Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313

Detalhes bibliográficos
Autor(a) principal: Palit,Soumen
Data de Publicação: 2001
Outros Autores: Banerjee,Rintu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
Resumo: The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
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spelling Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313Bacillus circulansSolid state fermentationalpha- amylaseExtractionThe extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd washInstituto de Tecnologia do Paraná - Tecpar2001-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015Brazilian Archives of Biology and Technology v.44 n.1 2001reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132001000100015info:eu-repo/semantics/openAccessPalit,SoumenBanerjee,Rintueng2001-10-25T00:00:00Zoai:scielo:S1516-89132001000100015Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2001-10-25T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
title Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
spellingShingle Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
Palit,Soumen
Bacillus circulans
Solid state fermentation
alpha- amylase
Extraction
title_short Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
title_full Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
title_fullStr Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
title_full_unstemmed Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
title_sort Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
author Palit,Soumen
author_facet Palit,Soumen
Banerjee,Rintu
author_role author
author2 Banerjee,Rintu
author2_role author
dc.contributor.author.fl_str_mv Palit,Soumen
Banerjee,Rintu
dc.subject.por.fl_str_mv Bacillus circulans
Solid state fermentation
alpha- amylase
Extraction
topic Bacillus circulans
Solid state fermentation
alpha- amylase
Extraction
description The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
publishDate 2001
dc.date.none.fl_str_mv 2001-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132001000100015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.44 n.1 2001
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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