Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

Detalhes bibliográficos
Autor(a) principal: Lima,Eleonidas Moura
Data de Publicação: 2015
Outros Autores: Lopes,Otávio Sérgio, Soares,Leonardo Ferreira, Arruda,Talitta Dantas, Gigek,Carolina Oliveira, Melo,Cynthia Germoglio Farias, Smith,Marilia Arruda Cardoso, Oliveira,João Ricardo Gonçalves, Medeiros,Arnaldo, Delatorre,Plínio, Burbano,Rommel Rodriguez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414
Resumo: Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
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spelling Dideoxy single allele-specific PCR - DSASP new method to discrimination allelicDSASP methodgenotypingGastric CancerGastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.Instituto de Tecnologia do Paraná - Tecpar2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414Brazilian Archives of Biology and Technology v.58 n.3 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201500434info:eu-repo/semantics/openAccessLima,Eleonidas MouraLopes,Otávio SérgioSoares,Leonardo FerreiraArruda,Talitta DantasGigek,Carolina OliveiraMelo,Cynthia Germoglio FariasSmith,Marilia Arruda CardosoOliveira,João Ricardo GonçalvesMedeiros,ArnaldoDelatorre,PlínioBurbano,Rommel Rodriguezeng2015-10-08T00:00:00Zoai:scielo:S1516-89132015000300414Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-08T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
title Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
spellingShingle Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
Lima,Eleonidas Moura
DSASP method
genotyping
Gastric Cancer
title_short Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
title_full Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
title_fullStr Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
title_full_unstemmed Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
title_sort Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
author Lima,Eleonidas Moura
author_facet Lima,Eleonidas Moura
Lopes,Otávio Sérgio
Soares,Leonardo Ferreira
Arruda,Talitta Dantas
Gigek,Carolina Oliveira
Melo,Cynthia Germoglio Farias
Smith,Marilia Arruda Cardoso
Oliveira,João Ricardo Gonçalves
Medeiros,Arnaldo
Delatorre,Plínio
Burbano,Rommel Rodriguez
author_role author
author2 Lopes,Otávio Sérgio
Soares,Leonardo Ferreira
Arruda,Talitta Dantas
Gigek,Carolina Oliveira
Melo,Cynthia Germoglio Farias
Smith,Marilia Arruda Cardoso
Oliveira,João Ricardo Gonçalves
Medeiros,Arnaldo
Delatorre,Plínio
Burbano,Rommel Rodriguez
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima,Eleonidas Moura
Lopes,Otávio Sérgio
Soares,Leonardo Ferreira
Arruda,Talitta Dantas
Gigek,Carolina Oliveira
Melo,Cynthia Germoglio Farias
Smith,Marilia Arruda Cardoso
Oliveira,João Ricardo Gonçalves
Medeiros,Arnaldo
Delatorre,Plínio
Burbano,Rommel Rodriguez
dc.subject.por.fl_str_mv DSASP method
genotyping
Gastric Cancer
topic DSASP method
genotyping
Gastric Cancer
description Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-8913201500434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.58 n.3 2015
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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