Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414 |
Resumo: | Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination. |
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Brazilian Archives of Biology and Technology |
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Dideoxy single allele-specific PCR - DSASP new method to discrimination allelicDSASP methodgenotypingGastric CancerGastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.Instituto de Tecnologia do Paraná - Tecpar2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414Brazilian Archives of Biology and Technology v.58 n.3 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201500434info:eu-repo/semantics/openAccessLima,Eleonidas MouraLopes,Otávio SérgioSoares,Leonardo FerreiraArruda,Talitta DantasGigek,Carolina OliveiraMelo,Cynthia Germoglio FariasSmith,Marilia Arruda CardosoOliveira,João Ricardo GonçalvesMedeiros,ArnaldoDelatorre,PlínioBurbano,Rommel Rodriguezeng2015-10-08T00:00:00Zoai:scielo:S1516-89132015000300414Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-08T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
title |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
spellingShingle |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic Lima,Eleonidas Moura DSASP method genotyping Gastric Cancer |
title_short |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
title_full |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
title_fullStr |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
title_full_unstemmed |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
title_sort |
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
author |
Lima,Eleonidas Moura |
author_facet |
Lima,Eleonidas Moura Lopes,Otávio Sérgio Soares,Leonardo Ferreira Arruda,Talitta Dantas Gigek,Carolina Oliveira Melo,Cynthia Germoglio Farias Smith,Marilia Arruda Cardoso Oliveira,João Ricardo Gonçalves Medeiros,Arnaldo Delatorre,Plínio Burbano,Rommel Rodriguez |
author_role |
author |
author2 |
Lopes,Otávio Sérgio Soares,Leonardo Ferreira Arruda,Talitta Dantas Gigek,Carolina Oliveira Melo,Cynthia Germoglio Farias Smith,Marilia Arruda Cardoso Oliveira,João Ricardo Gonçalves Medeiros,Arnaldo Delatorre,Plínio Burbano,Rommel Rodriguez |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima,Eleonidas Moura Lopes,Otávio Sérgio Soares,Leonardo Ferreira Arruda,Talitta Dantas Gigek,Carolina Oliveira Melo,Cynthia Germoglio Farias Smith,Marilia Arruda Cardoso Oliveira,João Ricardo Gonçalves Medeiros,Arnaldo Delatorre,Plínio Burbano,Rommel Rodriguez |
dc.subject.por.fl_str_mv |
DSASP method genotyping Gastric Cancer |
topic |
DSASP method genotyping Gastric Cancer |
description |
Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201500434 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.58 n.3 2015 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276904222720 |