Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/9453 |
Resumo: | The sugarcane is a major global economic importance culture, especially for Brazil. The country is the largest producer of culture and its by-products, sugar and alcohol. The sugarcane has received attention for the generation of electricity. Since the importance of the sugar-energy sector, there is a need to increase agricultural production. This culture is exposed to various diseases caused by pathogens that impair the production of culture. Coal disease is one of them, caused by the fungus S. Scitamineum, making it less susceptible productive varieties and can cause loss of up to 100% of the plantation. More productive varieties with resistance to more diseases, obtained in less time are focused on breeding programs. The study aims to find microsatellite markers (SSR) associated with resistance of sugarcane to the fungus S. scitamineum. It used an F1 population (SP81-3250 RB925345 x) composed of 238 genotypes. Artificial inoculation of sugarcane stalks of the population through telia injection was made. The billets were kept in a greenhouse, and 25 were carried out between January and September 2014 for the first experiment and August 2015 to February 2016 for the experiment II, to identify the incidence of the disease. Molecular analysis and construction of linkage map were made by genotyping with SSR markers, which enabled the construction of linkage map with 128 brands linked, generating 49 linkage groups with a total length of 1297.68 cm. For data on the incidence of coal was used to calculate the area under the disease progress curve (AACPD), normalized to BLUPS values. The analysis allowed us to observe a population with more tolerant genotypes in experiment I and little disease in experiment II. In addition, it was noted that for the first experiment, the 10th to the 25th evaluation, the results were highly correlated, it was not repeated for the second trial. Experiment II showed low incidence in all genotypes of the population and patterns in the study. This incidence difference between the two experiments probably occurred by the difference in temperature conditions to which they were exposed, and the first experiment was set up in the summer with a starting temperature higher and the second trial began with mild temperatures and were increasing with time, the same occurs for the incidence of the disease. The association between phenotype and genotype was performed by analysis of variance, converted to LOD score. It was possible to verify an EST-SSR marker, SCB 370, which was significant (LOD> 3), explaining in 5% of the phenotypic variation for the experiment I. |
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Silveira, Bianca RochaCarneiro, Monalisa Sampaiohttp://lattes.cnpq.br/2696490871291334http://lattes.cnpq.br/6171235025228767f63ff6a0-a2a6-4472-8984-fbf60c2ede752018-02-20T13:30:48Z2018-02-20T13:30:48Z2016-11-24SILVEIRA, Bianca Rocha. Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar. 2016. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9453.https://repositorio.ufscar.br/handle/ufscar/9453The sugarcane is a major global economic importance culture, especially for Brazil. The country is the largest producer of culture and its by-products, sugar and alcohol. The sugarcane has received attention for the generation of electricity. Since the importance of the sugar-energy sector, there is a need to increase agricultural production. This culture is exposed to various diseases caused by pathogens that impair the production of culture. Coal disease is one of them, caused by the fungus S. Scitamineum, making it less susceptible productive varieties and can cause loss of up to 100% of the plantation. More productive varieties with resistance to more diseases, obtained in less time are focused on breeding programs. The study aims to find microsatellite markers (SSR) associated with resistance of sugarcane to the fungus S. scitamineum. It used an F1 population (SP81-3250 RB925345 x) composed of 238 genotypes. Artificial inoculation of sugarcane stalks of the population through telia injection was made. The billets were kept in a greenhouse, and 25 were carried out between January and September 2014 for the first experiment and August 2015 to February 2016 for the experiment II, to identify the incidence of the disease. Molecular analysis and construction of linkage map were made by genotyping with SSR markers, which enabled the construction of linkage map with 128 brands linked, generating 49 linkage groups with a total length of 1297.68 cm. For data on the incidence of coal was used to calculate the area under the disease progress curve (AACPD), normalized to BLUPS values. The analysis allowed us to observe a population with more tolerant genotypes in experiment I and little disease in experiment II. In addition, it was noted that for the first experiment, the 10th to the 25th evaluation, the results were highly correlated, it was not repeated for the second trial. Experiment II showed low incidence in all genotypes of the population and patterns in the study. This incidence difference between the two experiments probably occurred by the difference in temperature conditions to which they were exposed, and the first experiment was set up in the summer with a starting temperature higher and the second trial began with mild temperatures and were increasing with time, the same occurs for the incidence of the disease. The association between phenotype and genotype was performed by analysis of variance, converted to LOD score. It was possible to verify an EST-SSR marker, SCB 370, which was significant (LOD> 3), explaining in 5% of the phenotypic variation for the experiment I.A cana-de-açúcar é uma cultura de grande importância econômica mundial, em especial para o Brasil. O país é o maior produtor da cultura e dos seus subprodutos, açúcar e álcool. A cana-de-açúcar vem recebendo atenção para a geração de eletricidade. Visto a importância do setor sucroenergético, há a necessidade de aumentar sua produção agrícola. Esta cultura está exposta a várias doenças, causadas por fitopatógenos, que prejudicam a produção da cultura. A doença do carvão é uma delas, causada pelo fungo Sporisorium scitamineum, tornando variedades suscetíveis menos produtivas e podem causar perda de até 100% da plantação. Variedades mais produtivas, com resistência a maior número de doenças, obtidas em menor tempo são enfoque em programas de melhoramento. O trabalho tem por objetivo encontrar marcadores moleculares microssatélites (SSR) associados à resistência da cana-de-açúcar ao fungo S. scitamineum. Foi utilizada uma população F1 (SP81-3250 x RB925345), composta por 238 genótipos. Foi feita a inoculação artificial de toletes de cana-de-açúcar na população por meio de injeção de teliósporos. Os toletes foram mantidos em casa de vegetação, sendo que 25 avaliações foram realizadas entre janeiro e setembro de 2014 para o experimento I e agosto de 2015 a fevereiro de 2016 para o experimento II, visando identificar a incidência da doença. Foram feitas análises moleculares e construção do mapa de ligação, pela genotipagem com marcadores SSR, o qual possibilitou a construção de mapa de ligação com 128 marcas ligadas, gerando 49 grupos de ligação com comprimento total de 1297,68 cM. Para os dados referentes à incidência de carvão foi utilizado o cálculo da área abaixo da curva de progresso da doença (AACPD), normalizados para valores de BLUPS. A análise permitiu observar uma população com maior número de genótipos tolerantes no experimento I e pouca doença no experimento II. Além disso, foi notado que para o experimento I, da 10ª à 25ª avaliação, os resultados tinham alta correlação, o mesmo não se repetiu para o experimento II. O experimento II apresentou baixa incidência em todos os genótipos da população e nos padrões em estudo. Esta diferença de incidência entre os dois experimentos provavelmente ocorreu pela diferença de condições de temperatura a qual eles foram expostos, sendo que o experimento I foi montado no verão com um início de temperatura mais alta e o experimento II começou com temperaturas amenas e que foram aumentando com o tempo, o mesmo ocorre para a incidência da doença. A associação entre fenótipo e genótipo foi realizada através da análise de variância, convertido para LOD Score. Foi possível verificar um marcador ESTSSR, SCB 370, que foi significativo (LOD > 3), explicando em 5% da variação fenotípica para o experimento I.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)porUniversidade Federal de São CarlosCâmpus ArarasPrograma de Pós-Graduação em Produção Vegetal e Bioprocessos Associados - PPGPVBA-ArUFSCarCana-de-açúcarSetor sucroenergéticoDoença do carvãoMarcadores moleculares microssatélitesSporisorium scitamineumCIENCIAS AGRARIASMapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcarQTL mapping for resistance to Sporisorium scitamineum in population bi-parental of sugarcaneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline6006008b00c020-1bcf-4189-9442-22f66f7fb97ainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissBRS.pdfDissBRS.pdfapplication/pdf3533795https://repositorio.ufscar.br/bitstream/ufscar/9453/1/DissBRS.pdf20466420310c818bf98e4bceec2120faMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/9453/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissBRS.pdf.txtDissBRS.pdf.txtExtracted texttext/plain141770https://repositorio.ufscar.br/bitstream/ufscar/9453/3/DissBRS.pdf.txtd4c2cb9e50964586a78cd2d6e5f96c80MD53THUMBNAILDissBRS.pdf.jpgDissBRS.pdf.jpgIM Thumbnailimage/jpeg6982https://repositorio.ufscar.br/bitstream/ufscar/9453/4/DissBRS.pdf.jpgf0518af32b124eeb42e8268ffe6c2417MD54ufscar/94532023-09-18 18:31:13.02oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:13Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
dc.title.alternative.eng.fl_str_mv |
QTL mapping for resistance to Sporisorium scitamineum in population bi-parental of sugarcane |
title |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
spellingShingle |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar Silveira, Bianca Rocha Cana-de-açúcar Setor sucroenergético Doença do carvão Marcadores moleculares microssatélites Sporisorium scitamineum CIENCIAS AGRARIAS |
title_short |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
title_full |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
title_fullStr |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
title_full_unstemmed |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
title_sort |
Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar |
author |
Silveira, Bianca Rocha |
author_facet |
Silveira, Bianca Rocha |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/6171235025228767 |
dc.contributor.author.fl_str_mv |
Silveira, Bianca Rocha |
dc.contributor.advisor1.fl_str_mv |
Carneiro, Monalisa Sampaio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2696490871291334 |
dc.contributor.authorID.fl_str_mv |
f63ff6a0-a2a6-4472-8984-fbf60c2ede75 |
contributor_str_mv |
Carneiro, Monalisa Sampaio |
dc.subject.por.fl_str_mv |
Cana-de-açúcar Setor sucroenergético Doença do carvão Marcadores moleculares microssatélites |
topic |
Cana-de-açúcar Setor sucroenergético Doença do carvão Marcadores moleculares microssatélites Sporisorium scitamineum CIENCIAS AGRARIAS |
dc.subject.lat.fl_str_mv |
Sporisorium scitamineum |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
The sugarcane is a major global economic importance culture, especially for Brazil. The country is the largest producer of culture and its by-products, sugar and alcohol. The sugarcane has received attention for the generation of electricity. Since the importance of the sugar-energy sector, there is a need to increase agricultural production. This culture is exposed to various diseases caused by pathogens that impair the production of culture. Coal disease is one of them, caused by the fungus S. Scitamineum, making it less susceptible productive varieties and can cause loss of up to 100% of the plantation. More productive varieties with resistance to more diseases, obtained in less time are focused on breeding programs. The study aims to find microsatellite markers (SSR) associated with resistance of sugarcane to the fungus S. scitamineum. It used an F1 population (SP81-3250 RB925345 x) composed of 238 genotypes. Artificial inoculation of sugarcane stalks of the population through telia injection was made. The billets were kept in a greenhouse, and 25 were carried out between January and September 2014 for the first experiment and August 2015 to February 2016 for the experiment II, to identify the incidence of the disease. Molecular analysis and construction of linkage map were made by genotyping with SSR markers, which enabled the construction of linkage map with 128 brands linked, generating 49 linkage groups with a total length of 1297.68 cm. For data on the incidence of coal was used to calculate the area under the disease progress curve (AACPD), normalized to BLUPS values. The analysis allowed us to observe a population with more tolerant genotypes in experiment I and little disease in experiment II. In addition, it was noted that for the first experiment, the 10th to the 25th evaluation, the results were highly correlated, it was not repeated for the second trial. Experiment II showed low incidence in all genotypes of the population and patterns in the study. This incidence difference between the two experiments probably occurred by the difference in temperature conditions to which they were exposed, and the first experiment was set up in the summer with a starting temperature higher and the second trial began with mild temperatures and were increasing with time, the same occurs for the incidence of the disease. The association between phenotype and genotype was performed by analysis of variance, converted to LOD score. It was possible to verify an EST-SSR marker, SCB 370, which was significant (LOD> 3), explaining in 5% of the phenotypic variation for the experiment I. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-11-24 |
dc.date.accessioned.fl_str_mv |
2018-02-20T13:30:48Z |
dc.date.available.fl_str_mv |
2018-02-20T13:30:48Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVEIRA, Bianca Rocha. Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar. 2016. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9453. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/9453 |
identifier_str_mv |
SILVEIRA, Bianca Rocha. Mapeamento de QTL para resistência a Sporisorium scitamineum em população bi-parental de cana-de-açúcar. 2016. Dissertação (Mestrado em Produção Vegetal e Bioprocessos Associados) – Universidade Federal de São Carlos, Araras, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/9453. |
url |
https://repositorio.ufscar.br/handle/ufscar/9453 |
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openAccess |
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Universidade Federal de São Carlos Câmpus Araras |
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Programa de Pós-Graduação em Produção Vegetal e Bioprocessos Associados - PPGPVBA-Ar |
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UFSCar |
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Universidade Federal de São Carlos Câmpus Araras |
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