Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007 |
Resumo: | Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe proceeded very slowly after the loss of the respiration activity. The starvation stress induced an increase of the endogenous respiration rate at the expense of phenol oxidation. |
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Brazilian Archives of Biology and Technology |
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Physiological changes of Candida tropicalis population degrading phenol in fed batch reactorPhenolbiodegradationCandida tropicalisphenol hydroxylasestarvation stressviabilityCandida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe proceeded very slowly after the loss of the respiration activity. The starvation stress induced an increase of the endogenous respiration rate at the expense of phenol oxidation.Instituto de Tecnologia do Paraná - Tecpar2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007Brazilian Archives of Biology and Technology v.46 n.4 2003reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132003000400007info:eu-repo/semantics/openAccessKomarkova,EliskaPaca,JanKlapkova,EvaStiborova,MarieSoccol,Carlos RSobotka,Miroslaveng2004-02-10T00:00:00Zoai:scielo:S1516-89132003000400007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-02-10T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
title |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
spellingShingle |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor Komarkova,Eliska Phenol biodegradation Candida tropicalis phenol hydroxylase starvation stress viability |
title_short |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
title_full |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
title_fullStr |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
title_full_unstemmed |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
title_sort |
Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor |
author |
Komarkova,Eliska |
author_facet |
Komarkova,Eliska Paca,Jan Klapkova,Eva Stiborova,Marie Soccol,Carlos R Sobotka,Miroslav |
author_role |
author |
author2 |
Paca,Jan Klapkova,Eva Stiborova,Marie Soccol,Carlos R Sobotka,Miroslav |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Komarkova,Eliska Paca,Jan Klapkova,Eva Stiborova,Marie Soccol,Carlos R Sobotka,Miroslav |
dc.subject.por.fl_str_mv |
Phenol biodegradation Candida tropicalis phenol hydroxylase starvation stress viability |
topic |
Phenol biodegradation Candida tropicalis phenol hydroxylase starvation stress viability |
description |
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe proceeded very slowly after the loss of the respiration activity. The starvation stress induced an increase of the endogenous respiration rate at the expense of phenol oxidation. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132003000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.46 n.4 2003 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269474013184 |