Biosurfactants in the bioremediation of hydrocarbon-contaminated soils

Detalhes bibliográficos
Autor(a) principal: Ugaz-Hoyos, Jesús
Data de Publicação: 2020
Outros Autores: Vega-Cruz, Hilda, Iglesias-Osores, Sebastian, Carreño-Farfan, Carmen
Tipo de documento: preprint
Idioma: spa
Título da fonte: SciELO Preprints
Texto Completo: https://preprints.scielo.org/index.php/scielo/preprint/view/642
Resumo: Bioremediation of petroleum hydrocarbons is favored by biosurfactants. This research aimed to determine the performance of surfactants produced by Pseudomonas spp. degrading petroleum hydrocarbons. samples of contaminated soil and Pseudomonas spp were collected. degradative were considered when they used oil as a source of carbon and energy. Producing bacteria surfactants were selected by testing dispersion drop in the salinity minimum Davis medium with 1% glycerol as carbon source and with the three bacteria reached the largest diameter in the halo emulsion performance was determined. On the floor with an HTP of 22 900 mg g-1 was obtained 78 isolates of Pseudomonas spp., Including 84.62% used the oil as a source of carbon and energy in 24-96 hours. The 92.42% of these bacteria produced biosurfactant evidenced by halos of light crude oil emulsion, with diameters of 10-30 mm. The concentration of surfactants produced by Pseudomonas spp. was 1.0-1.5 gL-1, with 35% yield (Pseudomonas sp. 2HI), 31% (Pseudomonas sp. 8JU) and 19% (Pseudomonas sp. 4CF). surfactant production by Pseudomonas spp demonstrated. degradative oil, and its potential for remediation of contaminated soils.
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spelling Biosurfactants in the bioremediation of hydrocarbon-contaminated soilsBiosurfactantes en la biorremediación de suelos contaminados por hidrocarburosBiossurfactantes na biorremediação de solos contaminados por hidrocarbonetoshidrocarbonetos de petróleosurfactantePseudomonasbiodegradaçãopetroleum hydrocarbonssurfactantPseudomonasbiodegradationhidrocarburos de petróleosurfactantesPseudomonasbiodegradaciónBioremediation of petroleum hydrocarbons is favored by biosurfactants. This research aimed to determine the performance of surfactants produced by Pseudomonas spp. degrading petroleum hydrocarbons. samples of contaminated soil and Pseudomonas spp were collected. degradative were considered when they used oil as a source of carbon and energy. Producing bacteria surfactants were selected by testing dispersion drop in the salinity minimum Davis medium with 1% glycerol as carbon source and with the three bacteria reached the largest diameter in the halo emulsion performance was determined. On the floor with an HTP of 22 900 mg g-1 was obtained 78 isolates of Pseudomonas spp., Including 84.62% used the oil as a source of carbon and energy in 24-96 hours. The 92.42% of these bacteria produced biosurfactant evidenced by halos of light crude oil emulsion, with diameters of 10-30 mm. The concentration of surfactants produced by Pseudomonas spp. was 1.0-1.5 gL-1, with 35% yield (Pseudomonas sp. 2HI), 31% (Pseudomonas sp. 8JU) and 19% (Pseudomonas sp. 4CF). surfactant production by Pseudomonas spp demonstrated. degradative oil, and its potential for remediation of contaminated soils.La biorremediación de hidrocarburos de petróleo es favorecida por los biosurfactantes. El objetivo de la investigación fue determinar el rendimiento de surfactantes producidos por Pseudomonas spp. degradadoras de hidrocarburos de petróleo. Se colectaron muestras de suelo contaminado y Pseudomonas spp. se consideraron degradadoras cuando utilizaron el petróleo como fuente de carbono y energía. Las bacterias productoras de surfactantes se seleccionaron mediante la prueba de dispersión de gota en el medio mínimo salino de Davis con 1% de glicerol como fuente de carbono y con las tres bacterias que alcanzaron el mayor diámetro en el halo de emulsión se determinó el rendimiento. En el suelo con un HTP de 22 900 mg g-1 se obtuvieron 78 aislados de Pseudomonas spp., entre las que el 84,62% utilizó el petróleo como fuente de carbono y energía en 24-96 horas. El 92,42% de estas bacterias produjo biosurfactante evidenciado por los halos de emulsión de petróleo crudo liviano, con diámetros de 10-30 mm. La concentración de surfactantes producidos por Pseudomonas spp. fue de 1,0-1,5 gL-1, con rendimiento de 35% (Pseudomonas sp. 2HI), 31% (Pseudomonas sp. 8JU) y 19% (Pseudomonas sp. 4CF). Se demostró la producción de surfactantes por Pseudomonas spp. degradadoras petróleo, y su potencial para la remediación de suelos contaminados.A biorremediação de hidrocarbonetos de petróleo é favorecida pelos biossurfactantes. O objetivo da investigação foi determinar o rendimento de surfactantes produzidos por Pseudomonas spp. degradadores de hidrocarbonetos de óleo. Amostras de solo contaminado e Pseudomonas spp. eram considerados degradantes quando usavam o óleo como fonte de carbono e energia. As bactérias produtoras de surfactantes foram selecionadas usando o teste de dispersão em gotas no meio salino mínimo de Davis com 1% de glicerol como fonte de carbono e com as três bactérias que atingiram o maior diâmetro no halo da emulsão, o rendimento foi determinado. No solo com HTP de 22.900 mg g-1, foram obtidos 78 isolados de Pseudomonas spp. Entre os quais 84,62% ​​utilizaram o óleo como fonte de carbono e energia em 24-96 horas. 92,42% dessas bactérias produziram biossurfactante evidenciado por halos leves de emulsão de óleo bruto, com diâmetros de 10 a 30 mm. A concentração de surfactantes produzidos por Pseudomonas spp. foi de 1,0-1,5 gL-1, com um rendimento de 35% (Pseudomonas sp. 2HI), 31% (Pseudomonas sp. 8JU) e 19% (Pseudomonas sp. 4CF). A produção de surfactantes por Pseudomonas spp. degradadores de óleo e seu potencial para a remediação de solos contaminados.SciELO PreprintsSciELO PreprintsSciELO Preprints2020-05-29info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/64210.1590/SciELOPreprints.642spahttps://preprints.scielo.org/index.php/scielo/article/view/642/825Copyright (c) 2020 Jesús Ugaz-Hoyos, Hilda Vega-Cruz, Sebastian Iglesias-Osores, Carmen Carreño-Farfanhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessUgaz-Hoyos, JesúsVega-Cruz, HildaIglesias-Osores, SebastianCarreño-Farfan, Carmen reponame:SciELO Preprintsinstname:SciELOinstacron:SCI2020-05-28T20:40:32Zoai:ops.preprints.scielo.org:preprint/642Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2020-05-28T20:40:32SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
Biosurfactantes en la biorremediación de suelos contaminados por hidrocarburos
Biossurfactantes na biorremediação de solos contaminados por hidrocarbonetos
title Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
spellingShingle Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
Ugaz-Hoyos, Jesús
hidrocarbonetos de petróleo
surfactante
Pseudomonas
biodegradação
petroleum hydrocarbons
surfactant
Pseudomonas
biodegradation
hidrocarburos de petróleo
surfactantes
Pseudomonas
biodegradación
title_short Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
title_full Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
title_fullStr Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
title_full_unstemmed Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
title_sort Biosurfactants in the bioremediation of hydrocarbon-contaminated soils
author Ugaz-Hoyos, Jesús
author_facet Ugaz-Hoyos, Jesús
Vega-Cruz, Hilda
Iglesias-Osores, Sebastian
Carreño-Farfan, Carmen
author_role author
author2 Vega-Cruz, Hilda
Iglesias-Osores, Sebastian
Carreño-Farfan, Carmen
author2_role author
author
author
dc.contributor.author.fl_str_mv Ugaz-Hoyos, Jesús
Vega-Cruz, Hilda
Iglesias-Osores, Sebastian
Carreño-Farfan, Carmen
dc.subject.por.fl_str_mv hidrocarbonetos de petróleo
surfactante
Pseudomonas
biodegradação
petroleum hydrocarbons
surfactant
Pseudomonas
biodegradation
hidrocarburos de petróleo
surfactantes
Pseudomonas
biodegradación
topic hidrocarbonetos de petróleo
surfactante
Pseudomonas
biodegradação
petroleum hydrocarbons
surfactant
Pseudomonas
biodegradation
hidrocarburos de petróleo
surfactantes
Pseudomonas
biodegradación
description Bioremediation of petroleum hydrocarbons is favored by biosurfactants. This research aimed to determine the performance of surfactants produced by Pseudomonas spp. degrading petroleum hydrocarbons. samples of contaminated soil and Pseudomonas spp were collected. degradative were considered when they used oil as a source of carbon and energy. Producing bacteria surfactants were selected by testing dispersion drop in the salinity minimum Davis medium with 1% glycerol as carbon source and with the three bacteria reached the largest diameter in the halo emulsion performance was determined. On the floor with an HTP of 22 900 mg g-1 was obtained 78 isolates of Pseudomonas spp., Including 84.62% used the oil as a source of carbon and energy in 24-96 hours. The 92.42% of these bacteria produced biosurfactant evidenced by halos of light crude oil emulsion, with diameters of 10-30 mm. The concentration of surfactants produced by Pseudomonas spp. was 1.0-1.5 gL-1, with 35% yield (Pseudomonas sp. 2HI), 31% (Pseudomonas sp. 8JU) and 19% (Pseudomonas sp. 4CF). surfactant production by Pseudomonas spp demonstrated. degradative oil, and its potential for remediation of contaminated soils.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/preprint
info:eu-repo/semantics/publishedVersion
format preprint
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/642
10.1590/SciELOPreprints.642
url https://preprints.scielo.org/index.php/scielo/preprint/view/642
identifier_str_mv 10.1590/SciELOPreprints.642
dc.language.iso.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/642/825
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
dc.source.none.fl_str_mv reponame:SciELO Preprints
instname:SciELO
instacron:SCI
instname_str SciELO
instacron_str SCI
institution SCI
reponame_str SciELO Preprints
collection SciELO Preprints
repository.name.fl_str_mv SciELO Preprints - SciELO
repository.mail.fl_str_mv scielo.submission@scielo.org
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