Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Detalhes bibliográficos
Autor(a) principal: Montoya-Serrano, Francisco S.
Data de Publicação: 2022
Outros Autores: Dal Vesco, Lírio L., Pescador, Rosete
Tipo de documento: preprint
Idioma: eng
Título da fonte: SciELO Preprints
Texto Completo: https://preprints.scielo.org/index.php/scielo/preprint/view/3480
Resumo: Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.
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spelling Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic RainforestCriopreservação de sementes de Vriesea reitzii Leme & A.F. Costa bromélia endêmica da Mata AtlânticaBromeliaceaecarboidratosgerminaçãomorfohistológiateor de umidadeBromeliaceaecarbohydrategerminationmoisture contentmorpho-histologyVriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.Vriesea reitzii é uma bromélia endêmica da Mata Atlântica. O objetivo deste trabalho foi avaliar a criopreservação de sementes utilizando o método de imersão direta, coletadas de cápsulas aos 120, 135 e 150 dias após a antese (DAA). O teor de água foi determinado antes da criopreservação. A porcentagem de germinação, o índice de velocidade de germinação (GSI) e os carboidratos solúveis totais foram quantificados após a criopreservação. A maior porcentagem de umidade (17,6%) foi observada aos 120 DAA, enquanto a maior porcentagem de germinação (89,6%) e GSI (17,0) foram observadas aos 150 DAA. Em análises de microscopia ótica e eletrônica de transmissão não foram observados danos celulares ou alterações a nível morfohistológico e ultraestrutural após o processo de criopreservação. A partir desses resultados, as sementes de V. reitzii podem ser classificadas como ortodoxas e a criopreservação (+LN) é uma ferramenta eficiente para a conservação ex situ desta espécie.SciELO PreprintsSciELO PreprintsSciELO Preprints2022-01-11info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/348010.1590/2236-8906-08/2021enghttps://preprints.scielo.org/index.php/scielo/article/view/3480/6352Copyright (c) 2022 Francisco S. Montoya-Serrano, Lírio L. Dal Vesco, Rosete Pescadorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMontoya-Serrano, Francisco S.Dal Vesco, Lírio L.Pescador, Rosetereponame:SciELO Preprintsinstname:SciELOinstacron:SCI2022-01-10T22:22:11Zoai:ops.preprints.scielo.org:preprint/3480Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2022-01-10T22:22:11SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
Criopreservação de sementes de Vriesea reitzii Leme & A.F. Costa bromélia endêmica da Mata Atlântica
title Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
spellingShingle Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
Montoya-Serrano, Francisco S.
Bromeliaceae
carboidratos
germinação
morfohistológia
teor de umidade
Bromeliaceae
carbohydrate
germination
moisture content
morpho-histology
title_short Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
title_full Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
title_fullStr Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
title_full_unstemmed Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
title_sort Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest
author Montoya-Serrano, Francisco S.
author_facet Montoya-Serrano, Francisco S.
Dal Vesco, Lírio L.
Pescador, Rosete
author_role author
author2 Dal Vesco, Lírio L.
Pescador, Rosete
author2_role author
author
dc.contributor.author.fl_str_mv Montoya-Serrano, Francisco S.
Dal Vesco, Lírio L.
Pescador, Rosete
dc.subject.por.fl_str_mv Bromeliaceae
carboidratos
germinação
morfohistológia
teor de umidade
Bromeliaceae
carbohydrate
germination
moisture content
morpho-histology
topic Bromeliaceae
carboidratos
germinação
morfohistológia
teor de umidade
Bromeliaceae
carbohydrate
germination
moisture content
morpho-histology
description Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/preprint
info:eu-repo/semantics/publishedVersion
format preprint
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/3480
10.1590/2236-8906-08/2021
url https://preprints.scielo.org/index.php/scielo/preprint/view/3480
identifier_str_mv 10.1590/2236-8906-08/2021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/3480/6352
dc.rights.driver.fl_str_mv Copyright (c) 2022 Francisco S. Montoya-Serrano, Lírio L. Dal Vesco, Rosete Pescador
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Francisco S. Montoya-Serrano, Lírio L. Dal Vesco, Rosete Pescador
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
dc.source.none.fl_str_mv reponame:SciELO Preprints
instname:SciELO
instacron:SCI
instname_str SciELO
instacron_str SCI
institution SCI
reponame_str SciELO Preprints
collection SciELO Preprints
repository.name.fl_str_mv SciELO Preprints - SciELO
repository.mail.fl_str_mv scielo.submission@scielo.org
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