Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)

Detalhes bibliográficos
Autor(a) principal: Guo,Lina
Data de Publicação: 2018
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440
Resumo: ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity of AcerOr1 protein by IHC was consistent with the results of qRT- PCR and western blotting. The expression of AcerOr1 in non-olfactory tissues implied that, in addition to olfaction, it may involve in other physiological processes. The localization of AcerOr1 in antennae further suggests that it is involved in the olfactory system. Our research suggest that AcerOr1 may participated in perceive pheromone and odours of floral in nurse and forager bees, and also critical for the behavior of collecting, defending, cleaning between the nurse and the forager worker bees.
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spelling Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)Apis cerana ceranaolfactory receptorAcerOr1expressionlocalizationin vivoABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity of AcerOr1 protein by IHC was consistent with the results of qRT- PCR and western blotting. The expression of AcerOr1 in non-olfactory tissues implied that, in addition to olfaction, it may involve in other physiological processes. The localization of AcerOr1 in antennae further suggests that it is involved in the olfactory system. Our research suggest that AcerOr1 may participated in perceive pheromone and odours of floral in nurse and forager bees, and also critical for the behavior of collecting, defending, cleaning between the nurse and the forager worker bees.Instituto de Tecnologia do Paraná - Tecpar2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440Brazilian Archives of Biology and Technology v.61 2018reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2018180091info:eu-repo/semantics/openAccessGuo,Linaeng2019-09-11T00:00:00Zoai:scielo:S1516-89132018000100440Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2019-09-11T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
title Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
spellingShingle Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
Guo,Lina
Apis cerana cerana
olfactory receptor
AcerOr1
expression
localization
in vivo
title_short Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
title_full Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
title_fullStr Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
title_full_unstemmed Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
title_sort Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
author Guo,Lina
author_facet Guo,Lina
author_role author
dc.contributor.author.fl_str_mv Guo,Lina
dc.subject.por.fl_str_mv Apis cerana cerana
olfactory receptor
AcerOr1
expression
localization
in vivo
topic Apis cerana cerana
olfactory receptor
AcerOr1
expression
localization
in vivo
description ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity of AcerOr1 protein by IHC was consistent with the results of qRT- PCR and western blotting. The expression of AcerOr1 in non-olfactory tissues implied that, in addition to olfaction, it may involve in other physiological processes. The localization of AcerOr1 in antennae further suggests that it is involved in the olfactory system. Our research suggest that AcerOr1 may participated in perceive pheromone and odours of floral in nurse and forager bees, and also critical for the behavior of collecting, defending, cleaning between the nurse and the forager worker bees.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2018180091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.61 2018
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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