PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO

Detalhes bibliográficos
Autor(a) principal: SILVEIRA, JOSÉ RICARDO PFEIFER
Data de Publicação: 2002
Outros Autores: CASTRO, LUIS ANTÔNIO SUITA, COUTO, MERY ELIZABETH OLIVEIRA, MARTINS, OLINDA MARIA, BARNI, VALMOR
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Gaúcha (Online)
Texto Completo: http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314
Resumo: Polyclonal antisera against Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) and P chrysanthemi (Pch) were produced from intact, untreated cel Is. Bacterial suspensions with 6 to 7 x CFU/ml (2 ml), emulsified with an equal volume of complete Freund's adjuvant were used as antigens to immunize rabbits. The antigens were injected four times at weekly intervala. The antisera obtained (1:640 titer) were specific against the antigens used and did not exhibit any reaction toward other phytopathogenic bacteria, vírus and fungi, as well as potato, tomato, and tobacco plant extracts. Only antigens presents in the cellular structures of Pca, Pcc and Pch are identified by the antibodies and that the latex agglutination test can be used with di I utions of the antigens in order of 1:1 and 1:10 of the initial concentration (50% T, 550 nm).
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spelling PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATOPRODUÇÃO DE ANTI-SOROS PARA DIAGNOSE DE PECTOBACTÉRIAS CAUSADORAS DE PODRIDÃO MOLE EM BATATA Pectobacterium, carotovorum, atrosepticum, chrysanthenü , immunology, serology, Solanum tuberosumPectobacterium, carotovorum, atrosepticum, chrysanthenü, imunologia, sorologia, solanum tuberosumPolyclonal antisera against Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) and P chrysanthemi (Pch) were produced from intact, untreated cel Is. Bacterial suspensions with 6 to 7 x CFU/ml (2 ml), emulsified with an equal volume of complete Freund's adjuvant were used as antigens to immunize rabbits. The antigens were injected four times at weekly intervala. The antisera obtained (1:640 titer) were specific against the antigens used and did not exhibit any reaction toward other phytopathogenic bacteria, vírus and fungi, as well as potato, tomato, and tobacco plant extracts. Only antigens presents in the cellular structures of Pca, Pcc and Pch are identified by the antibodies and that the latex agglutination test can be used with di I utions of the antigens in order of 1:1 and 1:10 of the initial concentration (50% T, 550 nm). Foram produzidos anti-soros a partir de células inteiras e não tratadas de Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) e P clzrysanthemi (Pch). Utilizou-se como antígeno . 2 ml de suspensão bacteriana na concentração de 6 a 7 x 10 7 UFC/ml, emulsificada com igual volume de adjuvante completo de Freund, fazendo quatro aplicações em coelhos, com intervalos semanais. Os anti-soros obtidos (título 1:640) foram específicos em relação aos antígenos utilizados e não reagiram com outras bactérias, vírus e fungos fitopatogênicos, bem como, com extratos de plantas de batata, fumo e tomateiro. Concluiu-se que apenas antígenos presentes nas estrutu- ras celulares de Pca, Pcc e Pch são identificados pelos anticorpos produzidos e que o teste de aglutinação em látex pode ser realizado com diluições do antígeno nas proporções de 1:1 e 1:10, partindo-se da concentração padrão (50%T; 550 nm). Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural2002-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314Pesquisa Agropecuária Gaúcha; Vol 8 No 1/2 (2002): PESQ. AGROP. GAÚCHA; 7-14Pesquisa Agropecuária Gaúcha; v. 8 n. 1/2 (2002): PESQ. AGROP. GAÚCHA; 7-142595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314/277SILVEIRA, JOSÉ RICARDO PFEIFER CASTRO, LUIS ANTÔNIO SUITACOUTO, MERY ELIZABETH OLIVEIRA MARTINS, OLINDA MARIA BARNI, VALMOR info:eu-repo/semantics/openAccess2024-04-22T12:27:30Zoai:ojs.revistapag.agricultura.rs.gov.br:article/314Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2024-04-22T12:27:30Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse
dc.title.none.fl_str_mv PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
PRODUÇÃO DE ANTI-SOROS PARA DIAGNOSE DE PECTOBACTÉRIAS CAUSADORAS DE PODRIDÃO MOLE EM BATATA
title PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
spellingShingle PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
SILVEIRA, JOSÉ RICARDO PFEIFER
Pectobacterium, carotovorum, atrosepticum, chrysanthenü , immunology, serology, Solanum tuberosum
Pectobacterium, carotovorum, atrosepticum, chrysanthenü, imunologia, sorologia, solanum tuberosum
title_short PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
title_full PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
title_fullStr PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
title_full_unstemmed PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
title_sort PRODUCTION OF ANTISERA FOR DIAGNOSIS OF SOFT-ROTTING PECTOBACTERIAS IN POTATO
author SILVEIRA, JOSÉ RICARDO PFEIFER
author_facet SILVEIRA, JOSÉ RICARDO PFEIFER
CASTRO, LUIS ANTÔNIO SUITA
COUTO, MERY ELIZABETH OLIVEIRA
MARTINS, OLINDA MARIA
BARNI, VALMOR
author_role author
author2 CASTRO, LUIS ANTÔNIO SUITA
COUTO, MERY ELIZABETH OLIVEIRA
MARTINS, OLINDA MARIA
BARNI, VALMOR
author2_role author
author
author
author
dc.contributor.author.fl_str_mv SILVEIRA, JOSÉ RICARDO PFEIFER
CASTRO, LUIS ANTÔNIO SUITA
COUTO, MERY ELIZABETH OLIVEIRA
MARTINS, OLINDA MARIA
BARNI, VALMOR
dc.subject.por.fl_str_mv Pectobacterium, carotovorum, atrosepticum, chrysanthenü , immunology, serology, Solanum tuberosum
Pectobacterium, carotovorum, atrosepticum, chrysanthenü, imunologia, sorologia, solanum tuberosum
topic Pectobacterium, carotovorum, atrosepticum, chrysanthenü , immunology, serology, Solanum tuberosum
Pectobacterium, carotovorum, atrosepticum, chrysanthenü, imunologia, sorologia, solanum tuberosum
description Polyclonal antisera against Pectobacterium carotovorum subsp. atrosepticum (Pca), P carotovorum subsp. carotovorum (Pcc) and P chrysanthemi (Pch) were produced from intact, untreated cel Is. Bacterial suspensions with 6 to 7 x CFU/ml (2 ml), emulsified with an equal volume of complete Freund's adjuvant were used as antigens to immunize rabbits. The antigens were injected four times at weekly intervala. The antisera obtained (1:640 titer) were specific against the antigens used and did not exhibit any reaction toward other phytopathogenic bacteria, vírus and fungi, as well as potato, tomato, and tobacco plant extracts. Only antigens presents in the cellular structures of Pca, Pcc and Pch are identified by the antibodies and that the latex agglutination test can be used with di I utions of the antigens in order of 1:1 and 1:10 of the initial concentration (50% T, 550 nm).
publishDate 2002
dc.date.none.fl_str_mv 2002-12-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314
url http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/314/277
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
publisher.none.fl_str_mv Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural
dc.source.none.fl_str_mv Pesquisa Agropecuária Gaúcha; Vol 8 No 1/2 (2002): PESQ. AGROP. GAÚCHA; 7-14
Pesquisa Agropecuária Gaúcha; v. 8 n. 1/2 (2002): PESQ. AGROP. GAÚCHA; 7-14
2595-7686
0104-9070
reponame:Pesquisa Agropecuária Gaúcha (Online)
instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron:SEAPDR
instname_str Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
instacron_str SEAPDR
institution SEAPDR
reponame_str Pesquisa Agropecuária Gaúcha (Online)
collection Pesquisa Agropecuária Gaúcha (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul
repository.mail.fl_str_mv pag@agricultura.rs.gov.br||
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