Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)

Detalhes bibliográficos
Autor(a) principal: Oliveira,Daria Pimenta de
Data de Publicação: 2006
Outros Autores: Oliveira,Luiz Edson Mota de, Delú Filho,Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Árvore (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622006000500001
Resumo: The objective of this work was to define the optimal conditions for invertase assay, seeking to determine the ideal parameters for the different isoenzymes of leaf and bark tissues in adult rubber trees. Assays of varying pH, sucrose concentration and temperature of the reaction medium were conducted for the two investigated isoenzymes. The results pointed out the existence of two different pH related isoforms for the two analyzed tissues, with an isoenzyme being more active at pH 5,5 and the other at neutral/alkaline pH. Leaf blade isoenzymes presented similar values for substrate concentration, whereas the bark isoenzyme presented maximum values below those previously reported. The assays at different temperatures presented similar values for leaf isoenzymes, though they have differed significantly among the obtained values.
id SIF-1_c819be71aeed2f7f0aea00b41cbfa897
oai_identifier_str oai:scielo:S0100-67622006000500001
network_acronym_str SIF-1
network_name_str Revista Árvore (Online)
repository_id_str
spelling Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)invertase assayisoenzymerubber treesThe objective of this work was to define the optimal conditions for invertase assay, seeking to determine the ideal parameters for the different isoenzymes of leaf and bark tissues in adult rubber trees. Assays of varying pH, sucrose concentration and temperature of the reaction medium were conducted for the two investigated isoenzymes. The results pointed out the existence of two different pH related isoforms for the two analyzed tissues, with an isoenzyme being more active at pH 5,5 and the other at neutral/alkaline pH. Leaf blade isoenzymes presented similar values for substrate concentration, whereas the bark isoenzyme presented maximum values below those previously reported. The assays at different temperatures presented similar values for leaf isoenzymes, though they have differed significantly among the obtained values.Sociedade de Investigações Florestais2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622006000500001Revista Árvore v.30 n.5 2006reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/S0100-67622006000500001info:eu-repo/semantics/openAccessOliveira,Daria Pimenta deOliveira,Luiz Edson Mota deDelú Filho,Nelsoneng2007-02-01T00:00:00Zoai:scielo:S0100-67622006000500001Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2007-02-01T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
title Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
spellingShingle Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
Oliveira,Daria Pimenta de
invertase assay
isoenzyme
rubber trees
title_short Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
title_full Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
title_fullStr Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
title_full_unstemmed Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
title_sort Optimization of invertase assay conditions in rubber tree plants (Hevea brasiliensis Muell. Arg.)
author Oliveira,Daria Pimenta de
author_facet Oliveira,Daria Pimenta de
Oliveira,Luiz Edson Mota de
Delú Filho,Nelson
author_role author
author2 Oliveira,Luiz Edson Mota de
Delú Filho,Nelson
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,Daria Pimenta de
Oliveira,Luiz Edson Mota de
Delú Filho,Nelson
dc.subject.por.fl_str_mv invertase assay
isoenzyme
rubber trees
topic invertase assay
isoenzyme
rubber trees
description The objective of this work was to define the optimal conditions for invertase assay, seeking to determine the ideal parameters for the different isoenzymes of leaf and bark tissues in adult rubber trees. Assays of varying pH, sucrose concentration and temperature of the reaction medium were conducted for the two investigated isoenzymes. The results pointed out the existence of two different pH related isoforms for the two analyzed tissues, with an isoenzyme being more active at pH 5,5 and the other at neutral/alkaline pH. Leaf blade isoenzymes presented similar values for substrate concentration, whereas the bark isoenzyme presented maximum values below those previously reported. The assays at different temperatures presented similar values for leaf isoenzymes, though they have differed significantly among the obtained values.
publishDate 2006
dc.date.none.fl_str_mv 2006-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622006000500001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622006000500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-67622006000500001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore v.30 n.5 2006
reponame:Revista Árvore (Online)
instname:Universidade Federal de Viçosa (UFV)
instacron:SIF
instname_str Universidade Federal de Viçosa (UFV)
instacron_str SIF
institution SIF
reponame_str Revista Árvore (Online)
collection Revista Árvore (Online)
repository.name.fl_str_mv Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv ||r.arvore@ufv.br
_version_ 1750317996940722176

Registros relacionados