Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores

Detalhes bibliográficos
Autor(a) principal: Buffo, Mariane Molina
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/7933
Resumo: The type and operational conditions of a bioreactor chosen during the production phase of a product of interest affect not only the morphology and growth of filamentous microorganisms but also the product itself. Still the most common process to produce bioproducts is submerged cultures in conventional stirred and aerated bioreactors, with impeller of type six flat-blade turbine, or Rushton turbine (RT), which promotes good mixing and suitable oxygen transfer, but its power consumption is high and it causes high shear rate to the broth creating a hostile environment to the microorganisms. Alternatively, an impeller of the type “Elephant Ear” (EE) is shown in the literature as a “low shear” impeller, more suitable for the cultivation of shear sensitive microorganisms. This impeller creates a mixed flow (axial and radial) of broth with down flow (EEDP) or up (EEUP) depending on its geometry. This study aimed to evaluate the best association of impellers for filamentous fungi cultures in a conventional bioreactor. Initially the volumetric coefficient of oxygen transfer (kLa) and the power consumption of seven different association of impellers were evaluated. The results obtained the factorial design methodology showed that the associations EEDP-EEUP, RT-EEDP, and EEDP-RT, showed the best results regarding the oxygen transfer and the power consumption, being up to 87% more efficient than the standard RT-RT association. Two of the better performing association and the traditional (RT-RT) were selected to be evaluated regarding the shearing, by using empirical equations and the size of the eddies, evaluated by the Kolmogorov microscale. The association that showed higher values on the Kolmogorov scale and least shearing was EEDPEEUP, with shearing up to 60% lower than the RT-RT association. In the last step the effects of shear on the morphology of the fungi Aspergillus niger was evaluated. Short-term cultures (4h) were cultivated so that the cellular growth would not harm the analysis. The results showed that on the culture that used the EEDP-EEUP association the morphological form of cell clumps predominated, while on the culture that used the RT-RT association the morphological form of branched hyphae predominated, suggesting that the RT-RT association causes more shearing and can cause irreversible damage to the fungal cells.
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spelling Buffo, Mariane MolinaBadino Júnior, Alberto Collihttp://lattes.cnpq.br/6244428434217018Farinas, Cristiane Sanchezhttp://lattes.cnpq.br/9933650905615452http://lattes.cnpq.br/4067015829295547b558d5a9-e4e2-4619-a964-f6e2de29b7cb2016-10-20T13:45:03Z2016-10-20T13:45:03Z2016-02-26BUFFO, Mariane Molina. Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores. 2016. Dissertação (Mestrado em Engenharia Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7933.https://repositorio.ufscar.br/handle/ufscar/7933The type and operational conditions of a bioreactor chosen during the production phase of a product of interest affect not only the morphology and growth of filamentous microorganisms but also the product itself. Still the most common process to produce bioproducts is submerged cultures in conventional stirred and aerated bioreactors, with impeller of type six flat-blade turbine, or Rushton turbine (RT), which promotes good mixing and suitable oxygen transfer, but its power consumption is high and it causes high shear rate to the broth creating a hostile environment to the microorganisms. Alternatively, an impeller of the type “Elephant Ear” (EE) is shown in the literature as a “low shear” impeller, more suitable for the cultivation of shear sensitive microorganisms. This impeller creates a mixed flow (axial and radial) of broth with down flow (EEDP) or up (EEUP) depending on its geometry. This study aimed to evaluate the best association of impellers for filamentous fungi cultures in a conventional bioreactor. Initially the volumetric coefficient of oxygen transfer (kLa) and the power consumption of seven different association of impellers were evaluated. The results obtained the factorial design methodology showed that the associations EEDP-EEUP, RT-EEDP, and EEDP-RT, showed the best results regarding the oxygen transfer and the power consumption, being up to 87% more efficient than the standard RT-RT association. Two of the better performing association and the traditional (RT-RT) were selected to be evaluated regarding the shearing, by using empirical equations and the size of the eddies, evaluated by the Kolmogorov microscale. The association that showed higher values on the Kolmogorov scale and least shearing was EEDPEEUP, with shearing up to 60% lower than the RT-RT association. In the last step the effects of shear on the morphology of the fungi Aspergillus niger was evaluated. Short-term cultures (4h) were cultivated so that the cellular growth would not harm the analysis. The results showed that on the culture that used the EEDP-EEUP association the morphological form of cell clumps predominated, while on the culture that used the RT-RT association the morphological form of branched hyphae predominated, suggesting that the RT-RT association causes more shearing and can cause irreversible damage to the fungal cells.O crescimento celular e a morfologia de microrganismos filamentosos, bem como o produto de interesse são afetados pelo modelo de biorreator e pelas condições de operação adotadas durante a etapa de produção. O processo mais adotado industrialmente para produção de bioprodutos ainda são os cultivos submersos em biorreatores convencionais tipo tanque agitado e aerado, sendo o impelidor tipo turbina de seis pás planas ou turbina de Rushton (RT) o mais utilizado por promover boa mistura e adequada transferência de oxigênio, porém seu consumo de potência é alto além de impor alto cisalhamento ao caldo gerando um ambiente hostil ao microrganismo. Alternativamente, o impelidor tipo “orelha de Elefante” ou “Elephant ear” (EE) é apresentado na literatura como um impelidor de “baixo cisalhamento” mais adequado para o cultivo dos microrganismos sensíveis ao cisalhamento. Esse impelidor promove um escoamento misto (axial e radial) do caldo com escoamento para baixo (EEDP) ou para cima (EEUP) dependendo da sua geometria. O presente trabalho teve como objetivo avaliar as melhores associações de impelidores para cultivos de fungos filamentosos em biorreator convencional. Primeiramente sete diferentes associações foram avaliadas em relação ao coeficiente volumétrico de transferência de oxigênio (kLa) e consumo de energia. Os resultados obtidos utilizando a metodologia de planejamento experimental fatorial mostraram que as configurações EEDP-EEUP, RT-EEDP e EEDP-RT foram as que apresentaram melhores resultados em relação à transferência de oxigênio e consumo de potência, com eficiência até 87% superior à associação padrão RT-RT. Foram então selecionadas duas das associações de melhor desempenho e a tradicional (RT-RT) para serem avaliadas em relação ao cisalhamento, através de equações empíricas e em relação ao tamanho dos turbilhões, avaliado pela microescala de Kolmogorov. A associação que apresentou maiores valores para microescala de Kolmogorov e menor cisalhamento foi a EEDP-EEUP, com cisalhamento até 60% inferior que a observada quando utilizada a associação RT-RT. Na última etapa verificou-se os efeitos do cisalhamento na morfologia do fungo Aspergillus niger. Foram realizados cultivos de curta duração (4 h) para evitar que o crescimento celular prejudicasse a análise. Os resultados mostraram que no cultivo utilizando o sistema EEDP-EEUP predominou a forma morfológica de aglomerados celulares (clumps), enquanto que no cultivo com impelidores Rushton (RT-RT) predominou a forma morfológica de hifas ramificadas, sugerindo um cisalhamento mais intenso provocado por este sistema de agitação, que pode acarretar danos irreversíveis às células fúngicas.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Engenharia Química - PPGEQUFSCarAssociações de impelidoresImpelidor orelha de elefanteTurbina RushtonTransferência de oxigênioConsumo de potênciaCisalhamentoAspergillus nigerImpellers associationsElephant ear impellerRushton turbineOxygen transferPower consumptionShearAspergillus nigerCIENCIAS EXATAS E DA TERRATransferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidoresinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline60060029d76c9b-aac3-4e11-bdb2-5988dc628937info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissMMB.pdfDissMMB.pdfapplication/pdf2553777https://repositorio.ufscar.br/bitstream/ufscar/7933/1/DissMMB.pdf21e951a2087a84913403141b819234c7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/7933/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissMMB.pdf.txtDissMMB.pdf.txtExtracted texttext/plain152377https://repositorio.ufscar.br/bitstream/ufscar/7933/3/DissMMB.pdf.txtbd0addc23325a36f1170c3486528f714MD53THUMBNAILDissMMB.pdf.jpgDissMMB.pdf.jpgIM Thumbnailimage/jpeg5995https://repositorio.ufscar.br/bitstream/ufscar/7933/4/DissMMB.pdf.jpg33824574a529e559c51bc112ec8cd227MD54ufscar/79332023-09-18 18:31:01.355oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:01Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
title Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
spellingShingle Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
Buffo, Mariane Molina
Associações de impelidores
Impelidor orelha de elefante
Turbina Rushton
Transferência de oxigênio
Consumo de potência
Cisalhamento
Aspergillus niger
Impellers associations
Elephant ear impeller
Rushton turbine
Oxygen transfer
Power consumption
Shear
Aspergillus niger
CIENCIAS EXATAS E DA TERRA
title_short Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
title_full Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
title_fullStr Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
title_full_unstemmed Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
title_sort Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores
author Buffo, Mariane Molina
author_facet Buffo, Mariane Molina
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/4067015829295547
dc.contributor.author.fl_str_mv Buffo, Mariane Molina
dc.contributor.advisor1.fl_str_mv Badino Júnior, Alberto Colli
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6244428434217018
dc.contributor.advisor-co1.fl_str_mv Farinas, Cristiane Sanchez
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9933650905615452
dc.contributor.authorID.fl_str_mv b558d5a9-e4e2-4619-a964-f6e2de29b7cb
contributor_str_mv Badino Júnior, Alberto Colli
Farinas, Cristiane Sanchez
dc.subject.por.fl_str_mv Associações de impelidores
Impelidor orelha de elefante
Turbina Rushton
Transferência de oxigênio
Consumo de potência
Cisalhamento
Aspergillus niger
topic Associações de impelidores
Impelidor orelha de elefante
Turbina Rushton
Transferência de oxigênio
Consumo de potência
Cisalhamento
Aspergillus niger
Impellers associations
Elephant ear impeller
Rushton turbine
Oxygen transfer
Power consumption
Shear
Aspergillus niger
CIENCIAS EXATAS E DA TERRA
dc.subject.eng.fl_str_mv Impellers associations
Elephant ear impeller
Rushton turbine
Oxygen transfer
Power consumption
Shear
Aspergillus niger
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA
description The type and operational conditions of a bioreactor chosen during the production phase of a product of interest affect not only the morphology and growth of filamentous microorganisms but also the product itself. Still the most common process to produce bioproducts is submerged cultures in conventional stirred and aerated bioreactors, with impeller of type six flat-blade turbine, or Rushton turbine (RT), which promotes good mixing and suitable oxygen transfer, but its power consumption is high and it causes high shear rate to the broth creating a hostile environment to the microorganisms. Alternatively, an impeller of the type “Elephant Ear” (EE) is shown in the literature as a “low shear” impeller, more suitable for the cultivation of shear sensitive microorganisms. This impeller creates a mixed flow (axial and radial) of broth with down flow (EEDP) or up (EEUP) depending on its geometry. This study aimed to evaluate the best association of impellers for filamentous fungi cultures in a conventional bioreactor. Initially the volumetric coefficient of oxygen transfer (kLa) and the power consumption of seven different association of impellers were evaluated. The results obtained the factorial design methodology showed that the associations EEDP-EEUP, RT-EEDP, and EEDP-RT, showed the best results regarding the oxygen transfer and the power consumption, being up to 87% more efficient than the standard RT-RT association. Two of the better performing association and the traditional (RT-RT) were selected to be evaluated regarding the shearing, by using empirical equations and the size of the eddies, evaluated by the Kolmogorov microscale. The association that showed higher values on the Kolmogorov scale and least shearing was EEDPEEUP, with shearing up to 60% lower than the RT-RT association. In the last step the effects of shear on the morphology of the fungi Aspergillus niger was evaluated. Short-term cultures (4h) were cultivated so that the cellular growth would not harm the analysis. The results showed that on the culture that used the EEDP-EEUP association the morphological form of cell clumps predominated, while on the culture that used the RT-RT association the morphological form of branched hyphae predominated, suggesting that the RT-RT association causes more shearing and can cause irreversible damage to the fungal cells.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-10-20T13:45:03Z
dc.date.available.fl_str_mv 2016-10-20T13:45:03Z
dc.date.issued.fl_str_mv 2016-02-26
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BUFFO, Mariane Molina. Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores. 2016. Dissertação (Mestrado em Engenharia Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7933.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/7933
identifier_str_mv BUFFO, Mariane Molina. Transferência de oxigênio e cisalhamento em biorreator convencional com diferentes combinações de impelidores. 2016. Dissertação (Mestrado em Engenharia Química) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7933.
url https://repositorio.ufscar.br/handle/ufscar/7933
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dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Engenharia Química - PPGEQ
dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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