Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Gaúcha (Online) |
Texto Completo: | http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/46 |
Resumo: | Desmodium incanum is a perennial species, native in grassland of Rio Grande do Sul, Brazil and popularly known as "pega-pega". Due its good characteristics as forage, the degradation of the natural fields became an alarming reality. The conservation of these species requires the knowledge of the nutritional requirements and the abiotic conditions that the species is adapted. So, besides in vitro culture as germplasm conservation, it is suggested practices such as acclimatization of plants to increase the survival percentage in ex vitro environment. The plant nutritional status in the period prior to the acclimatization may influences the results of survival and development during this process. To this end, it was tested the influence of nutrition of the seedling during in vitro cultivation on the D. incanum acclimatization through variations in the salt composition of the culture medium. Our results showed that the culture medium which provided the greatest survival rate and better plant growth was MS medium with half the standard concentration of salts. On the other hand, we observed that the iron halving, in culture medium, during in vitro culture was highly detrimental to the acclimatization process of D. incanum. So we could confirm the importance of nutritional status on in vitro culture for acclimatization stage. |
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Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. AcclimatizationConcentração de nutrientes durante o cultivo in vitro é crucial para a aclimatização de Desmodium incanum DC. Grassland. Pampa biome. Acclimatization. Nutritional requirements.Pastagens. Bioma Pampa. Aclimatização. Exigências nutricionais.Desmodium incanum is a perennial species, native in grassland of Rio Grande do Sul, Brazil and popularly known as "pega-pega". Due its good characteristics as forage, the degradation of the natural fields became an alarming reality. The conservation of these species requires the knowledge of the nutritional requirements and the abiotic conditions that the species is adapted. So, besides in vitro culture as germplasm conservation, it is suggested practices such as acclimatization of plants to increase the survival percentage in ex vitro environment. The plant nutritional status in the period prior to the acclimatization may influences the results of survival and development during this process. To this end, it was tested the influence of nutrition of the seedling during in vitro cultivation on the D. incanum acclimatization through variations in the salt composition of the culture medium. Our results showed that the culture medium which provided the greatest survival rate and better plant growth was MS medium with half the standard concentration of salts. On the other hand, we observed that the iron halving, in culture medium, during in vitro culture was highly detrimental to the acclimatization process of D. incanum. So we could confirm the importance of nutritional status on in vitro culture for acclimatization stage.Desmodium incanum é uma espécie perene, nativa em pastagens do Rio Grande do Sul, Brasil, e popularmente conhecida como "pega-pega". Devido suas boas características como a forragem, a degradação dos campos naturais tornou-se uma realidade alarmante. A conservação das espécies nativas requer o conhecimento sobre as exigências nutricionais e demais condições abióticas a que a espécie está adaptada. Assim, além do cultivo in vitro como forma de conservação do germoplasma, práticas como a aclimatização de plantas são interessantes para aumentar as taxas de sobrevivência no ambiente ex vitro. Com o objetivo de observar o quanto o estado nutricional, ao qual as plantas estão expostas no período anterior a aclimatação, afeta os resultados de sobrevivência e desenvolvimento das mesmas durante este período, testou-se a influência do suprimento nutricional de mudas de Desmodium incanum produzidas in vitro durante a aclimatação, através de variações na composição de sais do meio de cultura. Os resultados mostraram que o meio de cultura que proporcionou a maior taxa de crescimento e a sobrevivência das plantas foi meio MS com metade da concentração padrão de sais. Por outro lado, observou-se que a redução para metade do ferro, em meio de cultura, durante o cultivo in vitro foi altamente prejudicial para o processo de aclimatação de Desmodium incanum. Com estas informações pode-se confirmar a importância do estado nutricional das plantas durante o cultivo in vitro para a fase de aclimatação.Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural2018-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/4610.36812/pag.2018241/246-54Pesquisa Agropecuária Gaúcha; Vol 24 No 1/2 (2018): PESQ. AGROP. GAÚCHA; 46-54Pesquisa Agropecuária Gaúcha; v. 24 n. 1/2 (2018): PESQ. AGROP. GAÚCHA; 46-542595-76860104-9070reponame:Pesquisa Agropecuária Gaúcha (Online)instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulinstacron:SEAPDRporhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/46/37Maldaner, Joseila Schwalbert, Raíssa Steffen , Gerusa Pauli Kist Saldanha, Cleber Witt Flores, Rejane Conterato, Ionara Fátima info:eu-repo/semantics/openAccess2021-09-24T21:45:50Zoai:ojs.revistapag.agricultura.rs.gov.br:article/46Revistahttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapagPUBhttp://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/oaipag@agricultura.rs.gov.br||2595-76860104-9070opendoar:2021-09-24T21:45:50Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sulfalse |
dc.title.none.fl_str_mv |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization Concentração de nutrientes durante o cultivo in vitro é crucial para a aclimatização de Desmodium incanum DC. |
title |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
spellingShingle |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization Maldaner, Joseila Grassland. Pampa biome. Acclimatization. Nutritional requirements. Pastagens. Bioma Pampa. Aclimatização. Exigências nutricionais. |
title_short |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
title_full |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
title_fullStr |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
title_full_unstemmed |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
title_sort |
Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization |
author |
Maldaner, Joseila |
author_facet |
Maldaner, Joseila Schwalbert, Raíssa Steffen , Gerusa Pauli Kist Saldanha, Cleber Witt Flores, Rejane Conterato, Ionara Fátima |
author_role |
author |
author2 |
Schwalbert, Raíssa Steffen , Gerusa Pauli Kist Saldanha, Cleber Witt Flores, Rejane Conterato, Ionara Fátima |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Maldaner, Joseila Schwalbert, Raíssa Steffen , Gerusa Pauli Kist Saldanha, Cleber Witt Flores, Rejane Conterato, Ionara Fátima |
dc.subject.por.fl_str_mv |
Grassland. Pampa biome. Acclimatization. Nutritional requirements. Pastagens. Bioma Pampa. Aclimatização. Exigências nutricionais. |
topic |
Grassland. Pampa biome. Acclimatization. Nutritional requirements. Pastagens. Bioma Pampa. Aclimatização. Exigências nutricionais. |
description |
Desmodium incanum is a perennial species, native in grassland of Rio Grande do Sul, Brazil and popularly known as "pega-pega". Due its good characteristics as forage, the degradation of the natural fields became an alarming reality. The conservation of these species requires the knowledge of the nutritional requirements and the abiotic conditions that the species is adapted. So, besides in vitro culture as germplasm conservation, it is suggested practices such as acclimatization of plants to increase the survival percentage in ex vitro environment. The plant nutritional status in the period prior to the acclimatization may influences the results of survival and development during this process. To this end, it was tested the influence of nutrition of the seedling during in vitro cultivation on the D. incanum acclimatization through variations in the salt composition of the culture medium. Our results showed that the culture medium which provided the greatest survival rate and better plant growth was MS medium with half the standard concentration of salts. On the other hand, we observed that the iron halving, in culture medium, during in vitro culture was highly detrimental to the acclimatization process of D. incanum. So we could confirm the importance of nutritional status on in vitro culture for acclimatization stage. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/46 10.36812/pag.2018241/246-54 |
url |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/46 |
identifier_str_mv |
10.36812/pag.2018241/246-54 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://revistapag.agricultura.rs.gov.br/ojs/index.php/revistapag/article/view/46/37 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural |
publisher.none.fl_str_mv |
Departamento de Diagnóstico e Pesquisa Agropecuária. Secretaria de Agricultura, Pecuária e Desenvolvimento Rural |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Gaúcha; Vol 24 No 1/2 (2018): PESQ. AGROP. GAÚCHA; 46-54 Pesquisa Agropecuária Gaúcha; v. 24 n. 1/2 (2018): PESQ. AGROP. GAÚCHA; 46-54 2595-7686 0104-9070 reponame:Pesquisa Agropecuária Gaúcha (Online) instname:Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul instacron:SEAPDR |
instname_str |
Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul |
instacron_str |
SEAPDR |
institution |
SEAPDR |
reponame_str |
Pesquisa Agropecuária Gaúcha (Online) |
collection |
Pesquisa Agropecuária Gaúcha (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Gaúcha (Online) - Secretaria de Agricultura, Pecuária e Desenvolvimento Rural do Rio Grande do Sul |
repository.mail.fl_str_mv |
pag@agricultura.rs.gov.br|| |
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1797068776107147264 |