Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5

Detalhes bibliográficos
Autor(a) principal: Sooch,Balwinder Singh
Data de Publicação: 2013
Outros Autores: Kauldhar,Baljinder Singh
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500002
Resumo: The aim of the present work was to study the influence of multiple bioprocess parameters for the maximum production of lipase from Pseudomonas sp. BWS-5. The culture reached the stationary phase of growth after 36h of incubation when the maximum lipase production was obtained at flask level. The different media components such as carbon sources, nitrogen sources, trace elements and process parameters such as the pH of the medium, temperature and time of incubation, agitation/stationary conditions, etc. were optimized at flask level and at bioreactor level. The maximum enzyme production of 298 IU/mL was obtained with the use of simple medium with pH 6.5 containing glucose (1 %, w/v), peptone (3 %, w/v) and KCl (0.05 %, w/v) after 30h of incubation at 37°C under agitation (200 rpm) conditions with 0.75 vvm of air supply.
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spelling Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5LipasePseudomonasMedium OptimizationThe aim of the present work was to study the influence of multiple bioprocess parameters for the maximum production of lipase from Pseudomonas sp. BWS-5. The culture reached the stationary phase of growth after 36h of incubation when the maximum lipase production was obtained at flask level. The different media components such as carbon sources, nitrogen sources, trace elements and process parameters such as the pH of the medium, temperature and time of incubation, agitation/stationary conditions, etc. were optimized at flask level and at bioreactor level. The maximum enzyme production of 298 IU/mL was obtained with the use of simple medium with pH 6.5 containing glucose (1 %, w/v), peptone (3 %, w/v) and KCl (0.05 %, w/v) after 30h of incubation at 37°C under agitation (200 rpm) conditions with 0.75 vvm of air supply.Instituto de Tecnologia do Paraná - Tecpar2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500002Brazilian Archives of Biology and Technology v.56 n.5 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000500002info:eu-repo/semantics/openAccessSooch,Balwinder SinghKauldhar,Baljinder Singheng2013-10-31T00:00:00Zoai:scielo:S1516-89132013000500002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-10-31T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
title Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
spellingShingle Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
Sooch,Balwinder Singh
Lipase
Pseudomonas
Medium Optimization
title_short Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
title_full Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
title_fullStr Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
title_full_unstemmed Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
title_sort Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5
author Sooch,Balwinder Singh
author_facet Sooch,Balwinder Singh
Kauldhar,Baljinder Singh
author_role author
author2 Kauldhar,Baljinder Singh
author2_role author
dc.contributor.author.fl_str_mv Sooch,Balwinder Singh
Kauldhar,Baljinder Singh
dc.subject.por.fl_str_mv Lipase
Pseudomonas
Medium Optimization
topic Lipase
Pseudomonas
Medium Optimization
description The aim of the present work was to study the influence of multiple bioprocess parameters for the maximum production of lipase from Pseudomonas sp. BWS-5. The culture reached the stationary phase of growth after 36h of incubation when the maximum lipase production was obtained at flask level. The different media components such as carbon sources, nitrogen sources, trace elements and process parameters such as the pH of the medium, temperature and time of incubation, agitation/stationary conditions, etc. were optimized at flask level and at bioreactor level. The maximum enzyme production of 298 IU/mL was obtained with the use of simple medium with pH 6.5 containing glucose (1 %, w/v), peptone (3 %, w/v) and KCl (0.05 %, w/v) after 30h of incubation at 37°C under agitation (200 rpm) conditions with 0.75 vvm of air supply.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013000500002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.56 n.5 2013
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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