Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

Detalhes bibliográficos
Autor(a) principal: Caron,Angelo Luis
Data de Publicação: 2015
Outros Autores: Picanço-Castro,Virgínia, Ansorge,Sven, Covas,Dimas Tadeu, Kamen,Amine, Swiech,Kamilla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923
Resumo: ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
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spelling Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension CulturesFactor VIIIHek293 cellslentiviral vectorspolyethylenimineserum-free suspension culturetransient transfectionABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.Instituto de Tecnologia do Paraná - Tecpar2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923Brazilian Archives of Biology and Technology v.58 n.6 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132015060367info:eu-repo/semantics/openAccessCaron,Angelo LuisPicanço-Castro,VirgíniaAnsorge,SvenCovas,Dimas TadeuKamen,AmineSwiech,Kamillaeng2015-12-01T00:00:00Zoai:scielo:S1516-89132015000600923Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-12-01T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
title Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
spellingShingle Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
Caron,Angelo Luis
Factor VIII
Hek293 cells
lentiviral vectors
polyethylenimine
serum-free suspension culture
transient transfection
title_short Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
title_full Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
title_fullStr Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
title_full_unstemmed Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
title_sort Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
author Caron,Angelo Luis
author_facet Caron,Angelo Luis
Picanço-Castro,Virgínia
Ansorge,Sven
Covas,Dimas Tadeu
Kamen,Amine
Swiech,Kamilla
author_role author
author2 Picanço-Castro,Virgínia
Ansorge,Sven
Covas,Dimas Tadeu
Kamen,Amine
Swiech,Kamilla
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Caron,Angelo Luis
Picanço-Castro,Virgínia
Ansorge,Sven
Covas,Dimas Tadeu
Kamen,Amine
Swiech,Kamilla
dc.subject.por.fl_str_mv Factor VIII
Hek293 cells
lentiviral vectors
polyethylenimine
serum-free suspension culture
transient transfection
topic Factor VIII
Hek293 cells
lentiviral vectors
polyethylenimine
serum-free suspension culture
transient transfection
description ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132015060367
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.58 n.6 2015
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318277263884288

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