Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Neotropical entomology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400016 |
Resumo: | Diatraea saccharalis (Fabricius) has cryptonephridial type Malphigian tubules (MT). This type of MT is characterized by the penetration of the distal part of the MT into the external walls of the rectum, which is usually lined with a perinephral membrane. The MT is divided into three differentiated regions: proximal, middle and distal. In this study, our objective was to compare the nuclear activities of each one of the three regions of the D. saccharalis MT by using a nuclear basophilic technique and critical electrolyte concentration with a toluidine blue stain at pH 4.0. This method allows differentiation of DNA/protein complexes in in situ and in vitro chromatin. MT chromatin structure in D. saccharalis is variable. Fifth instars have a more decondensed chromatin than fourth instars. The distal tubule region was the most decondensed region of the MT. Our data show an elevated genetic activity of the MT in the pre-metamorphosis period. The distal region of the MT has the highest observed activity, which may be associated with the re-absorption of useful components and the excretion of waste materials. |
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Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentrationCytochemistrylarval developmentcryptonephridial systemDiatraea saccharalis (Fabricius) has cryptonephridial type Malphigian tubules (MT). This type of MT is characterized by the penetration of the distal part of the MT into the external walls of the rectum, which is usually lined with a perinephral membrane. The MT is divided into three differentiated regions: proximal, middle and distal. In this study, our objective was to compare the nuclear activities of each one of the three regions of the D. saccharalis MT by using a nuclear basophilic technique and critical electrolyte concentration with a toluidine blue stain at pH 4.0. This method allows differentiation of DNA/protein complexes in in situ and in vitro chromatin. MT chromatin structure in D. saccharalis is variable. Fifth instars have a more decondensed chromatin than fourth instars. The distal tubule region was the most decondensed region of the MT. Our data show an elevated genetic activity of the MT in the pre-metamorphosis period. The distal region of the MT has the highest observed activity, which may be associated with the re-absorption of useful components and the excretion of waste materials.Sociedade Entomológica do Brasil2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400016Neotropical Entomology v.39 n.4 2010reponame:Neotropical entomology (Online)instname:Sociedade Entomológica do Brasil (SEB)instacron:SEB10.1590/S1519-566X2010000400016info:eu-repo/semantics/openAccessFermino,FábioConte,HélioFalco,José R Peng2010-09-14T00:00:00Zoai:scielo:S1519-566X2010000400016Revistahttp://www.scielo.br/neONGhttps://old.scielo.br/oai/scielo-oai.php||editor@seb.org.br1678-80521519-566Xopendoar:2010-09-14T00:00Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB)false |
dc.title.none.fl_str_mv |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
title |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
spellingShingle |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration Fermino,Fábio Cytochemistry larval development cryptonephridial system |
title_short |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
title_full |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
title_fullStr |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
title_full_unstemmed |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
title_sort |
Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration |
author |
Fermino,Fábio |
author_facet |
Fermino,Fábio Conte,Hélio Falco,José R P |
author_role |
author |
author2 |
Conte,Hélio Falco,José R P |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Fermino,Fábio Conte,Hélio Falco,José R P |
dc.subject.por.fl_str_mv |
Cytochemistry larval development cryptonephridial system |
topic |
Cytochemistry larval development cryptonephridial system |
description |
Diatraea saccharalis (Fabricius) has cryptonephridial type Malphigian tubules (MT). This type of MT is characterized by the penetration of the distal part of the MT into the external walls of the rectum, which is usually lined with a perinephral membrane. The MT is divided into three differentiated regions: proximal, middle and distal. In this study, our objective was to compare the nuclear activities of each one of the three regions of the D. saccharalis MT by using a nuclear basophilic technique and critical electrolyte concentration with a toluidine blue stain at pH 4.0. This method allows differentiation of DNA/protein complexes in in situ and in vitro chromatin. MT chromatin structure in D. saccharalis is variable. Fifth instars have a more decondensed chromatin than fourth instars. The distal tubule region was the most decondensed region of the MT. Our data show an elevated genetic activity of the MT in the pre-metamorphosis period. The distal region of the MT has the highest observed activity, which may be associated with the re-absorption of useful components and the excretion of waste materials. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-566X2010000400016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Entomológica do Brasil |
publisher.none.fl_str_mv |
Sociedade Entomológica do Brasil |
dc.source.none.fl_str_mv |
Neotropical Entomology v.39 n.4 2010 reponame:Neotropical entomology (Online) instname:Sociedade Entomológica do Brasil (SEB) instacron:SEB |
instname_str |
Sociedade Entomológica do Brasil (SEB) |
instacron_str |
SEB |
institution |
SEB |
reponame_str |
Neotropical entomology (Online) |
collection |
Neotropical entomology (Online) |
repository.name.fl_str_mv |
Neotropical entomology (Online) - Sociedade Entomológica do Brasil (SEB) |
repository.mail.fl_str_mv |
||editor@seb.org.br |
_version_ |
1754820848958570496 |