Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth

Detalhes bibliográficos
Autor(a) principal: Corte,Viviana Borges
Data de Publicação: 2008
Outros Autores: Borges,Eduardo Euclydes de Lima e, Ventrella,Marília Contin, Leite,Idalina Tereza de Almeida, Braga,Antônio Jorge Tourinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Árvore (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622008000400005
Resumo: The objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing - DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination.
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spelling Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growthReserve materialscarbohydrateslipidsproteinscalcium oxalateThe objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing - DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination.Sociedade de Investigações Florestais2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622008000400005Revista Árvore v.32 n.4 2008reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/S0100-67622008000400005info:eu-repo/semantics/openAccessCorte,Viviana BorgesBorges,Eduardo Euclydes de Lima eVentrella,Marília ContinLeite,Idalina Tereza de AlmeidaBraga,Antônio Jorge Tourinhoeng2008-10-30T00:00:00Zoai:scielo:S0100-67622008000400005Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2008-10-30T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
title Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
spellingShingle Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
Corte,Viviana Borges
Reserve materials
carbohydrates
lipids
proteins
calcium oxalate
title_short Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
title_full Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
title_fullStr Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
title_full_unstemmed Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
title_sort Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth
author Corte,Viviana Borges
author_facet Corte,Viviana Borges
Borges,Eduardo Euclydes de Lima e
Ventrella,Marília Contin
Leite,Idalina Tereza de Almeida
Braga,Antônio Jorge Tourinho
author_role author
author2 Borges,Eduardo Euclydes de Lima e
Ventrella,Marília Contin
Leite,Idalina Tereza de Almeida
Braga,Antônio Jorge Tourinho
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Corte,Viviana Borges
Borges,Eduardo Euclydes de Lima e
Ventrella,Marília Contin
Leite,Idalina Tereza de Almeida
Braga,Antônio Jorge Tourinho
dc.subject.por.fl_str_mv Reserve materials
carbohydrates
lipids
proteins
calcium oxalate
topic Reserve materials
carbohydrates
lipids
proteins
calcium oxalate
description The objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing - DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622008000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622008000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-67622008000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore v.32 n.4 2008
reponame:Revista Árvore (Online)
instname:Universidade Federal de Viçosa (UFV)
instacron:SIF
instname_str Universidade Federal de Viçosa (UFV)
instacron_str SIF
institution SIF
reponame_str Revista Árvore (Online)
collection Revista Árvore (Online)
repository.name.fl_str_mv Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv ||r.arvore@ufv.br
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