are secreted out, as well as trying to understand what functions they have when they are on the surface. In this work, the topics mentioned above were addressed in detail and the possible relationship of surface moonlighting proteins with the virulence of the citrus canker-causing bacteria was discussed.A citricultura está presente no Brasil desde sua colonização, quando mudas de laranja foram trazidas e comercializadas. Devido ao solo fértil e clima favorável, ao longo das décadas, o Brasil se tornou o maior produtor de laranjas e exportador de suco de laranja do mundo, gerando muito lucro para o país. Porém, com o passar do tempo também se desenvolveu diversas doenças que trazem grandes prejuízos para a citricultura e muitas delas ainda não têm cura. O cancro cítrico é uma delas e é causado pela bactéria Xanthomonas citri subsp. citri (Xac). Muitas pesquisas têm sido realizadas a fim de elucidar o mecanismo de infecção da bactéria no hospedeiro, como elas fazem para se defender e porque são tão virulentas. Após uma análise proteômica da superfície celular realizada em Xac em duas condições diferentes, de infecção no hospedeiro e não infecção, foram constatadas diferentes quantidades de algumas proteínas na superfície, onde muitas delas já haviam sido relatadas e relacionadas com a virulência em outras bactérias e foram classificadas como proteínas moonlighting, que são proteínas que possuem uma função bem definida, geralmente no citosol, mas que também foram encontradas desempenhando funções completamente diferentes em outras regiões celulares, na maioria das vezes na superfície. Tais proteínas podem estar relacionadas com a produção de biofilme, resposta de defesa contra o hospedeiro, adesão das bactérias nas superfícies e diversas outras funções. Estudos realizados nos últimos anos tentaram desvendar seus possíveis mecanismos de reconhecimento dentro da célula e como são secretadas para fora, além de tentar entender quais funções elas possuem quando estão na superfície. Neste trabalho, os tópicos citados acima foram abordados de forma detalhada e discutiu-se a possível relação das proteínas moonlighting encontradas superficialmente com a virulência da bactéria causadora do cancro cítrico.Não recebi financiamentoporUniversidade Federal de São CarlosCâmpus São CarlosQuímica - QUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessProteínas moonlightingCancro cítricoMoonlighting proteinsCitrus cankerXanthomonas citriCIENCIAS BIOLOGICAS::BIOQUIMICA::QUIMICA DE MACROMOLECULAS::PROTEINASProteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítricoMoonlighting proteins and their relationship with bacterial pathogenicity Xanthomonas citri subsp. citri, which causes citrus cankerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis600600d64acd2a-121f-4d93-b884-274d4b53bf99reponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALTCC Jéssica Camila Ferreira VF 27042022.pdfTCC Jéssica Camila Ferreira VF 27042022.pdfTCCapplication/pdf824145https://repositorio.ufscar.br/bitstream/ufscar/15967/1/TCC%20J%c3%a9ssica%20Camila%20Ferreira%20VF%2027042022.pdf24e7dad30a8cba6261ea5fe87c3c2443MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15967/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52TEXTTCC Jéssica Camila Ferreira VF 27042022.pdf.txtTCC Jéssica Camila Ferreira VF 27042022.pdf.txtExtracted texttext/plain52205https://repositorio.ufscar.br/bitstream/ufscar/15967/3/TCC%20J%c3%a9ssica%20Camila%20Ferreira%20VF%2027042022.pdf.txt9fd762d35eab3c649ed452612a28dfd7MD53THUMBNAILTCC Jéssica Camila Ferreira VF 27042022.pdf.jpgTCC Jéssica Camila Ferreira VF 27042022.pdf.jpgIM Thumbnailimage/jpeg6034https://repositorio.ufscar.br/bitstream/ufscar/15967/4/TCC%20J%c3%a9ssica%20Camila%20Ferreira%20VF%2027042022.pdf.jpg369a45ca5d6399244c737554b5891a8dMD54ufscar/159672023-09-18 18:32:24.216oai:repositorio.ufscar.br:ufscar/15967Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:24Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
dc.title.alternative.eng.fl_str_mv |
Moonlighting proteins and their relationship with bacterial pathogenicity Xanthomonas citri subsp. citri, which causes citrus canker |
title |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
spellingShingle |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico Ferreira, Jéssica Camila Proteínas moonlighting Cancro cítrico Moonlighting proteins Citrus canker Xanthomonas citri CIENCIAS BIOLOGICAS::BIOQUIMICA::QUIMICA DE MACROMOLECULAS::PROTEINAS |
title_short |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
title_full |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
title_fullStr |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
title_full_unstemmed |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
title_sort |
Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico |
author |
Ferreira, Jéssica Camila |
author_facet |
Ferreira, Jéssica Camila |
author_role |
author |
dc.contributor.author.fl_str_mv |
Ferreira, Jéssica Camila |
dc.contributor.advisor1.fl_str_mv |
Souza, Dulce Helena Ferreira de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3428955299526003 |
dc.contributor.authorID.fl_str_mv |
2f5dd9b9-fd1a-457c-908f-9b8feec8f7d7 |
contributor_str_mv |
Souza, Dulce Helena Ferreira de |
dc.subject.por.fl_str_mv |
Proteínas moonlighting Cancro cítrico |
topic |
Proteínas moonlighting Cancro cítrico Moonlighting proteins Citrus canker Xanthomonas citri CIENCIAS BIOLOGICAS::BIOQUIMICA::QUIMICA DE MACROMOLECULAS::PROTEINAS |
dc.subject.eng.fl_str_mv |
Moonlighting proteins Citrus canker Xanthomonas citri |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOQUIMICA::QUIMICA DE MACROMOLECULAS::PROTEINAS |
description |
Citriculture has been present in Brazil since its colonization when orange seedlings were imported and commercialized. Due to the fertile soil and favorable climate, over the decades, Brazil has become the largest producer of oranges and exporter of orange juice worldwide, generating a lot of profit for the country. Nevertheless, over time, several diseases that bring significant losses to the citrus industry have also developed, and many of them still have no cure. Citrus canker is one of them and is caused by the bacterium Xanthomonas citri subsp. citri (Xac). Many investigations have been carried out to elucidate the infection mechanism of the bacteria in the host, how they defend themselves and why they are so virulent. After a proteomic analysis of the cell surface performed on Xac under two different conditions, host infection and non-infection, different amounts of some proteins on the surface were found, being many of them already reported and related to virulence in other bacteria and classified as moonlighting proteins, which are proteins that have a well-defined function usually in the cytosol, but were also found performing completely different functions in other cellular regions, mostly on the surface. Such proteins may be related to biofilm production, defense response against the host, adhesion of bacteria to surfaces, and various other functions. Studies in recent years have tried to unravel their possible recognition mechanisms inside the cell and how they are secreted out, as well as trying to understand what functions they have when they are on the surface. In this work, the topics mentioned above were addressed in detail and the possible relationship of surface moonlighting proteins with the virulence of the citrus canker-causing bacteria was discussed. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-03T10:54:31Z |
dc.date.available.fl_str_mv |
2022-05-03T10:54:31Z |
dc.date.issued.fl_str_mv |
2022-04-11 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/bachelorThesis |
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FERREIRA, Jéssica Camila. Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico. 2022. Trabalho de Conclusão de Curso (Graduação em Química) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15967. |
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https://repositorio.ufscar.br/handle/ufscar/15967 |
identifier_str_mv |
FERREIRA, Jéssica Camila. Proteínas moonlighting e sua relação com a patogenicidade da bactéria Xanthomonas citri subsp. citri, causadora do cancro cítrico. 2022. Trabalho de Conclusão de Curso (Graduação em Química) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15967. |
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https://repositorio.ufscar.br/handle/ufscar/15967 |
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