Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/12181 |
Resumo: | In order to elucidate the role of skeletal muscle REST (RE1 Silencing Transcription Factor) protein on body composition and muscle functionality, we aimed at evaluating the effects of the specific deletion of REST from skeletal muscle (REST MKO) on mouse morphology, in vivo muscle strength and locomotor activity, and in vitro mechanical properties of isolated fast and slow skeletal muscles. Twenty-five mice were used in our study: 12 wild type (WT; 6 males and 6 females) and 13 REST MKO (8 males and 5 females). After 2 weeks of acclimation, the animals were housed in cages mounted with a running wheel (Day 0) so as to assess the level of voluntary physical activity. Subsequently (Day 4), the animals returned to standard cages and 3 days later (Day 7) grip strength and body composition were assessed. Euthanasia was carried out in the following two to four days (Days 9, 10 and 11; n = 2-4 mice/day) during which slow-twitch soleus (SOL) and fast-twitch extensor digitorum longus (EDL) muscles were collected to assess their mechanical properties during electrical-stimulation protocols. No statistical differences between REST MKO and WT mice were found in female mice. Based on this observation, only the data obtained in male mice were used. REST MKO had no significant effect on body weight, relative grip strength and voluntary physical activity; however, it increased fat (+9%) and decreased lean (-9%) masses significantly. Muscle relative weight was 31% higher in SOL and 19% smaller in the EDL of the REST MKO as compared to WT group (both p<0,05). Evaluation of isolated skeletal muscles mechanical properties during electrostimulation protocols indicated that REST MKO had no effect on maximal relative force in both the SOL and EDL. Regarding the muscle effects, REST MKO promoted greater force development and reduced fatigability in the EDL during the fatigue protocol. In contrast, in SOL muscles the deletion of REST led to a decrease in force production at low frequencies of stimulation and greater fatigue. We have shown that skeletal muscle REST protein seems to be important for the maintenance of lean mass and its loss leads to functional implications in different skeletal muscle tissues. |
id |
SCAR_cfde87b40c1a62fb65c8eae332cd2e2a |
---|---|
oai_identifier_str |
oai:repositorio.ufscar.br:ufscar/12181 |
network_acronym_str |
SCAR |
network_name_str |
Repositório Institucional da UFSCAR |
repository_id_str |
4322 |
spelling |
Canevazzi, Gustavo Henrique RigoPerez, Sérgio Eduardo de Andradehttp://lattes.cnpq.br/9408953831211309Bergeron, Raynaldhttp://lattes.cnpq.br/22407912389254551178dbe0-b32b-42fa-b721-dd670addd8a82020-01-29T16:27:12Z2020-01-29T16:27:12Z2018-10-26CANEVAZZI, Gustavo Henrique Rigo. Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/12181.https://repositorio.ufscar.br/handle/ufscar/12181In order to elucidate the role of skeletal muscle REST (RE1 Silencing Transcription Factor) protein on body composition and muscle functionality, we aimed at evaluating the effects of the specific deletion of REST from skeletal muscle (REST MKO) on mouse morphology, in vivo muscle strength and locomotor activity, and in vitro mechanical properties of isolated fast and slow skeletal muscles. Twenty-five mice were used in our study: 12 wild type (WT; 6 males and 6 females) and 13 REST MKO (8 males and 5 females). After 2 weeks of acclimation, the animals were housed in cages mounted with a running wheel (Day 0) so as to assess the level of voluntary physical activity. Subsequently (Day 4), the animals returned to standard cages and 3 days later (Day 7) grip strength and body composition were assessed. Euthanasia was carried out in the following two to four days (Days 9, 10 and 11; n = 2-4 mice/day) during which slow-twitch soleus (SOL) and fast-twitch extensor digitorum longus (EDL) muscles were collected to assess their mechanical properties during electrical-stimulation protocols. No statistical differences between REST MKO and WT mice were found in female mice. Based on this observation, only the data obtained in male mice were used. REST MKO had no significant effect on body weight, relative grip strength and voluntary physical activity; however, it increased fat (+9%) and decreased lean (-9%) masses significantly. Muscle relative weight was 31% higher in SOL and 19% smaller in the EDL of the REST MKO as compared to WT group (both p<0,05). Evaluation of isolated skeletal muscles mechanical properties during electrostimulation protocols indicated that REST MKO had no effect on maximal relative force in both the SOL and EDL. Regarding the muscle effects, REST MKO promoted greater force development and reduced fatigability in the EDL during the fatigue protocol. In contrast, in SOL muscles the deletion of REST led to a decrease in force production at low frequencies of stimulation and greater fatigue. We have shown that skeletal muscle REST protein seems to be important for the maintenance of lean mass and its loss leads to functional implications in different skeletal muscle tissues.Para elucidar o papel da proteína REST (RE1 Silencing Transcription Factor) do músculo esquelético sobre a composição corporal e funcionalidade muscular de camundongos adultos, objetivamos avaliar os efeitos da deleção específica de REST do músculo esquelético (REST MKO) sobre a morfologia de camundongos, força muscular in vivo e atividade locomotora e propriedades mecânicas in vitro de músculos esqueléticos de contração rápida e lenta isolados. Vinte e cinco camundongos foram utilizados em nosso estudo: 12 do tipo selvagem (WT; 6 machos e 6 fêmeas) e 13 REST MKO (8 machos e 5 fêmeas). Após 2 semanas de aclimatação, os animais foram alojados em gaiolas montadas com uma roda de corrida (Dia 0) para avaliar o nível de atividade física voluntária. Subsequentemente (Dia 4), os animais retornaram às gaiolas padrão e 3 dias depois (Dia 7) foram avaliadas a força de preensão e a composição corporal. A eutanásia foi realizada de dois a quatro dias depois da última intervenção (dias 9, 10 e 11; n = 2-4 camundongos/dia) durante os quais foram coletados os músculos de contração lenta soleus (SOL) e o de contração rápida extensor longo dos dedos (EDL) para avaliar suas propriedades mecânicas durante protocolos de estimulação elétrica. Não foram encontradas diferenças estatísticas entre camundongos REST MKO e WT em camundongos fêmeas. Com base nessa observação, apenas os dados obtidos em camundongos machos foram utilizados. REST MKO não apresentou efeito significativo no peso corporal, força de preensão relativa e atividade física voluntária; no entanto, REST MKO aumentou significativamente a massa gorda (+ 9%) e diminuiu a massa magra (-9%). O peso relativo do músculo foi 31% maior no SOL e 19% menor no EDL do REST MKO em relação ao grupo WT (ambos p <0,05). A avaliação das propriedades mecânicas dos músculos esqueléticos isolados durante os protocolos de eletroestimulação indicou que REST MKO não apresentou efeito sobre a força relativa máxima no SOL e no EDL. Em relação aos efeitos musculares, o REST MKO promoveu maior desenvolvimento de força e reduziu fadiga no EDL durante o protocolo de fadiga muscular. Em contraste, nos músculos SOL a deleção de REST levou a uma diminuição na produção de força nas baixas frequências de estimulação e maior fadiga. Nós mostramos que o fator de transcrição REST do músculo esquelético parece ser importante para a manutenção da massa magra e sua perda leva a implicações funcionais em diferentes tecidos musculares esqueléticos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: código de financiamento - 001engUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessRestSkeletal muscleForce-frequencyFatigueMúsculo esqueléticoForça-frequênciaFadigaCIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6Effects of skeletal muscle REST deletion on body composition and muscle function in C57BL/6 mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis6006004a93daf7-ae1b-430e-a168-f54fa5dcbc79reponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALCarta comprovante versão final de tese_Gustavo Canevazzi.pdfCarta comprovante versão final de tese_Gustavo Canevazzi.pdfCarta comprovante versão final de teseapplication/pdf136292https://repositorio.ufscar.br/bitstream/ufscar/12181/2/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdfa379c12331b4bacb69d7de6f146705a0MD52Final_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdfFinal_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdfVersão final de tese - Gustavo H. R. Canevazziapplication/pdf1463623https://repositorio.ufscar.br/bitstream/ufscar/12181/7/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdfcd788c184ee0471b2bddcabfbb067555MD57CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/12181/8/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD58TEXTCarta comprovante versão final de tese_Gustavo Canevazzi.pdf.txtCarta comprovante versão final de tese_Gustavo Canevazzi.pdf.txtExtracted texttext/plain1598https://repositorio.ufscar.br/bitstream/ufscar/12181/9/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdf.txt3932fae8d00ce344f0648dbf22ccd6a1MD59Final_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdf.txtFinal_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdf.txtExtracted texttext/plain103910https://repositorio.ufscar.br/bitstream/ufscar/12181/11/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdf.txt2b192be6cf410d2c45d1697a164738cbMD511THUMBNAILCarta comprovante versão final de tese_Gustavo Canevazzi.pdf.jpgCarta comprovante versão final de tese_Gustavo Canevazzi.pdf.jpgIM Thumbnailimage/jpeg15556https://repositorio.ufscar.br/bitstream/ufscar/12181/10/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdf.jpgf2c8b4d0b0f2ce7f57c3d0e51fdbfb87MD510Final_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdf.jpgFinal_Thesis_Gustavo Canevazzi_Effects of satellite cell REST deletion on body composition and muscle function in mice.pdf.jpgIM Thumbnailimage/jpeg9498https://repositorio.ufscar.br/bitstream/ufscar/12181/12/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdf.jpgc87b1420402649eba665e1aba7afc86aMD512ufscar/121812023-09-18 18:31:49.86oai:repositorio.ufscar.br:ufscar/12181Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:49Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
dc.title.alternative.eng.fl_str_mv |
Effects of skeletal muscle REST deletion on body composition and muscle function in C57BL/6 mice. |
title |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
spellingShingle |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 Canevazzi, Gustavo Henrique Rigo Rest Skeletal muscle Force-frequency Fatigue Músculo esquelético Força-frequência Fadiga CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
title_full |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
title_fullStr |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
title_full_unstemmed |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
title_sort |
Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6 |
author |
Canevazzi, Gustavo Henrique Rigo |
author_facet |
Canevazzi, Gustavo Henrique Rigo |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/2240791238925455 |
dc.contributor.author.fl_str_mv |
Canevazzi, Gustavo Henrique Rigo |
dc.contributor.advisor1.fl_str_mv |
Perez, Sérgio Eduardo de Andrade |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9408953831211309 |
dc.contributor.advisor-co1.fl_str_mv |
Bergeron, Raynald |
dc.contributor.authorID.fl_str_mv |
1178dbe0-b32b-42fa-b721-dd670addd8a8 |
contributor_str_mv |
Perez, Sérgio Eduardo de Andrade Bergeron, Raynald |
dc.subject.eng.fl_str_mv |
Rest Skeletal muscle Force-frequency Fatigue |
topic |
Rest Skeletal muscle Force-frequency Fatigue Músculo esquelético Força-frequência Fadiga CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.por.fl_str_mv |
Músculo esquelético Força-frequência Fadiga |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
In order to elucidate the role of skeletal muscle REST (RE1 Silencing Transcription Factor) protein on body composition and muscle functionality, we aimed at evaluating the effects of the specific deletion of REST from skeletal muscle (REST MKO) on mouse morphology, in vivo muscle strength and locomotor activity, and in vitro mechanical properties of isolated fast and slow skeletal muscles. Twenty-five mice were used in our study: 12 wild type (WT; 6 males and 6 females) and 13 REST MKO (8 males and 5 females). After 2 weeks of acclimation, the animals were housed in cages mounted with a running wheel (Day 0) so as to assess the level of voluntary physical activity. Subsequently (Day 4), the animals returned to standard cages and 3 days later (Day 7) grip strength and body composition were assessed. Euthanasia was carried out in the following two to four days (Days 9, 10 and 11; n = 2-4 mice/day) during which slow-twitch soleus (SOL) and fast-twitch extensor digitorum longus (EDL) muscles were collected to assess their mechanical properties during electrical-stimulation protocols. No statistical differences between REST MKO and WT mice were found in female mice. Based on this observation, only the data obtained in male mice were used. REST MKO had no significant effect on body weight, relative grip strength and voluntary physical activity; however, it increased fat (+9%) and decreased lean (-9%) masses significantly. Muscle relative weight was 31% higher in SOL and 19% smaller in the EDL of the REST MKO as compared to WT group (both p<0,05). Evaluation of isolated skeletal muscles mechanical properties during electrostimulation protocols indicated that REST MKO had no effect on maximal relative force in both the SOL and EDL. Regarding the muscle effects, REST MKO promoted greater force development and reduced fatigability in the EDL during the fatigue protocol. In contrast, in SOL muscles the deletion of REST led to a decrease in force production at low frequencies of stimulation and greater fatigue. We have shown that skeletal muscle REST protein seems to be important for the maintenance of lean mass and its loss leads to functional implications in different skeletal muscle tissues. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-10-26 |
dc.date.accessioned.fl_str_mv |
2020-01-29T16:27:12Z |
dc.date.available.fl_str_mv |
2020-01-29T16:27:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CANEVAZZI, Gustavo Henrique Rigo. Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/12181. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/12181 |
identifier_str_mv |
CANEVAZZI, Gustavo Henrique Rigo. Efeitos da deleção de REST do músculo esquelético sobre a composição corporal e função muscular de camundongos c57bl/6. 2018. Tese (Doutorado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2018. Disponível em: https://repositorio.ufscar.br/handle/ufscar/12181. |
url |
https://repositorio.ufscar.br/handle/ufscar/12181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.confidence.fl_str_mv |
600 600 |
dc.relation.authority.fl_str_mv |
4a93daf7-ae1b-430e-a168-f54fa5dcbc79 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
dc.publisher.initials.fl_str_mv |
UFSCar |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFSCAR instname:Universidade Federal de São Carlos (UFSCAR) instacron:UFSCAR |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
UFSCAR |
institution |
UFSCAR |
reponame_str |
Repositório Institucional da UFSCAR |
collection |
Repositório Institucional da UFSCAR |
bitstream.url.fl_str_mv |
https://repositorio.ufscar.br/bitstream/ufscar/12181/2/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdf https://repositorio.ufscar.br/bitstream/ufscar/12181/7/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdf https://repositorio.ufscar.br/bitstream/ufscar/12181/8/license_rdf https://repositorio.ufscar.br/bitstream/ufscar/12181/9/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/12181/11/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/12181/10/Carta%20comprovante%20vers%c3%a3o%20final%20de%20tese_Gustavo%20Canevazzi.pdf.jpg https://repositorio.ufscar.br/bitstream/ufscar/12181/12/Final_Thesis_Gustavo%20Canevazzi_Effects%20of%20satellite%20cell%20REST%20deletion%20on%20body%20composition%20and%20muscle%20function%20in%20mice.pdf.jpg |
bitstream.checksum.fl_str_mv |
a379c12331b4bacb69d7de6f146705a0 cd788c184ee0471b2bddcabfbb067555 e39d27027a6cc9cb039ad269a5db8e34 3932fae8d00ce344f0648dbf22ccd6a1 2b192be6cf410d2c45d1697a164738cb f2c8b4d0b0f2ce7f57c3d0e51fdbfb87 c87b1420402649eba665e1aba7afc86a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
|
_version_ |
1802136351229870080 |