Análise in silico para definição de common core microbiano associado a condições com degradação de surfactante aniônico alquilbenzeno linear sulfonado (LAS)

• Autor(a) principal: Lima, Isadora Soares de
• Data de Publicação: 2023
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFSCAR
• Texto Completo: https://repositorio.ufscar.br/handle/ufscar/17760
• topic: biodegradação; gene 16S RNAr; diversidade biológica; água de lavanderia; biodegradation; biological diversity; laundry water; CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Linear alkylbenzene sulfonate (LAS) is the anionic surfactant of greatest economic interest worldwide. Its biodegradation route involves reactions of fumarate addition, β-oxidation, cleavage of the aromatic ring and desulfonation. Since this is a complex process, the use of varied sets of microorganisms makes it possible to take advantage of different metabolisms and enzymes in the degradation of LAS. However, there is a lack of literature on the microorganisms that play a key role in the degradation of surfactant in biological reactors. This study aimed to define the microbial common core associated with the degradation of anionic surfactant linear alkylbenzene sulfonate in bioreactors, from data mining available in public databases containing raw 16S rRNA gene sequences. Forty-six 16S rRNA gene samples, obtained from 12 scientific papers, were analyzed using bioinformatics tools combined with a workflow in R software. The most abundant genera among the samples were Methanosaeta (4.6%) and C39 (2.3%). The genus SJA-28 was predominant in the common core clusters and was present in six of the seven clusters constructed, followed by the Reyranella and Christensenellaceae R-7 group, found in five common cores, and Candidatus Magasanikbacteria, in four cores. By functional profile analysis, enzymes were found related to the fumarate addition, desulfonation and β-oxidation steps of LAS biodegradation.