Comparação do perfil de metabólitos secundários de cultivares de soja
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/15059 |
Resumo: | Soybean (Glycine max) is one of the most important crops in the world socioeconomic scenario, especially in Brazil. However, this crop is constantly attacked by different pests. Plant resistance against herbivores occurs partially through the production of secondary metabolites such as flavonoids. Wild soybeans and cultivated varieties exhibit different levels of resistance to agricultural pests, which may be a consequence of the variability in their secondary metabolite profiles. Thus, identifying these substances among different genotypes and understanding their role in the insect-plant interaction mechanisms is important for a more comprehensive and reliable characterization of soybean resistance. This work proposes to compare the profile of secondary metabolites between 4 cultivated soybean varieties resistant (PI 229358 and IAC 100) and susceptible (BRS 257 and IAC PL-1) to pests and a wild soybean variety (PI 407270). The chemical profile of young and old leaves in the V3 development stage was analyzed. Plants were grown in a greenhouse and different samples were extracted and analyzed by LC-MS. The analysis of the flavonoid profile in the 5 varieties allowed the identification of 18 isoflavone conjugates (daidzein, genistein and glycitein), some of which were present in greater abundance in old leaves. 39 flavonols were also detected, including kaempferol and quercetin conjugates; accumulated mostly in young leaves. Furthermore, it was shown that wild soybeans have higher levels of flavonoids in their younger leaves, in contrast to cultivated varieties. This information will allow us to understand the dynamics of flavonoids in the insect-plant interaction mechanisms and contribute to the creation of resistant varieties and new targets for the management of agricultural pests, contributing to the sustainable development of agriculture. |
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Arroyo, Victoria Patricia PenaWouters, Felipe Christoffhttp://lattes.cnpq.br/4392349445398645http://lattes.cnpq.br/9409311086029770c87def44-798b-48d0-a83a-7a1c3e81447e2021-10-28T10:35:01Z2021-10-28T10:35:01Z2021-07-29ARROYO, Victoria Patricia Pena. Comparação do perfil de metabólitos secundários de cultivares de soja. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15059.https://repositorio.ufscar.br/handle/ufscar/15059Soybean (Glycine max) is one of the most important crops in the world socioeconomic scenario, especially in Brazil. However, this crop is constantly attacked by different pests. Plant resistance against herbivores occurs partially through the production of secondary metabolites such as flavonoids. Wild soybeans and cultivated varieties exhibit different levels of resistance to agricultural pests, which may be a consequence of the variability in their secondary metabolite profiles. Thus, identifying these substances among different genotypes and understanding their role in the insect-plant interaction mechanisms is important for a more comprehensive and reliable characterization of soybean resistance. This work proposes to compare the profile of secondary metabolites between 4 cultivated soybean varieties resistant (PI 229358 and IAC 100) and susceptible (BRS 257 and IAC PL-1) to pests and a wild soybean variety (PI 407270). The chemical profile of young and old leaves in the V3 development stage was analyzed. Plants were grown in a greenhouse and different samples were extracted and analyzed by LC-MS. The analysis of the flavonoid profile in the 5 varieties allowed the identification of 18 isoflavone conjugates (daidzein, genistein and glycitein), some of which were present in greater abundance in old leaves. 39 flavonols were also detected, including kaempferol and quercetin conjugates; accumulated mostly in young leaves. Furthermore, it was shown that wild soybeans have higher levels of flavonoids in their younger leaves, in contrast to cultivated varieties. This information will allow us to understand the dynamics of flavonoids in the insect-plant interaction mechanisms and contribute to the creation of resistant varieties and new targets for the management of agricultural pests, contributing to the sustainable development of agriculture.A soja, (Glycine max), é uma das culturas mais importantes no cenário socioeconômico mundial, em especial no Brasil. No entanto, esta cultura é constantemente atacada por distintas pragas. A resistência das plantas contra herbívoros ocorre em parte pela produção de metabólitos secundários, como os flavonoides. Tanto a soja selvagem como as variedades cultivadas exibem diferentes níveis de resistência a pragas agrícolas, o que pode ser consequência da variabilidade nos seus perfis de metabólitos secundários. Assim, identificar estas substâncias entre distintos genótipos e entender o papel que têm nos mecanismos de interação inseto-planta é importante para uma caracterização mais abrangente e confiável da resistência da soja. Deste modo, este trabalho propõe-se comparar o perfil de metabólitos secundários entre 4 variedades de soja cultivada resistentes (PI 229358 e IAC 100) e susceptíveis (BRS 257 e IAC PL-1) a pragas e uma variedade de soja selvagem (PI 407270). Foi analisado o perfil químico em folhas jovens e velhas no estágio de desenvolvimento V3. As plantas foram cultivadas em casa de vegetação e as diferentes amostras foram extraídas e analisadas por LC-MS. A análise do perfil de flavonoides nas 5 variedades permitiu a identificação de 18 conjugados de isoflavonas (daidzeína, genisteína e gliciteína), alguns dos quais estavam presentes em maior abundância nas folhas velhas. Também foram detectados 39 flavonóis, incluindo conjugados de kaempferol e quercetina; acumulados em sua maioria nas folhas jovens. Ademais, foi evidenciado que a soja selvagem apresenta níveis mais altos de flavonoides nas suas folhas mais jovens, em contraste com as variedades cultivadas. Estas informações nos permitirão entender a dinâmica dos flavonóides nos mecanismos de interação inseto-planta e deste modo, contribuir na criação de variedades resistentes e novos alvos para o manejo de pragas agrícolas, contribuindo para o desenvolvimento sustentável da agricultura.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: Código de Financiamento 001porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Química - PPGQUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessSojaDomesticaçãoPerfil químicoFlavonoidesSoybeanDomesticationChemical profileFlavonoidsCIENCIAS EXATAS E DA TERRA::QUIMICACIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::EVOLUCAO, SISTEMATICA E ECOLOGIA QUIMICACIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAISComparação do perfil de metabólitos secundários de cultivares de sojaComparison of the secondary metabolites profile of soybean cultivarsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6006004dc1e857-50fd-410f-8d79-bd44db1224adreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDISSERTAÇÃO-Victoria-2021 V5-pdf.pdfDISSERTAÇÃO-Victoria-2021 V5-pdf.pdfDissertação de Mestrado-Victoria Penaapplication/pdf4257295https://repositorio.ufscar.br/bitstream/ufscar/15059/1/DISSERTA%c3%87%c3%83O-Victoria-2021%20V5-pdf.pdfd4be44c7208ac70ab0c07e366ff2c2fbMD51Carta comprovante homologacao- Victoria Peña.pdfCarta comprovante homologacao- Victoria Peña.pdfCarta Comprovante de versão finalapplication/pdf158841https://repositorio.ufscar.br/bitstream/ufscar/15059/2/Carta%20comprovante%20homologacao-%20Victoria%20Pe%c3%b1a.pdf2e565ff44a911a29cca1ffdc3273d98eMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15059/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDISSERTAÇÃO-Victoria-2021 V5-pdf.pdf.txtDISSERTAÇÃO-Victoria-2021 V5-pdf.pdf.txtExtracted texttext/plain166005https://repositorio.ufscar.br/bitstream/ufscar/15059/4/DISSERTA%c3%87%c3%83O-Victoria-2021%20V5-pdf.pdf.txt59f722521776c6f321f65f91d2c45a2fMD54Carta comprovante homologacao- Victoria Peña.pdf.txtCarta comprovante homologacao- Victoria Peña.pdf.txtExtracted texttext/plain1431https://repositorio.ufscar.br/bitstream/ufscar/15059/6/Carta%20comprovante%20homologacao-%20Victoria%20Pe%c3%b1a.pdf.txt83e1ccba44743ef44561ec70088bedc7MD56THUMBNAILDISSERTAÇÃO-Victoria-2021 V5-pdf.pdf.jpgDISSERTAÇÃO-Victoria-2021 V5-pdf.pdf.jpgIM Thumbnailimage/jpeg8484https://repositorio.ufscar.br/bitstream/ufscar/15059/5/DISSERTA%c3%87%c3%83O-Victoria-2021%20V5-pdf.pdf.jpg75d8db902fbcecaa1aab0a0913dceb6bMD55Carta comprovante homologacao- Victoria Peña.pdf.jpgCarta comprovante homologacao- Victoria Peña.pdf.jpgIM Thumbnailimage/jpeg11874https://repositorio.ufscar.br/bitstream/ufscar/15059/7/Carta%20comprovante%20homologacao-%20Victoria%20Pe%c3%b1a.pdf.jpg061acd0a295c95a8e140f60d9930a720MD57ufscar/150592023-09-18 18:32:19.592oai:repositorio.ufscar.br:ufscar/15059Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:19Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Comparação do perfil de metabólitos secundários de cultivares de soja |
dc.title.alternative.eng.fl_str_mv |
Comparison of the secondary metabolites profile of soybean cultivars |
title |
Comparação do perfil de metabólitos secundários de cultivares de soja |
spellingShingle |
Comparação do perfil de metabólitos secundários de cultivares de soja Arroyo, Victoria Patricia Pena Soja Domesticação Perfil químico Flavonoides Soybean Domestication Chemical profile Flavonoids CIENCIAS EXATAS E DA TERRA::QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::EVOLUCAO, SISTEMATICA E ECOLOGIA QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
title_short |
Comparação do perfil de metabólitos secundários de cultivares de soja |
title_full |
Comparação do perfil de metabólitos secundários de cultivares de soja |
title_fullStr |
Comparação do perfil de metabólitos secundários de cultivares de soja |
title_full_unstemmed |
Comparação do perfil de metabólitos secundários de cultivares de soja |
title_sort |
Comparação do perfil de metabólitos secundários de cultivares de soja |
author |
Arroyo, Victoria Patricia Pena |
author_facet |
Arroyo, Victoria Patricia Pena |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/9409311086029770 |
dc.contributor.author.fl_str_mv |
Arroyo, Victoria Patricia Pena |
dc.contributor.advisor1.fl_str_mv |
Wouters, Felipe Christoff |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4392349445398645 |
dc.contributor.authorID.fl_str_mv |
c87def44-798b-48d0-a83a-7a1c3e81447e |
contributor_str_mv |
Wouters, Felipe Christoff |
dc.subject.por.fl_str_mv |
Soja Domesticação Perfil químico Flavonoides |
topic |
Soja Domesticação Perfil químico Flavonoides Soybean Domestication Chemical profile Flavonoids CIENCIAS EXATAS E DA TERRA::QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::EVOLUCAO, SISTEMATICA E ECOLOGIA QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
dc.subject.eng.fl_str_mv |
Soybean Domestication Chemical profile Flavonoids |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::EVOLUCAO, SISTEMATICA E ECOLOGIA QUIMICA CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS |
description |
Soybean (Glycine max) is one of the most important crops in the world socioeconomic scenario, especially in Brazil. However, this crop is constantly attacked by different pests. Plant resistance against herbivores occurs partially through the production of secondary metabolites such as flavonoids. Wild soybeans and cultivated varieties exhibit different levels of resistance to agricultural pests, which may be a consequence of the variability in their secondary metabolite profiles. Thus, identifying these substances among different genotypes and understanding their role in the insect-plant interaction mechanisms is important for a more comprehensive and reliable characterization of soybean resistance. This work proposes to compare the profile of secondary metabolites between 4 cultivated soybean varieties resistant (PI 229358 and IAC 100) and susceptible (BRS 257 and IAC PL-1) to pests and a wild soybean variety (PI 407270). The chemical profile of young and old leaves in the V3 development stage was analyzed. Plants were grown in a greenhouse and different samples were extracted and analyzed by LC-MS. The analysis of the flavonoid profile in the 5 varieties allowed the identification of 18 isoflavone conjugates (daidzein, genistein and glycitein), some of which were present in greater abundance in old leaves. 39 flavonols were also detected, including kaempferol and quercetin conjugates; accumulated mostly in young leaves. Furthermore, it was shown that wild soybeans have higher levels of flavonoids in their younger leaves, in contrast to cultivated varieties. This information will allow us to understand the dynamics of flavonoids in the insect-plant interaction mechanisms and contribute to the creation of resistant varieties and new targets for the management of agricultural pests, contributing to the sustainable development of agriculture. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-10-28T10:35:01Z |
dc.date.available.fl_str_mv |
2021-10-28T10:35:01Z |
dc.date.issued.fl_str_mv |
2021-07-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
ARROYO, Victoria Patricia Pena. Comparação do perfil de metabólitos secundários de cultivares de soja. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15059. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/15059 |
identifier_str_mv |
ARROYO, Victoria Patricia Pena. Comparação do perfil de metabólitos secundários de cultivares de soja. 2021. Dissertação (Mestrado em Química) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15059. |
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https://repositorio.ufscar.br/handle/ufscar/15059 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Programa de Pós-Graduação em Química - PPGQ |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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