EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Árvore (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622017000400213 |
Resumo: | ABSTRACT Eucalyptus spp. residues of paper industry are a potential lignocellulosic raw material for production of second-generation bioethanol as an alternative to conventional production from cereal crops. Studying the behavior at 40 ºC of a commercial cellulase (Sunson), Eucalyptus sawdust saccharification was carried out under two pH conditions. With the aim to evaluate the bioethanol production from Eucalyptus wood, a strategy combining saccharification and Simultaneous Saccharification and Fermentation (SSF) was undertaken at 40 ºC with a thermotolerant Saccharomyces cerevisiae with different substrate and inoculum concentrations, and different nitrogen sources. At last, the process was carried out in optimal conditions with Saccharomyces cerevisiae M522 and SacSV-10. Saccharification produced more free glucose at pH 5, reaching a maximum of 1.5 g/L. Encouraging results were obtained with 500 mg/L of ammonium sulphate as a nitrogen source and 10 % v/v initial inoculum at 106 cfu/mL concentration. Yeast SacSV-10 was not inhibited by phenols present in the culture media using a wood concentration of 10 g/L, but when the solids concentration was increased, the bioprocess yield was compromised. When the process was carried out in optimal conditions the bioethanol production, expressed as the conversion percentage of cellulose to ethanol, was 71.5 % and 73.6 % for M522 and the mutant strain respectively. The studied properties of the mutant strain provide added value to it, which pose new challenges to national companies dedicated to the production and sale of inputs for bioethanol industry. |
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EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1second-generation bioethanolcellulasesSimultaneous Saccharification and FermentationABSTRACT Eucalyptus spp. residues of paper industry are a potential lignocellulosic raw material for production of second-generation bioethanol as an alternative to conventional production from cereal crops. Studying the behavior at 40 ºC of a commercial cellulase (Sunson), Eucalyptus sawdust saccharification was carried out under two pH conditions. With the aim to evaluate the bioethanol production from Eucalyptus wood, a strategy combining saccharification and Simultaneous Saccharification and Fermentation (SSF) was undertaken at 40 ºC with a thermotolerant Saccharomyces cerevisiae with different substrate and inoculum concentrations, and different nitrogen sources. At last, the process was carried out in optimal conditions with Saccharomyces cerevisiae M522 and SacSV-10. Saccharification produced more free glucose at pH 5, reaching a maximum of 1.5 g/L. Encouraging results were obtained with 500 mg/L of ammonium sulphate as a nitrogen source and 10 % v/v initial inoculum at 106 cfu/mL concentration. Yeast SacSV-10 was not inhibited by phenols present in the culture media using a wood concentration of 10 g/L, but when the solids concentration was increased, the bioprocess yield was compromised. When the process was carried out in optimal conditions the bioethanol production, expressed as the conversion percentage of cellulose to ethanol, was 71.5 % and 73.6 % for M522 and the mutant strain respectively. The studied properties of the mutant strain provide added value to it, which pose new challenges to national companies dedicated to the production and sale of inputs for bioethanol industry.Sociedade de Investigações Florestais2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622017000400213Revista Árvore v.41 n.4 2017reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/1806-90882017000400013info:eu-repo/semantics/openAccessVazquez,Sylvia EnidBuxedas,LucianaBonifacino,SilvanaRamirez,Maria BelenLopez,AnaLopretti,Maryeng2018-05-29T00:00:00Zoai:scielo:S0100-67622017000400213Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2018-05-29T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
title |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
spellingShingle |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 Vazquez,Sylvia Enid second-generation bioethanol cellulases Simultaneous Saccharification and Fermentation |
title_short |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
title_full |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
title_fullStr |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
title_full_unstemmed |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
title_sort |
EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1 |
author |
Vazquez,Sylvia Enid |
author_facet |
Vazquez,Sylvia Enid Buxedas,Luciana Bonifacino,Silvana Ramirez,Maria Belen Lopez,Ana Lopretti,Mary |
author_role |
author |
author2 |
Buxedas,Luciana Bonifacino,Silvana Ramirez,Maria Belen Lopez,Ana Lopretti,Mary |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vazquez,Sylvia Enid Buxedas,Luciana Bonifacino,Silvana Ramirez,Maria Belen Lopez,Ana Lopretti,Mary |
dc.subject.por.fl_str_mv |
second-generation bioethanol cellulases Simultaneous Saccharification and Fermentation |
topic |
second-generation bioethanol cellulases Simultaneous Saccharification and Fermentation |
description |
ABSTRACT Eucalyptus spp. residues of paper industry are a potential lignocellulosic raw material for production of second-generation bioethanol as an alternative to conventional production from cereal crops. Studying the behavior at 40 ºC of a commercial cellulase (Sunson), Eucalyptus sawdust saccharification was carried out under two pH conditions. With the aim to evaluate the bioethanol production from Eucalyptus wood, a strategy combining saccharification and Simultaneous Saccharification and Fermentation (SSF) was undertaken at 40 ºC with a thermotolerant Saccharomyces cerevisiae with different substrate and inoculum concentrations, and different nitrogen sources. At last, the process was carried out in optimal conditions with Saccharomyces cerevisiae M522 and SacSV-10. Saccharification produced more free glucose at pH 5, reaching a maximum of 1.5 g/L. Encouraging results were obtained with 500 mg/L of ammonium sulphate as a nitrogen source and 10 % v/v initial inoculum at 106 cfu/mL concentration. Yeast SacSV-10 was not inhibited by phenols present in the culture media using a wood concentration of 10 g/L, but when the solids concentration was increased, the bioprocess yield was compromised. When the process was carried out in optimal conditions the bioethanol production, expressed as the conversion percentage of cellulose to ethanol, was 71.5 % and 73.6 % for M522 and the mutant strain respectively. The studied properties of the mutant strain provide added value to it, which pose new challenges to national companies dedicated to the production and sale of inputs for bioethanol industry. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622017000400213 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622017000400213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-90882017000400013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
dc.source.none.fl_str_mv |
Revista Árvore v.41 n.4 2017 reponame:Revista Árvore (Online) instname:Universidade Federal de Viçosa (UFV) instacron:SIF |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
SIF |
institution |
SIF |
reponame_str |
Revista Árvore (Online) |
collection |
Revista Árvore (Online) |
repository.name.fl_str_mv |
Revista Árvore (Online) - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
||r.arvore@ufv.br |
_version_ |
1750318002579963904 |