Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | preprint |
Idioma: | eng |
Título da fonte: | SciELO Preprints |
Texto Completo: | https://preprints.scielo.org/index.php/scielo/preprint/view/239 |
Resumo: | Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. |
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SciELO Preprints |
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|
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Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19Covid-19SARS-CoV-2next-generation sequencingmetatranscriptomicsHerein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.SciELO PreprintsSciELO PreprintsSciELO Preprints2020-04-29info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/23910.1590/SciELOPreprints.239enghttps://preprints.scielo.org/index.php/scielo/article/view/239/291Copyright (c) 2020 Gubio S. Campos, Silvia I. Sardi, Melissa B. Falcao, Emilia M. M. A. Belitardo, Danilo J. P. G. Rocha, Carolina A. Rolo, Aline D. Menezes, Carina Pinheiro, Rejane H. Carvalho, João P. P. Almeida, Eric R. G. R. Aguiar, Luis G. C. Pachecohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCampos, GubioSardi, SilviaFalcao, MelissaBelitardo, EmiliaRocha, DaniloRolo, CarolinaMenezes, AlinePinheiro, CarinaCarvalho, RejaneAlmeida, JoãoAguiar, EricPacheco, Luisreponame:SciELO Preprintsinstname:SciELOinstacron:SCI2020-04-28T18:04:52Zoai:ops.preprints.scielo.org:preprint/239Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2020-04-28T18:04:52SciELO Preprints - SciELOfalse |
dc.title.none.fl_str_mv |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
title |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
spellingShingle |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 Campos, Gubio Covid-19 SARS-CoV-2 next-generation sequencing metatranscriptomics |
title_short |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
title_full |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
title_fullStr |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
title_full_unstemmed |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
title_sort |
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
author |
Campos, Gubio |
author_facet |
Campos, Gubio Sardi, Silvia Falcao, Melissa Belitardo, Emilia Rocha, Danilo Rolo, Carolina Menezes, Aline Pinheiro, Carina Carvalho, Rejane Almeida, João Aguiar, Eric Pacheco, Luis |
author_role |
author |
author2 |
Sardi, Silvia Falcao, Melissa Belitardo, Emilia Rocha, Danilo Rolo, Carolina Menezes, Aline Pinheiro, Carina Carvalho, Rejane Almeida, João Aguiar, Eric Pacheco, Luis |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Campos, Gubio Sardi, Silvia Falcao, Melissa Belitardo, Emilia Rocha, Danilo Rolo, Carolina Menezes, Aline Pinheiro, Carina Carvalho, Rejane Almeida, João Aguiar, Eric Pacheco, Luis |
dc.subject.por.fl_str_mv |
Covid-19 SARS-CoV-2 next-generation sequencing metatranscriptomics |
topic |
Covid-19 SARS-CoV-2 next-generation sequencing metatranscriptomics |
description |
Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/preprint info:eu-repo/semantics/publishedVersion |
format |
preprint |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://preprints.scielo.org/index.php/scielo/preprint/view/239 10.1590/SciELOPreprints.239 |
url |
https://preprints.scielo.org/index.php/scielo/preprint/view/239 |
identifier_str_mv |
10.1590/SciELOPreprints.239 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://preprints.scielo.org/index.php/scielo/article/view/239/291 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
SciELO Preprints SciELO Preprints SciELO Preprints |
publisher.none.fl_str_mv |
SciELO Preprints SciELO Preprints SciELO Preprints |
dc.source.none.fl_str_mv |
reponame:SciELO Preprints instname:SciELO instacron:SCI |
instname_str |
SciELO |
instacron_str |
SCI |
institution |
SCI |
reponame_str |
SciELO Preprints |
collection |
SciELO Preprints |
repository.name.fl_str_mv |
SciELO Preprints - SciELO |
repository.mail.fl_str_mv |
scielo.submission@scielo.org |
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1797047817185787904 |