Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/7535 |
Resumo: | Schistosomiasis mansoni is a disease caused by Schistosoma mansoni, it affects about 8 million people in Brazil and it is a neglected disease. Moreover, it is necessary to develop new tools for its control, since for over 40 years the same drug has been used to treat it. Thus, the search for vaccines is of great value for the control of schistosomiasis. In this study, we evaluated two recombinant enzymes of S. mansoni, the MTAP and APRT as immunogens, and checked the antiparasitic and anti-inflammatory effects in the schistosomiasis murine model. By means of prior immunization with MTAP and/or APRT, we analyzed, after the infection with S. mansoni, the host response to parasite board determination, immunological cytokines and histopathological changes in the liver of these animals. Our data showed that the MTAP enzyme was able to decrease the number of adult worms in previously immunized animals; APRT enzyme and/or the mix of the two enzymes induced reduction of inflammation in the liver of animals and promoted collagen deposition control; and MTAP enzyme and the mix stimulated a humoral immune response with production of IgG1 and IgE antibodies. Hence we suggest that the enzymes have potential for new studies that would seek a future vaccine target for the control of schistosomiasis. |
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Pagiatto, LucianaAnibal, Fernanda de Freitashttp://lattes.cnpq.br/4918261968772806http://lattes.cnpq.br/205049287109350960e34cab-c33e-4398-a576-a80ed46f81752016-09-27T19:56:30Z2016-09-27T19:56:30Z2016-02-25PAGIATTO, Luciana. Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7535.https://repositorio.ufscar.br/handle/ufscar/7535Schistosomiasis mansoni is a disease caused by Schistosoma mansoni, it affects about 8 million people in Brazil and it is a neglected disease. Moreover, it is necessary to develop new tools for its control, since for over 40 years the same drug has been used to treat it. Thus, the search for vaccines is of great value for the control of schistosomiasis. In this study, we evaluated two recombinant enzymes of S. mansoni, the MTAP and APRT as immunogens, and checked the antiparasitic and anti-inflammatory effects in the schistosomiasis murine model. By means of prior immunization with MTAP and/or APRT, we analyzed, after the infection with S. mansoni, the host response to parasite board determination, immunological cytokines and histopathological changes in the liver of these animals. Our data showed that the MTAP enzyme was able to decrease the number of adult worms in previously immunized animals; APRT enzyme and/or the mix of the two enzymes induced reduction of inflammation in the liver of animals and promoted collagen deposition control; and MTAP enzyme and the mix stimulated a humoral immune response with production of IgG1 and IgE antibodies. Hence we suggest that the enzymes have potential for new studies that would seek a future vaccine target for the control of schistosomiasis.A esquistossomose mansônica é uma doença causada pelo agente etiológico Schistosoma mansoni, acomete cerca de 8 milhões de brasileiros, sendo considerada uma doença negligenciada. Assim, faz-se necessário o desenvolvimento de novas ferramentas para o seu controle, visto que há mais de 40 anos usa-se o mesmo fármaco para o seu tratamento. Dessa forma, a busca por vacinas é de grande valia para o controle da esquistossomose mansônica. Neste estudo, avaliamos duas enzimas recombinantes de S. mansoni, a MTAP e a APRT, como imunógenos e verificamos o efeito antiparasitário e anti-inflamatório no modelo murino da esquistossomose mansônica. Por meio da imunização prévia com MTAP e/ou APRT e após a infecção com S. mansoni, analisamos a resposta do hospedeiro com determinação da carga parasitária, as citocinas imunológicas e as alterações histopatológicas no fígado desses animais. Dessa forma, nossos dados mostraram que a enzima MTAP foi capaz diminuir o número de vermes adultos nos animais previamente imunizados. A imunização com a enzima APRT e/ou com o mix das duas enzimas induziu redução do processo inflamatório no fígado dos animais e promoveu controle do depósito de colágeno. E durante a avaliação da resposta humoral a imunização com a enzima MTAP e o mix estimulou uma resposta específica com produção de anticorpos IgG1 e IgE. Assim, sugerimos que, as enzimas apresentam potencial para novos estudos que buscam um futuro alvo vacinal para o controle da esquistossomose mansônica.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvUFSCarSchistosoma mansoniMTAPAPRTInfecção e controleSchistosoma mansoniMTAPAPRTInfection and controlCIENCIAS BIOLOGICASModulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisOnline600600d0b619ca-16cf-40f9-9e9b-1792083fa39finfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissLP.pdfDissLP.pdfapplication/pdf10003873https://repositorio.ufscar.br/bitstream/ufscar/7535/1/DissLP.pdf89fb02c36205a1922829c70d0aa75ff4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81957https://repositorio.ufscar.br/bitstream/ufscar/7535/2/license.txtae0398b6f8b235e40ad82cba6c50031dMD52TEXTDissLP.pdf.txtDissLP.pdf.txtExtracted texttext/plain139278https://repositorio.ufscar.br/bitstream/ufscar/7535/3/DissLP.pdf.txt464bfb532135f717a3539fe5524bce86MD53THUMBNAILDissLP.pdf.jpgDissLP.pdf.jpgIM Thumbnailimage/jpeg5595https://repositorio.ufscar.br/bitstream/ufscar/7535/4/DissLP.pdf.jpgea5b4416ef818c4823bec6699ba810a5MD54ufscar/75352023-09-18 18:31:08.714oai:repositorio.ufscar.br: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Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:08Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
title |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
spellingShingle |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni Pagiatto, Luciana Schistosoma mansoni MTAP APRT Infecção e controle Schistosoma mansoni MTAP APRT Infection and control CIENCIAS BIOLOGICAS |
title_short |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
title_full |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
title_fullStr |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
title_full_unstemmed |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
title_sort |
Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni |
author |
Pagiatto, Luciana |
author_facet |
Pagiatto, Luciana |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/2050492871093509 |
dc.contributor.author.fl_str_mv |
Pagiatto, Luciana |
dc.contributor.advisor1.fl_str_mv |
Anibal, Fernanda de Freitas |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4918261968772806 |
dc.contributor.authorID.fl_str_mv |
60e34cab-c33e-4398-a576-a80ed46f8175 |
contributor_str_mv |
Anibal, Fernanda de Freitas |
dc.subject.por.fl_str_mv |
Schistosoma mansoni MTAP APRT Infecção e controle |
topic |
Schistosoma mansoni MTAP APRT Infecção e controle Schistosoma mansoni MTAP APRT Infection and control CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Schistosoma mansoni MTAP APRT Infection and control |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
Schistosomiasis mansoni is a disease caused by Schistosoma mansoni, it affects about 8 million people in Brazil and it is a neglected disease. Moreover, it is necessary to develop new tools for its control, since for over 40 years the same drug has been used to treat it. Thus, the search for vaccines is of great value for the control of schistosomiasis. In this study, we evaluated two recombinant enzymes of S. mansoni, the MTAP and APRT as immunogens, and checked the antiparasitic and anti-inflammatory effects in the schistosomiasis murine model. By means of prior immunization with MTAP and/or APRT, we analyzed, after the infection with S. mansoni, the host response to parasite board determination, immunological cytokines and histopathological changes in the liver of these animals. Our data showed that the MTAP enzyme was able to decrease the number of adult worms in previously immunized animals; APRT enzyme and/or the mix of the two enzymes induced reduction of inflammation in the liver of animals and promoted collagen deposition control; and MTAP enzyme and the mix stimulated a humoral immune response with production of IgG1 and IgE antibodies. Hence we suggest that the enzymes have potential for new studies that would seek a future vaccine target for the control of schistosomiasis. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-09-27T19:56:30Z |
dc.date.available.fl_str_mv |
2016-09-27T19:56:30Z |
dc.date.issued.fl_str_mv |
2016-02-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PAGIATTO, Luciana. Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7535. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/7535 |
identifier_str_mv |
PAGIATTO, Luciana. Modulação da infecção murina pelas enzimas recombinantes MTAP e APRT de Schistosoma mansoni. 2016. Dissertação (Mestrado em Genética Evolutiva e Biologia Molecular) – Universidade Federal de São Carlos, São Carlos, 2016. Disponível em: https://repositorio.ufscar.br/handle/ufscar/7535. |
url |
https://repositorio.ufscar.br/handle/ufscar/7535 |
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por |
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por |
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600 600 |
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d0b619ca-16cf-40f9-9e9b-1792083fa39f |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos Câmpus São Carlos |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv |
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UFSCar |
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Universidade Federal de São Carlos Câmpus São Carlos |
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