Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013 |
Resumo: | Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae. |
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Brazilian Archives of Biology and Technology |
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Genetic transformation of Drosophila willistoni using piggyBac transposon and GFPTransposable elementsinsect transgenesisgerm-line transformationfluorescent protein markermicroinjectionStudies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae.Instituto de Tecnologia do Paraná - Tecpar2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013Brazilian Archives of Biology and Technology v.50 n.1 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000100013info:eu-repo/semantics/openAccessFinokiet,ManuelaGoni,BeatrizLoreto,Élgion Lúcio Silvaeng2007-05-30T00:00:00Zoai:scielo:S1516-89132007000100013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
title |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
spellingShingle |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP Finokiet,Manuela Transposable elements insect transgenesis germ-line transformation fluorescent protein marker microinjection |
title_short |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
title_full |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
title_fullStr |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
title_full_unstemmed |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
title_sort |
Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP |
author |
Finokiet,Manuela |
author_facet |
Finokiet,Manuela Goni,Beatriz Loreto,Élgion Lúcio Silva |
author_role |
author |
author2 |
Goni,Beatriz Loreto,Élgion Lúcio Silva |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Finokiet,Manuela Goni,Beatriz Loreto,Élgion Lúcio Silva |
dc.subject.por.fl_str_mv |
Transposable elements insect transgenesis germ-line transformation fluorescent protein marker microinjection |
topic |
Transposable elements insect transgenesis germ-line transformation fluorescent protein marker microinjection |
description |
Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132007000100013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.50 n.1 2007 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318271460016128 |