Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

Detalhes bibliográficos
Autor(a) principal: Barbosa,Maria C.
Data de Publicação: 2005
Outros Autores: Tovar,Francisco J., Bressan-Nascimento,Suzete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical entomology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600007
Resumo: Four species of Tephritoidea, three from genus Anastrepha: A. obliqua (Macquart), A. sororcula Zucchi and A. serpentina (Wiedemann), and one from genus Ceratitis, C. capitata (Wiedemann) were compared based on puparium morphology and application of the Heteroduplex Mobility Assay (HMA). Puparia were characterized for the first time using the spiracular posterior plate morphology. Application of HMA allowed the detection of variability in the D2 domain from 28S rRNA gene in all four species (confirmed by sequencing). This is a fast, simple, sensitive and inexpensive assay that was used for the first time in the analysis of two Tephritoidea genera. The detected variability suggests that the tecnique has great potential for rapid determination of infestations with two or more species in the same host fruit.
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spelling Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)Fruit flyTephritoideapupariumHMAFour species of Tephritoidea, three from genus Anastrepha: A. obliqua (Macquart), A. sororcula Zucchi and A. serpentina (Wiedemann), and one from genus Ceratitis, C. capitata (Wiedemann) were compared based on puparium morphology and application of the Heteroduplex Mobility Assay (HMA). Puparia were characterized for the first time using the spiracular posterior plate morphology. Application of HMA allowed the detection of variability in the D2 domain from 28S rRNA gene in all four species (confirmed by sequencing). This is a fast, simple, sensitive and inexpensive assay that was used for the first time in the analysis of two Tephritoidea genera. The detected variability suggests that the tecnique has great potential for rapid determination of infestations with two or more species in the same host fruit.Sociedade Entomológica do Brasil2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600007Neotropical Entomology v.34 n.6 2005reponame:Neotropical entomology (Online)instname:Sociedade Entomológica do Brasil (SEB)instacron:SEB10.1590/S1519-566X2005000600007info:eu-repo/semantics/openAccessBarbosa,Maria C.Tovar,Francisco J.Bressan-Nascimento,Suzeteeng2006-03-20T00:00:00Zoai:scielo:S1519-566X2005000600007Revistahttp://www.scielo.br/neONGhttps://old.scielo.br/oai/scielo-oai.php||editor@seb.org.br1678-80521519-566Xopendoar:2006-03-20T00:00Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)false
dc.title.none.fl_str_mv Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
title Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
spellingShingle Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
Barbosa,Maria C.
Fruit fly
Tephritoidea
puparium
HMA
title_short Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
title_full Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
title_fullStr Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
title_full_unstemmed Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
title_sort Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
author Barbosa,Maria C.
author_facet Barbosa,Maria C.
Tovar,Francisco J.
Bressan-Nascimento,Suzete
author_role author
author2 Tovar,Francisco J.
Bressan-Nascimento,Suzete
author2_role author
author
dc.contributor.author.fl_str_mv Barbosa,Maria C.
Tovar,Francisco J.
Bressan-Nascimento,Suzete
dc.subject.por.fl_str_mv Fruit fly
Tephritoidea
puparium
HMA
topic Fruit fly
Tephritoidea
puparium
HMA
description Four species of Tephritoidea, three from genus Anastrepha: A. obliqua (Macquart), A. sororcula Zucchi and A. serpentina (Wiedemann), and one from genus Ceratitis, C. capitata (Wiedemann) were compared based on puparium morphology and application of the Heteroduplex Mobility Assay (HMA). Puparia were characterized for the first time using the spiracular posterior plate morphology. Application of HMA allowed the detection of variability in the D2 domain from 28S rRNA gene in all four species (confirmed by sequencing). This is a fast, simple, sensitive and inexpensive assay that was used for the first time in the analysis of two Tephritoidea genera. The detected variability suggests that the tecnique has great potential for rapid determination of infestations with two or more species in the same host fruit.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1519-566X2005000600007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Entomológica do Brasil
publisher.none.fl_str_mv Sociedade Entomológica do Brasil
dc.source.none.fl_str_mv Neotropical Entomology v.34 n.6 2005
reponame:Neotropical entomology (Online)
instname:Sociedade Entomológica do Brasil (SEB)
instacron:SEB
instname_str Sociedade Entomológica do Brasil (SEB)
instacron_str SEB
institution SEB
reponame_str Neotropical entomology (Online)
collection Neotropical entomology (Online)
repository.name.fl_str_mv Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)
repository.mail.fl_str_mv ||editor@seb.org.br
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