Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP

Detalhes bibliográficos
Autor(a) principal: Finokiet,Manuela
Data de Publicação: 2007
Outros Autores: Goni,Beatriz, Loreto,Élgion Lúcio Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013
Resumo: Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae.
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spelling Genetic transformation of Drosophila willistoni using piggyBac transposon and GFPTransposable elementsinsect transgenesisgerm-line transformationfluorescent protein markermicroinjectionStudies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae.Instituto de Tecnologia do Paraná - Tecpar2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013Brazilian Archives of Biology and Technology v.50 n.1 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000100013info:eu-repo/semantics/openAccessFinokiet,ManuelaGoni,BeatrizLoreto,Élgion Lúcio Silvaeng2007-05-30T00:00:00Zoai:scielo:S1516-89132007000100013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
title Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
spellingShingle Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
Finokiet,Manuela
Transposable elements
insect transgenesis
germ-line transformation
fluorescent protein marker
microinjection
title_short Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
title_full Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
title_fullStr Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
title_full_unstemmed Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
title_sort Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
author Finokiet,Manuela
author_facet Finokiet,Manuela
Goni,Beatriz
Loreto,Élgion Lúcio Silva
author_role author
author2 Goni,Beatriz
Loreto,Élgion Lúcio Silva
author2_role author
author
dc.contributor.author.fl_str_mv Finokiet,Manuela
Goni,Beatriz
Loreto,Élgion Lúcio Silva
dc.subject.por.fl_str_mv Transposable elements
insect transgenesis
germ-line transformation
fluorescent protein marker
microinjection
topic Transposable elements
insect transgenesis
germ-line transformation
fluorescent protein marker
microinjection
description Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high levels of EGFP expression during larvae and adult stages in the eye, abdomen, thorax and legs, suggesting a wide expression pattern in this species than reported to other species of Drosophilidae.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000100013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.1 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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