Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

Detalhes bibliográficos
Autor(a) principal: Moreira,Keila Aparecida
Data de Publicação: 2005
Outros Autores: Diogo,Margarida, Prazeres,Duarte Miguel, Lima Filho,José Luiz de, Porto,Ana Lúcia Figueiredo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
Resumo: This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.
id TECPAR-1_1795ab2be8ce9ee3b7c84b711fd1e7cc
oai_identifier_str oai:scielo:S1516-89132005000400014
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatographyGene therapyhydrophobic interaction chromatographyplasmidpurificationThis paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.Instituto de Tecnologia do Paraná - Tecpar2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014Brazilian Archives of Biology and Technology v.48 n.spe 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000400014info:eu-repo/semantics/openAccessMoreira,Keila AparecidaDiogo,MargaridaPrazeres,Duarte MiguelLima Filho,José Luiz dePorto,Ana Lúcia Figueiredoeng2005-08-15T00:00:00Zoai:scielo:S1516-89132005000400014Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-15T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
title Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
spellingShingle Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
Moreira,Keila Aparecida
Gene therapy
hydrophobic interaction chromatography
plasmid
purification
title_short Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
title_full Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
title_fullStr Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
title_full_unstemmed Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
title_sort Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
author Moreira,Keila Aparecida
author_facet Moreira,Keila Aparecida
Diogo,Margarida
Prazeres,Duarte Miguel
Lima Filho,José Luiz de
Porto,Ana Lúcia Figueiredo
author_role author
author2 Diogo,Margarida
Prazeres,Duarte Miguel
Lima Filho,José Luiz de
Porto,Ana Lúcia Figueiredo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Moreira,Keila Aparecida
Diogo,Margarida
Prazeres,Duarte Miguel
Lima Filho,José Luiz de
Porto,Ana Lúcia Figueiredo
dc.subject.por.fl_str_mv Gene therapy
hydrophobic interaction chromatography
plasmid
purification
topic Gene therapy
hydrophobic interaction chromatography
plasmid
purification
description This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000400014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.spe 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318270305533952

Registros relacionados