Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

• Main Author: Ribas,Alessandra Ferreira
• Publication Date: 2006
• Other Authors: Kobayashi,Adilson Kenji, Pereira,Luiz Filipe Protasio, Vieira,Luiz Gonzaga Esteves
• Format: Article
• Language: eng
• Source: Brazilian Archives of Biology and Technology
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100002
• topic: Genetic transformation; coffee; herbicide tolerance; ammonium glufosinate

Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl)-adenine (2-iP). Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.