Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021

Detalhes bibliográficos
Autor(a) principal: Guimarães, Nathalia Sernizon
Data de Publicação: 2022
Outros Autores: Costa, Murilo Soares, Machado, Elaine Leandro, Sato, Hugo Itaru, Amaral, Eduarda de Carvalho Maia e, Arivabene, Rafaela Galvão, Lourenço, Karine Lima, Tupinambás, Unaí Tupinambás, Fonseca, Flávio Guimarães da, Takahashi, Ricardo Hiroshi Caldeira, Teixeira, Santuza Maria Ribeiro, Alves, Claudia Regina Lindgren
Tipo de documento: preprint
Idioma: por
Título da fonte: SciELO Preprints
Texto Completo: https://preprints.scielo.org/index.php/scielo/preprint/view/3430
Resumo: Objective: To show the feasibility of using combined nasopharyngeal swab auto-collection and pool testing to detect SARS-CoV-2 in epidemiological surveys. Methods: The study involved a sample of 154 students from the Universidade Federal de Minas Gerais, who performed the self-collection of the nasopharyngeal swab in individual booths without supervision. Molecular testing was performed using the pool testing technique. Results: Obtaining samples lasted about 5 minutes each. Analysis 6 was performed to detect endogenous RNA in 40 samples, and the results indicated that no failures resulted from self-collection. None of the pools detected the presence of viral RNA. The cost of performing the molecular test (RT-PCR) by pool testing with samples obtained by self-collection was about 10 times lower than with the usual methods. Conclusion: The investigated strategies showed to be economically feasible and valid for the research of SARS-CoV-2 in epidemiological surveys.
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spelling Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021Autocollección de swabs nasofaríngeos y pruebas moleculares en pool testing como estrategias para la detección del Coronavirus Síndrome Respiratorio Agudo Severo 2 (SARS-CoV-2): viabilidad en estudiantes de medicina de la Universidade Federal de Minas Gerais, 2021Autocoleta de swab nasofaríngeo e teste molecular em pool testing como estratégias para detecção de coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2): viabilidade em estudantes de medicina da Universidade Federal de Minas Gerais, 2021COVID-19Inquéritos EpidemiológicosPandemiaPool TestingRT-PCRSARS-CoV-2COVID-19Epidemiological InquiriesPandemicPool TestingRT-PCRSARS-CoV-2COVID-19Pool TestingRT-PCRSARS-CoV-2Investigaciones EpidemiológicasPandemiaObjective: To show the feasibility of using combined nasopharyngeal swab auto-collection and pool testing to detect SARS-CoV-2 in epidemiological surveys. Methods: The study involved a sample of 154 students from the Universidade Federal de Minas Gerais, who performed the self-collection of the nasopharyngeal swab in individual booths without supervision. Molecular testing was performed using the pool testing technique. Results: Obtaining samples lasted about 5 minutes each. Analysis 6 was performed to detect endogenous RNA in 40 samples, and the results indicated that no failures resulted from self-collection. None of the pools detected the presence of viral RNA. The cost of performing the molecular test (RT-PCR) by pool testing with samples obtained by self-collection was about 10 times lower than with the usual methods. Conclusion: The investigated strategies showed to be economically feasible and valid for the research of SARS-CoV-2 in epidemiological surveys.Objetivo: Demostrar la viabilidad de utilizar el uso combinado de la autocollección de swabs nasofaríngeos y pool testing para la detección del SARS-CoV2 en encuestas epidemiológicas. Métodos: El estudio involucró a una muestra de 154 estudiantes de la Universidade Federal de Minas Gerais, quienes realizaron la autocolección del hisopo nasofaríngeo en cabinas individuales sin supervision. La prueba molecular se realizó utilizando la técnica de prueba de grupo. Resultados: La obtención de muestras duró unos 5 minutos por persona. Se realizó un análisis para detectar RNA endógeno en 40 muestras y los resultados indicaron que no hubo fallas derivadas de la autocolección. Ninguno de los grupos detectó la presencia de RNA viral. El costo de realizar una prueba molecular (RT-PCR) por pool con muestras obtenidas por auto-recolección fue aproximadamente 10 veces menor que con los métodos habituales. Conclusión: Las estrategias investigadas demonstraram ser económicamente viables y válidas para la investigación del SARS-CoV-2 en encuestas epidemiológicas.Objetivo: Demonstrar a viabilidade da utilização combinada da autocoleta de swab nasofaríngeo e pool testing para detecção do SARS-CoV-2 em inquéritos epidemiológicos. Métodos: O estudo envolveu amostra de 154 estudantes da Universidade Federal de Minas Gerais, que realizaram a autocoleta do swab nasofaríngeo em cabines individuais e sem supervisão. O teste molecular foi realizado utilizando-se a técnica de pool testing. Resultados: A obtenção de amostras durou cerca de 5 minutos por pessoa. Realizou-se análise para detecção de RNA endógeno em 40 amostras e os resultados indicaram que não houve falhas decorrentes da autocoleta. Nenhum dos pools detectou presença de RNA viral. O custo da realização do teste molecular (RT-PCR) por pool testing com amostras obtidas por autocoleta foi cerca de dez vezes menor do que nos métodos habituais. Conclusão: As estratégias investigadas mostraram-se economicamente viáveis e válidas para a pesquisa de SARS-CoV-2 em inquéritos epidemiológicos.SciELO PreprintsSciELO PreprintsSciELO Preprints2022-01-05info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/343010.1590/S1679-49742022000100002porhttps://preprints.scielo.org/index.php/scielo/article/view/3430/6279Copyright (c) 2022 Murilo Soares Costas, Nathalia Sernizon Guimarães, Claudia Regina Lindgren Alves, Eliane Leandro Machado, Hugo Itaru Sato, Eduarda de Carvalho Maia e Amaral, Rafaela Galvão Arivabene, Karine Lima Lourenço, Unaí Tupinambás, Flávio Guimarães da Fonseca, Ricardo Hiroshi Caldeira Takahashi, Santuza Maria Ribeiro Teixeirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGuimarães, Nathalia SernizonCosta, Murilo Soares Machado, Elaine Leandro Sato, Hugo Itaru Amaral, Eduarda de Carvalho Maia e Arivabene, Rafaela Galvão Lourenço, Karine Lima Tupinambás, Unaí TupinambásFonseca, Flávio Guimarães da Takahashi, Ricardo Hiroshi Caldeira Teixeira, Santuza Maria Ribeiro Alves, Claudia Regina Lindgren reponame:SciELO Preprintsinstname:SciELOinstacron:SCI2022-01-05T14:00:28Zoai:ops.preprints.scielo.org:preprint/3430Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2022-01-05T14:00:28SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
Autocollección de swabs nasofaríngeos y pruebas moleculares en pool testing como estrategias para la detección del Coronavirus Síndrome Respiratorio Agudo Severo 2 (SARS-CoV-2): viabilidad en estudiantes de medicina de la Universidade Federal de Minas Gerais, 2021
Autocoleta de swab nasofaríngeo e teste molecular em pool testing como estratégias para detecção de coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2): viabilidade em estudantes de medicina da Universidade Federal de Minas Gerais, 2021
title Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
spellingShingle Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
Guimarães, Nathalia Sernizon
COVID-19
Inquéritos Epidemiológicos
Pandemia
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Epidemiological Inquiries
Pandemic
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Pool Testing
RT-PCR
SARS-CoV-2
Investigaciones Epidemiológicas
Pandemia
title_short Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
title_full Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
title_fullStr Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
title_full_unstemmed Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
title_sort Self-collection of nasopharyngeal swab and molecular test in pool testing as strategies for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, 2021
author Guimarães, Nathalia Sernizon
author_facet Guimarães, Nathalia Sernizon
Costa, Murilo Soares
Machado, Elaine Leandro
Sato, Hugo Itaru
Amaral, Eduarda de Carvalho Maia e
Arivabene, Rafaela Galvão
Lourenço, Karine Lima
Tupinambás, Unaí Tupinambás
Fonseca, Flávio Guimarães da
Takahashi, Ricardo Hiroshi Caldeira
Teixeira, Santuza Maria Ribeiro
Alves, Claudia Regina Lindgren
author_role author
author2 Costa, Murilo Soares
Machado, Elaine Leandro
Sato, Hugo Itaru
Amaral, Eduarda de Carvalho Maia e
Arivabene, Rafaela Galvão
Lourenço, Karine Lima
Tupinambás, Unaí Tupinambás
Fonseca, Flávio Guimarães da
Takahashi, Ricardo Hiroshi Caldeira
Teixeira, Santuza Maria Ribeiro
Alves, Claudia Regina Lindgren
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Nathalia Sernizon
Costa, Murilo Soares
Machado, Elaine Leandro
Sato, Hugo Itaru
Amaral, Eduarda de Carvalho Maia e
Arivabene, Rafaela Galvão
Lourenço, Karine Lima
Tupinambás, Unaí Tupinambás
Fonseca, Flávio Guimarães da
Takahashi, Ricardo Hiroshi Caldeira
Teixeira, Santuza Maria Ribeiro
Alves, Claudia Regina Lindgren
dc.subject.por.fl_str_mv COVID-19
Inquéritos Epidemiológicos
Pandemia
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Epidemiological Inquiries
Pandemic
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Pool Testing
RT-PCR
SARS-CoV-2
Investigaciones Epidemiológicas
Pandemia
topic COVID-19
Inquéritos Epidemiológicos
Pandemia
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Epidemiological Inquiries
Pandemic
Pool Testing
RT-PCR
SARS-CoV-2
COVID-19
Pool Testing
RT-PCR
SARS-CoV-2
Investigaciones Epidemiológicas
Pandemia
description Objective: To show the feasibility of using combined nasopharyngeal swab auto-collection and pool testing to detect SARS-CoV-2 in epidemiological surveys. Methods: The study involved a sample of 154 students from the Universidade Federal de Minas Gerais, who performed the self-collection of the nasopharyngeal swab in individual booths without supervision. Molecular testing was performed using the pool testing technique. Results: Obtaining samples lasted about 5 minutes each. Analysis 6 was performed to detect endogenous RNA in 40 samples, and the results indicated that no failures resulted from self-collection. None of the pools detected the presence of viral RNA. The cost of performing the molecular test (RT-PCR) by pool testing with samples obtained by self-collection was about 10 times lower than with the usual methods. Conclusion: The investigated strategies showed to be economically feasible and valid for the research of SARS-CoV-2 in epidemiological surveys.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/preprint
info:eu-repo/semantics/publishedVersion
format preprint
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/3430
10.1590/S1679-49742022000100002
url https://preprints.scielo.org/index.php/scielo/preprint/view/3430
identifier_str_mv 10.1590/S1679-49742022000100002
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/3430/6279
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
dc.source.none.fl_str_mv reponame:SciELO Preprints
instname:SciELO
instacron:SCI
instname_str SciELO
instacron_str SCI
institution SCI
reponame_str SciELO Preprints
collection SciELO Preprints
repository.name.fl_str_mv SciELO Preprints - SciELO
repository.mail.fl_str_mv scielo.submission@scielo.org
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