Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/1289 |
Resumo: | The ability to sense and respond to oxygen availability in the environment is of extreme functional importance for fish. This is even more so in tropical freshwater habitats where environmental hypoxia can occur rapidly in space and time. Recent physiological studies on the cardio-respiratory response of tropical fishes have focused on hypoxia tolerant species. This aim of this study was to examine the neural control and function of the cardio-respiratory system in a fish that is adapted to living at high levels of oxygen. Trairão, Hoplias lacerdae was chosen because it is a member of the Erythrinidae family, in which most of species are hypoxia tolerant, including some where the O2 receptors has already been characterized. The study focused on investigation of the localization, distribution and orientation of chemoreceptors of O2 in trairão. Also how the cardio-respiratory parameters were modified by hypoxia. The fish were submitted to selective denervations of the branchial branches of the cranial nerves IX and X. These were, (1) only IX nerve sectioned at the 1st gill arch (IX; n = 9), (2) first gill arch both IX and branch of X sectioned (G1; n = 9), (3) all four gill arches both IX and X sectioned (G4; n = 8), (4) a control group (n = 8) and (5) a sham-operated group that underwent surgery but no nerves were sectioned (n = 4). A cannula was inserted into the ventral aorta to monitor changes in heart rate and blood pressure. Ventilation rate and amplitude was also monitored with a cannula through the roof of the buccal cavity. The fish were exposed to a gradual hypoxia from 140 to 10 mmHg. Either the internal and external surfaces of the gill were treated with NaCN to determine the receptors orientation. Results showed that the receptors related to the heart rate control and ventilation amplitude were distributed over all gill arches on both the internal and external surfaces, and the branches of the both nerves IX and X were involved in these responses. This pattern differs from that observed for the ventilation rat. The receptors involved in the control of this response are located internally to the first gill arch. Tairão also showed blood pressure control chemorecptors on the first gill arch, involved in a very efficient barostatic control. Extrabranchial O2 chemoreceptors were not identified in trairão. This differs from the hypoxia tolerant fish, where extrabranchial chemoreceptors are important to start the cardio-respiratory responses the most fast and accurate possible |
id |
SCAR_c5cafa17882b5c41a66410f13dd6a9bd |
---|---|
oai_identifier_str |
oai:repositorio.ufscar.br:ufscar/1289 |
network_acronym_str |
SCAR |
network_name_str |
Repositório Institucional da UFSCAR |
repository_id_str |
4322 |
spelling |
Micheli, Mariana Andrade deRantin, Francisco Tadeuhttp://lattes.cnpq.br/3546242299713690http://lattes.cnpq.br/7871812883659034bdf4eec7-33e7-4f33-920d-934fe5cf28492016-06-02T19:22:48Z2008-03-042016-06-02T19:22:48Z2008-01-24MICHELI, Mariana Andrade de. Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental. 2008. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2008.https://repositorio.ufscar.br/handle/ufscar/1289The ability to sense and respond to oxygen availability in the environment is of extreme functional importance for fish. This is even more so in tropical freshwater habitats where environmental hypoxia can occur rapidly in space and time. Recent physiological studies on the cardio-respiratory response of tropical fishes have focused on hypoxia tolerant species. This aim of this study was to examine the neural control and function of the cardio-respiratory system in a fish that is adapted to living at high levels of oxygen. Trairão, Hoplias lacerdae was chosen because it is a member of the Erythrinidae family, in which most of species are hypoxia tolerant, including some where the O2 receptors has already been characterized. The study focused on investigation of the localization, distribution and orientation of chemoreceptors of O2 in trairão. Also how the cardio-respiratory parameters were modified by hypoxia. The fish were submitted to selective denervations of the branchial branches of the cranial nerves IX and X. These were, (1) only IX nerve sectioned at the 1st gill arch (IX; n = 9), (2) first gill arch both IX and branch of X sectioned (G1; n = 9), (3) all four gill arches both IX and X sectioned (G4; n = 8), (4) a control group (n = 8) and (5) a sham-operated group that underwent surgery but no nerves were sectioned (n = 4). A cannula was inserted into the ventral aorta to monitor changes in heart rate and blood pressure. Ventilation rate and amplitude was also monitored with a cannula through the roof of the buccal cavity. The fish were exposed to a gradual hypoxia from 140 to 10 mmHg. Either the internal and external surfaces of the gill were treated with NaCN to determine the receptors orientation. Results showed that the receptors related to the heart rate control and ventilation amplitude were distributed over all gill arches on both the internal and external surfaces, and the branches of the both nerves IX and X were involved in these responses. This pattern differs from that observed for the ventilation rat. The receptors involved in the control of this response are located internally to the first gill arch. Tairão also showed blood pressure control chemorecptors on the first gill arch, involved in a very efficient barostatic control. Extrabranchial O2 chemoreceptors were not identified in trairão. This differs from the hypoxia tolerant fish, where extrabranchial chemoreceptors are important to start the cardio-respiratory responses the most fast and accurate possibleA capacidade de sentir e responder às variações de oxigênio no ambiente é de extrema importância para peixes. Isto é mais acentuado em ambientes dulceaquícolas tropicais onde a hipóxia ambiental ocorre freqüentemente. Estudos recentes sobre respostas cardio-respiratórias em peixes tropicais têm enfocado espécies tolerantes à hipóxia. O foco do presente estudo foi examinar o controle neural e a função cardio-respiratória de um peixe adaptado a ambientes bem oxigenados. O trairão, Hoplias lacerdae foi escolhido por pertencer à família Erythrinidae, que apresenta a maioria das espécies tolerantes à hipóxia, incluindo algumas onde os receptores de O2 já foram caracterizados. O foco do estudo foi investigar a localização, distribuição e orientação dos quimiorreceptores de O2 em trairão, bem como os parâmetros cardio-respiratórios são modificados pela hipóxia. Os peixes foram submetidos à denervações seletivas dos ramos branquiais dos nervos cranianos IX e X. Os grupos foram: (1) apenas nervo IX seccionado do 1o arco branquial (IX; n = 9), (2) primeiro arco, ambos IX e ramo do X seccionados (G1; n = 9), (3) os quatro arcos, ambos IX e X seccionados (G4; n = 8), (4) grupo controle (n = 8) e (5) grupo sham-operated , que passou por cirurgia, mas não teve os nervos seccionados (n = 4). Uma cânula foi inserida na aorta ventral para monitorar freqüência cardíaca e pressão arterial. A freqüência e a amplitude ventilatórias foram monitoradas por uma cânula no palato da cavidade bucal. Os peixes foram expostos à hipóxia gradual de 140 a 10 mmHg. Ambas as superfícies, interna e externa, das brânquias receberam tratamento de NaCN para determinar a orientação dos receptores. Os resultados mostraram que receptores relacionados ao controle da freqüência cardíaca e amplitude ventilatória estão distribuídos por todos os arcos branquiais, orientados tanto interna quanto externamente, e ambos os nervos IX e X estão envolvidos nesta resposta. Este padrão difere do observado para a freqüência respiratória. Os receptores envolvidos no controle dessa resposta localizam-se internamente no 1o arco branquial. O trairão também possiu quimiorreceptores de O2 envolvidos no controle da pressão arterial apenas no primeiro arco branquial, participando de um controle barostático eficiente. Não foram identificados receptores de O2 extrabranquiais em trairão. Este padrão difere da maioria dos peixes tolerantes à hipóxia onde os estes receptores são importantes para iniciar rapidamente as respostas cardio-respiratórias à hipóxiaUniversidade Federal de Minas Geraisapplication/pdfporUniversidade Federal de São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarBRFisiologia comparadaQuimiorreceptoresErythrinidaeFunção cardio-respiratóriaCIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA DA RESPIRACAOCaracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambientalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-14fc5a788-1df7-480f-8123-b74f3f0306e1info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL1659.pdfapplication/pdf645648https://repositorio.ufscar.br/bitstream/ufscar/1289/1/1659.pdf6374964c1d7092a983850eef50d2bd02MD51THUMBNAIL1659.pdf.jpg1659.pdf.jpgIM Thumbnailimage/jpeg7901https://repositorio.ufscar.br/bitstream/ufscar/1289/2/1659.pdf.jpg939532e59a78973afecef5897da2f7f6MD52ufscar/12892023-09-18 18:31:52.573oai:repositorio.ufscar.br:ufscar/1289Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:52Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
title |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
spellingShingle |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental Micheli, Mariana Andrade de Fisiologia comparada Quimiorreceptores Erythrinidae Função cardio-respiratória CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA DA RESPIRACAO |
title_short |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
title_full |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
title_fullStr |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
title_full_unstemmed |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
title_sort |
Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental |
author |
Micheli, Mariana Andrade de |
author_facet |
Micheli, Mariana Andrade de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/7871812883659034 |
dc.contributor.author.fl_str_mv |
Micheli, Mariana Andrade de |
dc.contributor.advisor1.fl_str_mv |
Rantin, Francisco Tadeu |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3546242299713690 |
dc.contributor.authorID.fl_str_mv |
bdf4eec7-33e7-4f33-920d-934fe5cf2849 |
contributor_str_mv |
Rantin, Francisco Tadeu |
dc.subject.por.fl_str_mv |
Fisiologia comparada Quimiorreceptores Erythrinidae Função cardio-respiratória |
topic |
Fisiologia comparada Quimiorreceptores Erythrinidae Função cardio-respiratória CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA DA RESPIRACAO |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA DA RESPIRACAO |
description |
The ability to sense and respond to oxygen availability in the environment is of extreme functional importance for fish. This is even more so in tropical freshwater habitats where environmental hypoxia can occur rapidly in space and time. Recent physiological studies on the cardio-respiratory response of tropical fishes have focused on hypoxia tolerant species. This aim of this study was to examine the neural control and function of the cardio-respiratory system in a fish that is adapted to living at high levels of oxygen. Trairão, Hoplias lacerdae was chosen because it is a member of the Erythrinidae family, in which most of species are hypoxia tolerant, including some where the O2 receptors has already been characterized. The study focused on investigation of the localization, distribution and orientation of chemoreceptors of O2 in trairão. Also how the cardio-respiratory parameters were modified by hypoxia. The fish were submitted to selective denervations of the branchial branches of the cranial nerves IX and X. These were, (1) only IX nerve sectioned at the 1st gill arch (IX; n = 9), (2) first gill arch both IX and branch of X sectioned (G1; n = 9), (3) all four gill arches both IX and X sectioned (G4; n = 8), (4) a control group (n = 8) and (5) a sham-operated group that underwent surgery but no nerves were sectioned (n = 4). A cannula was inserted into the ventral aorta to monitor changes in heart rate and blood pressure. Ventilation rate and amplitude was also monitored with a cannula through the roof of the buccal cavity. The fish were exposed to a gradual hypoxia from 140 to 10 mmHg. Either the internal and external surfaces of the gill were treated with NaCN to determine the receptors orientation. Results showed that the receptors related to the heart rate control and ventilation amplitude were distributed over all gill arches on both the internal and external surfaces, and the branches of the both nerves IX and X were involved in these responses. This pattern differs from that observed for the ventilation rat. The receptors involved in the control of this response are located internally to the first gill arch. Tairão also showed blood pressure control chemorecptors on the first gill arch, involved in a very efficient barostatic control. Extrabranchial O2 chemoreceptors were not identified in trairão. This differs from the hypoxia tolerant fish, where extrabranchial chemoreceptors are important to start the cardio-respiratory responses the most fast and accurate possible |
publishDate |
2008 |
dc.date.available.fl_str_mv |
2008-03-04 2016-06-02T19:22:48Z |
dc.date.issued.fl_str_mv |
2008-01-24 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:22:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MICHELI, Mariana Andrade de. Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental. 2008. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2008. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/1289 |
identifier_str_mv |
MICHELI, Mariana Andrade de. Caracterização dos quimiorreceptores de O2 envolvidos no controle dos reflexos cardio-respiratórios de trairão, Hoplias lacerdae (Teleostei, Erythrinidae) em resposta à hipóxia ambiental. 2008. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2008. |
url |
https://repositorio.ufscar.br/handle/ufscar/1289 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.confidence.fl_str_mv |
-1 -1 |
dc.relation.authority.fl_str_mv |
4fc5a788-1df7-480f-8123-b74f3f0306e1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.publisher.program.fl_str_mv |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
dc.publisher.initials.fl_str_mv |
UFSCar |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFSCAR instname:Universidade Federal de São Carlos (UFSCAR) instacron:UFSCAR |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
UFSCAR |
institution |
UFSCAR |
reponame_str |
Repositório Institucional da UFSCAR |
collection |
Repositório Institucional da UFSCAR |
bitstream.url.fl_str_mv |
https://repositorio.ufscar.br/bitstream/ufscar/1289/1/1659.pdf https://repositorio.ufscar.br/bitstream/ufscar/1289/2/1659.pdf.jpg |
bitstream.checksum.fl_str_mv |
6374964c1d7092a983850eef50d2bd02 939532e59a78973afecef5897da2f7f6 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
|
_version_ |
1802136250819280896 |