Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/1369 |
Resumo: | Dexamethasone is widely used in clinical use due to its potent anti-allergic and antiinflammatory effects, but it has been shown that its chronic use can induce several side effects such as hyperglycemia, hypertension, hypercholesterolemia and muscle atrophy. Muscle atrophy occurs by an imbalance between catabolic and anabolic protein levels. Among catabolic proteins, FOXO3a, MuRF-1 and Atrogin-1 are directly related to muscle atrophy induced by dexamethasone. Furthermore, it is known that some inflammatory proteins (TNF-α and IL-6) also participate in reduction of muscle weight. We have shown that aerobic exercise attenuates some of the side effects of dexamethasone, but nothing is known about interval training (IT) performed before and concomitant dexamethasone treatment. This study investigated if interval training is effective in attenuating muscle atrophy induced by dexamethasone and if the proteins FOXO3a, MuRF-1, Atrogina-1, TNF-αand IL-6 are involved in this response. Rats were distributed into 4 groups: sedentary control (SC), sedentary + Dexa (SD), trained control (TC), and trained + Dexa (TD), and underwent an interval training period (50% and 80% of maximal capacity, 2 and 1 min, respectively, 1h/day, 5 days / week, 70 days) or remained sedentary. Dexamethasone was administered during the last 10 days (0.5mg/kg per day i.p.). The rats were weighed weekly during training and daily during the treatment. The tibialis anterior (TA), soleus (SOL) and flexor hallucis longus (FHL) were collected, weighed and stored for analysis of TNF-α, IL-6, FOXO3a, MuRF-1 and Atrogin-1protein levels using electrophoresis method, Western Blotting. Administration of dexamethasone resulted in a significant decrease in body weight (-17%) followed by reduction in TA (-22%) and FHL (-19%) muscles weight. This reduction in muscle weight involved a significant increase in MuRF-1 protein levels in TA (+27%) and FHL (+18%) muscles, although TNF-α (-37%FHL and -15% SOL) and IL-6 (-26% TA, - 24% FHL and -18% SOL) protein levels were reduced. Interval training was effective in blocking the increase of MuRF-1protein level in TA and FHL muscles, moreover interval training significantly reduced FOXO3a production level in TA muscle, in both groups, TC (-27%) and TD (-32%).This response was followed by an attenuation of TA muscle mass after training. Chronic treatment with dexamethasone, as well as training, did not change Atrogin-1protein level. The results of the present study allow us to conclude that MuRF-1 seems to be involved in TA and FHL muscle atrophy induced by dexamethasone treatment, independent of inflammatory proteins signaling. On the other side, interval training determined TA muscle atrophy attenuation by decreasing MuRF-1 and FOXO3a without changes in TNF-α e IL-6 protein levels. |
id |
SCAR_f0c2820aa4ea3955aec47323d5d64eae |
---|---|
oai_identifier_str |
oai:repositorio.ufscar.br:ufscar/1369 |
network_acronym_str |
SCAR |
network_name_str |
Repositório Institucional da UFSCAR |
repository_id_str |
4322 |
spelling |
Martuscelli, Aline MioCardoso, Sandra Lia do Amaralhttp://lattes.cnpq.br/2030708742766455http://lattes.cnpq.br/35802151190411859e1c3a1c-7cb1-43d9-a50c-ae9d04deee1c2016-06-02T19:23:00Z2014-10-302016-06-02T19:23:00Z2014-03-27MARTUSCELLI, Aline Mio. Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona. 2014. 57 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2014.https://repositorio.ufscar.br/handle/ufscar/1369Dexamethasone is widely used in clinical use due to its potent anti-allergic and antiinflammatory effects, but it has been shown that its chronic use can induce several side effects such as hyperglycemia, hypertension, hypercholesterolemia and muscle atrophy. Muscle atrophy occurs by an imbalance between catabolic and anabolic protein levels. Among catabolic proteins, FOXO3a, MuRF-1 and Atrogin-1 are directly related to muscle atrophy induced by dexamethasone. Furthermore, it is known that some inflammatory proteins (TNF-α and IL-6) also participate in reduction of muscle weight. We have shown that aerobic exercise attenuates some of the side effects of dexamethasone, but nothing is known about interval training (IT) performed before and concomitant dexamethasone treatment. This study investigated if interval training is effective in attenuating muscle atrophy induced by dexamethasone and if the proteins FOXO3a, MuRF-1, Atrogina-1, TNF-αand IL-6 are involved in this response. Rats were distributed into 4 groups: sedentary control (SC), sedentary + Dexa (SD), trained control (TC), and trained + Dexa (TD), and underwent an interval training period (50% and 80% of maximal capacity, 2 and 1 min, respectively, 1h/day, 5 days / week, 70 days) or remained sedentary. Dexamethasone was administered during the last 10 days (0.5mg/kg per day i.p.). The rats were weighed weekly during training and daily during the treatment. The tibialis anterior (TA), soleus (SOL) and flexor hallucis longus (FHL) were collected, weighed and stored for analysis of TNF-α, IL-6, FOXO3a, MuRF-1 and Atrogin-1protein levels using electrophoresis method, Western Blotting. Administration of dexamethasone resulted in a significant decrease in body weight (-17%) followed by reduction in TA (-22%) and FHL (-19%) muscles weight. This reduction in muscle weight involved a significant increase in MuRF-1 protein levels in TA (+27%) and FHL (+18%) muscles, although TNF-α (-37%FHL and -15% SOL) and IL-6 (-26% TA, - 24% FHL and -18% SOL) protein levels were reduced. Interval training was effective in blocking the increase of MuRF-1protein level in TA and FHL muscles, moreover interval training significantly reduced FOXO3a production level in TA muscle, in both groups, TC (-27%) and TD (-32%).This response was followed by an attenuation of TA muscle mass after training. Chronic treatment with dexamethasone, as well as training, did not change Atrogin-1protein level. The results of the present study allow us to conclude that MuRF-1 seems to be involved in TA and FHL muscle atrophy induced by dexamethasone treatment, independent of inflammatory proteins signaling. On the other side, interval training determined TA muscle atrophy attenuation by decreasing MuRF-1 and FOXO3a without changes in TNF-α e IL-6 protein levels.A dexametasona é amplamente utilizada no uso clínico, devido ao seu potente efeito antialérgico e anti-inflamátorio, entretanto o uso crônico pode induzir diversos efeitos deletérios, tais como hiperglicemia, hipertensão, hipercolesterolemia e atrofia muscular. A atrofia muscular se dá por um desbalanço entre proteínas catabólicas e anabólicas. Dentre as proteínas catabólicas, a FOXO3a, MuRF-1 e Atrogina-1 estão diretamente relacionadas com a atrofia muscular induzida pela dexametasona. Além disso, já se sabe que algumas proteínas inflamatórias (TNF-α e IL-6) também participam na redução de peso muscular. Demonstramos recentemente que o exercício físico aeróbio atenua alguns dos efeitos deletérios da dexametasona, mas nada se sabe sobre os benefícios do exercício físico intervalado (TI) realizado antes e concomitantemente ao tratamento com a dexametasona. Portanto o objetivo deste trabalho foi investigar se o treinamento intervalado seria efetivo em prevenir a atrofia muscular induzida pela dexametasona (0,5mg/kg por 10 dias) e se as proteinas FOXO3a, MuRF-1, Atrogina-1, TNF-α e IL-6 estão envolvidas nesta resposta. Foram utilizados 56 ratos Wistar, separados em 4 grupos: sedentário controle (SC), sedentário tratado com dexametasona (SD), treinado controle (TC) e treinado tratado com dexametasona (TD). Após adaptação na esteira, os animais foram submetidos ao protocolo de TI (50% e 80% da capacidade máxima, 2 e 1 minutos, respectivamente, 1h / dia, 8 semanas, 5 dias / semana) ou mantidos como sedentários. O peso corporal (PC) foi verificado semanalmente durante o treinamento e diariamente durante o tratamento. Os músculos tibial anterior (TA), sóleo (SOL) e flexor longo do hálux (FHL) foram coletados, pesados e armazenados para análises de produção proteica de TNF-α, IL-6, FOXO3a, MuRF-1 e Atrogina-1 utilizando o método de eletroforese. O TI aumentou a capacidade física máxima dos animais treinados. A administração da dexametasona determinou diminuição significativa de PC (-17%) acompanhado de redução do peso muscular do TA (-22%) e FHL (-19%). Esta redução de peso muscular envolveu a proteína MuRF-1, que aumentou no TA (+27%) e FHL (+18%), embora tenha ocorrido diminuição significativa da produção proteica de TNF-α (-37% FHL e -15% SOL) e IL-6 (-26% TA, -24% FHL e -18% SOL) no grupo SD. O TI foi efetivo em bloquear o aumento de MuRF-1 nos músculos TA e FHL, ademais diminuiu significativamente a produção proteica de FOXO3a no músculo TA, tanto para o grupo TC (-27%) como TD (-32%), resposta acompanhada de atenuação da redução de peso muscular no TA, mas não no FHL (TD, -15%). O tratamento crônico com dexametasona, assim como o TI, não determinou alteração na Atrogina-1. Os resultados do presente estudo nos permitem concluir que a MuRF-1 parece estar envolvida na atrofia nos músculos TA e FHL induzida por 10 dias de tratamento com dexametasona, independentemente das alterações das proteínas inflamatórias (TNF-α e IL-6). O TI prévio, por sua vez, determinou atenuação da atrofia no TA por promover diminuição significativa na produção proteica de MuRF-1e de FOXO3a, sem alterar a produção proteica de TNF-α e IL-6.Financiadora de Estudos e Projetosapplication/pdfporUniversidade Federal de São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarBRDexametasonaTreinamento intervaladoAtrofia muscularProteínasGlicocorticoidesFOXO3aMuRF-1, Atrogina-1Interval trainingMuscle atrophyGlucocorticoidsCIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasonainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-13a6ad161-a3e6-4e92-abd1-27c664de971cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL6281.pdfapplication/pdf1196634https://repositorio.ufscar.br/bitstream/ufscar/1369/1/6281.pdf422ee8c02db41898a26d5fca8418a486MD51TEXT6281.pdf.txt6281.pdf.txtExtracted texttext/plain0https://repositorio.ufscar.br/bitstream/ufscar/1369/2/6281.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52THUMBNAIL6281.pdf.jpg6281.pdf.jpgIM Thumbnailimage/jpeg6908https://repositorio.ufscar.br/bitstream/ufscar/1369/3/6281.pdf.jpg62b9adfd73a823a4333fcb5b20cd9d0dMD53ufscar/13692023-09-18 18:31:29.066oai:repositorio.ufscar.br:ufscar/1369Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:29Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
title |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
spellingShingle |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona Martuscelli, Aline Mio Dexametasona Treinamento intervalado Atrofia muscular Proteínas Glicocorticoides FOXO3a MuRF-1, Atrogina-1 Interval training Muscle atrophy Glucocorticoids CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
title_full |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
title_fullStr |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
title_full_unstemmed |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
title_sort |
Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona |
author |
Martuscelli, Aline Mio |
author_facet |
Martuscelli, Aline Mio |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/3580215119041185 |
dc.contributor.author.fl_str_mv |
Martuscelli, Aline Mio |
dc.contributor.advisor1.fl_str_mv |
Cardoso, Sandra Lia do Amaral |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2030708742766455 |
dc.contributor.authorID.fl_str_mv |
9e1c3a1c-7cb1-43d9-a50c-ae9d04deee1c |
contributor_str_mv |
Cardoso, Sandra Lia do Amaral |
dc.subject.por.fl_str_mv |
Dexametasona Treinamento intervalado Atrofia muscular Proteínas Glicocorticoides FOXO3a MuRF-1, Atrogina-1 |
topic |
Dexametasona Treinamento intervalado Atrofia muscular Proteínas Glicocorticoides FOXO3a MuRF-1, Atrogina-1 Interval training Muscle atrophy Glucocorticoids CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Interval training Muscle atrophy Glucocorticoids |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
Dexamethasone is widely used in clinical use due to its potent anti-allergic and antiinflammatory effects, but it has been shown that its chronic use can induce several side effects such as hyperglycemia, hypertension, hypercholesterolemia and muscle atrophy. Muscle atrophy occurs by an imbalance between catabolic and anabolic protein levels. Among catabolic proteins, FOXO3a, MuRF-1 and Atrogin-1 are directly related to muscle atrophy induced by dexamethasone. Furthermore, it is known that some inflammatory proteins (TNF-α and IL-6) also participate in reduction of muscle weight. We have shown that aerobic exercise attenuates some of the side effects of dexamethasone, but nothing is known about interval training (IT) performed before and concomitant dexamethasone treatment. This study investigated if interval training is effective in attenuating muscle atrophy induced by dexamethasone and if the proteins FOXO3a, MuRF-1, Atrogina-1, TNF-αand IL-6 are involved in this response. Rats were distributed into 4 groups: sedentary control (SC), sedentary + Dexa (SD), trained control (TC), and trained + Dexa (TD), and underwent an interval training period (50% and 80% of maximal capacity, 2 and 1 min, respectively, 1h/day, 5 days / week, 70 days) or remained sedentary. Dexamethasone was administered during the last 10 days (0.5mg/kg per day i.p.). The rats were weighed weekly during training and daily during the treatment. The tibialis anterior (TA), soleus (SOL) and flexor hallucis longus (FHL) were collected, weighed and stored for analysis of TNF-α, IL-6, FOXO3a, MuRF-1 and Atrogin-1protein levels using electrophoresis method, Western Blotting. Administration of dexamethasone resulted in a significant decrease in body weight (-17%) followed by reduction in TA (-22%) and FHL (-19%) muscles weight. This reduction in muscle weight involved a significant increase in MuRF-1 protein levels in TA (+27%) and FHL (+18%) muscles, although TNF-α (-37%FHL and -15% SOL) and IL-6 (-26% TA, - 24% FHL and -18% SOL) protein levels were reduced. Interval training was effective in blocking the increase of MuRF-1protein level in TA and FHL muscles, moreover interval training significantly reduced FOXO3a production level in TA muscle, in both groups, TC (-27%) and TD (-32%).This response was followed by an attenuation of TA muscle mass after training. Chronic treatment with dexamethasone, as well as training, did not change Atrogin-1protein level. The results of the present study allow us to conclude that MuRF-1 seems to be involved in TA and FHL muscle atrophy induced by dexamethasone treatment, independent of inflammatory proteins signaling. On the other side, interval training determined TA muscle atrophy attenuation by decreasing MuRF-1 and FOXO3a without changes in TNF-α e IL-6 protein levels. |
publishDate |
2014 |
dc.date.available.fl_str_mv |
2014-10-30 2016-06-02T19:23:00Z |
dc.date.issued.fl_str_mv |
2014-03-27 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:23:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTUSCELLI, Aline Mio. Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona. 2014. 57 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/1369 |
identifier_str_mv |
MARTUSCELLI, Aline Mio. Efeitos do exercício intervalado na expressão de proteínas inflamatórias e catabólicas na musculatura esquelética de ratos tratados com dexametasona. 2014. 57 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2014. |
url |
https://repositorio.ufscar.br/handle/ufscar/1369 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.confidence.fl_str_mv |
-1 -1 |
dc.relation.authority.fl_str_mv |
3a6ad161-a3e6-4e92-abd1-27c664de971c |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.publisher.program.fl_str_mv |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF |
dc.publisher.initials.fl_str_mv |
UFSCar |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal de São Carlos |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFSCAR instname:Universidade Federal de São Carlos (UFSCAR) instacron:UFSCAR |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
UFSCAR |
institution |
UFSCAR |
reponame_str |
Repositório Institucional da UFSCAR |
collection |
Repositório Institucional da UFSCAR |
bitstream.url.fl_str_mv |
https://repositorio.ufscar.br/bitstream/ufscar/1369/1/6281.pdf https://repositorio.ufscar.br/bitstream/ufscar/1369/2/6281.pdf.txt https://repositorio.ufscar.br/bitstream/ufscar/1369/3/6281.pdf.jpg |
bitstream.checksum.fl_str_mv |
422ee8c02db41898a26d5fca8418a486 d41d8cd98f00b204e9800998ecf8427e 62b9adfd73a823a4333fcb5b20cd9d0d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
|
_version_ |
1802136251591032832 |