Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Neotropical entomology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014 |
Resumo: | In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects should be avoided in DNA extraction procedures. Conversely, the low variation among the RAPD profiles obtained from amplifications of genomic DNA extracted from head, legs and thorax/wings indicates that all these body parts of male bees are equally useful and secure for using in isolation and amplification procedures of total genomic DNA. |
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Neotropical entomology (Online) |
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Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina)ApoideaEuglossiniorchid beeeuglossine beemolecular markerIn the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects should be avoided in DNA extraction procedures. Conversely, the low variation among the RAPD profiles obtained from amplifications of genomic DNA extracted from head, legs and thorax/wings indicates that all these body parts of male bees are equally useful and secure for using in isolation and amplification procedures of total genomic DNA.Sociedade Entomológica do Brasil2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014Neotropical Entomology v.35 n.6 2006reponame:Neotropical entomology (Online)instname:Sociedade Entomológica do Brasil (SEB)instacron:SEB10.1590/S1519-566X2006000600014info:eu-repo/semantics/openAccessPascual,Amália N.T.Suzuki,Karen M.Almeida,Fernanda S.Sodré,Leda M.K.Sofia,Silvia H.eng2007-01-31T00:00:00Zoai:scielo:S1519-566X2006000600014Revistahttp://www.scielo.br/neONGhttps://old.scielo.br/oai/scielo-oai.php||editor@seb.org.br1678-80521519-566Xopendoar:2007-01-31T00:00Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)false |
dc.title.none.fl_str_mv |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
title |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
spellingShingle |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) Pascual,Amália N.T. Apoidea Euglossini orchid bee euglossine bee molecular marker |
title_short |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
title_full |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
title_fullStr |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
title_full_unstemmed |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
title_sort |
Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) |
author |
Pascual,Amália N.T. |
author_facet |
Pascual,Amália N.T. Suzuki,Karen M. Almeida,Fernanda S. Sodré,Leda M.K. Sofia,Silvia H. |
author_role |
author |
author2 |
Suzuki,Karen M. Almeida,Fernanda S. Sodré,Leda M.K. Sofia,Silvia H. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pascual,Amália N.T. Suzuki,Karen M. Almeida,Fernanda S. Sodré,Leda M.K. Sofia,Silvia H. |
dc.subject.por.fl_str_mv |
Apoidea Euglossini orchid bee euglossine bee molecular marker |
topic |
Apoidea Euglossini orchid bee euglossine bee molecular marker |
description |
In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects should be avoided in DNA extraction procedures. Conversely, the low variation among the RAPD profiles obtained from amplifications of genomic DNA extracted from head, legs and thorax/wings indicates that all these body parts of male bees are equally useful and secure for using in isolation and amplification procedures of total genomic DNA. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-566X2006000600014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Entomológica do Brasil |
publisher.none.fl_str_mv |
Sociedade Entomológica do Brasil |
dc.source.none.fl_str_mv |
Neotropical Entomology v.35 n.6 2006 reponame:Neotropical entomology (Online) instname:Sociedade Entomológica do Brasil (SEB) instacron:SEB |
instname_str |
Sociedade Entomológica do Brasil (SEB) |
instacron_str |
SEB |
institution |
SEB |
reponame_str |
Neotropical entomology (Online) |
collection |
Neotropical entomology (Online) |
repository.name.fl_str_mv |
Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB) |
repository.mail.fl_str_mv |
||editor@seb.org.br |
_version_ |
1754820846431502336 |