Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Archives of Biology and Technology |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100301 |
Resumo: | Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan. |
| id |
TECPAR-1_1cbfba57d0585afabb52e533da8875f3 |
|---|---|
| oai_identifier_str |
oai:scielo:S1516-89132020000100301 |
| network_acronym_str |
TECPAR-1 |
| network_name_str |
Brazilian Archives of Biology and Technology |
| repository_id_str |
|
| spelling |
Biopolymer Xanthan: A New Adjuvant for DNA VaccinesRecombinant DNA vaccinexanthan gumimmunologic adjuvantAbstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.Instituto de Tecnologia do Paraná - Tecpar2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100301Brazilian Archives of Biology and Technology v.63 2020reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2020190090info:eu-repo/semantics/openAccessSilveira,Marcelle MouraConceição,Fabricio RochedoMendonça,MarceloMoreira,Gustavo Marçal Schmidt GarciaCunha,Carlos Eduardo Pouey daRizzi,CarolineHartwig,Daiane DrawanzMoreira,Angelita da SilveiraVendrusculo,Claire TondoMoreira,Ângela Nuneseng2020-09-16T00:00:00Zoai:scielo:S1516-89132020000100301Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2020-09-16T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
| dc.title.none.fl_str_mv |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| title |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| spellingShingle |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines Silveira,Marcelle Moura Recombinant DNA vaccine xanthan gum immunologic adjuvant |
| title_short |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| title_full |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| title_fullStr |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| title_full_unstemmed |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| title_sort |
Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| author |
Silveira,Marcelle Moura |
| author_facet |
Silveira,Marcelle Moura Conceição,Fabricio Rochedo Mendonça,Marcelo Moreira,Gustavo Marçal Schmidt Garcia Cunha,Carlos Eduardo Pouey da Rizzi,Caroline Hartwig,Daiane Drawanz Moreira,Angelita da Silveira Vendrusculo,Claire Tondo Moreira,Ângela Nunes |
| author_role |
author |
| author2 |
Conceição,Fabricio Rochedo Mendonça,Marcelo Moreira,Gustavo Marçal Schmidt Garcia Cunha,Carlos Eduardo Pouey da Rizzi,Caroline Hartwig,Daiane Drawanz Moreira,Angelita da Silveira Vendrusculo,Claire Tondo Moreira,Ângela Nunes |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silveira,Marcelle Moura Conceição,Fabricio Rochedo Mendonça,Marcelo Moreira,Gustavo Marçal Schmidt Garcia Cunha,Carlos Eduardo Pouey da Rizzi,Caroline Hartwig,Daiane Drawanz Moreira,Angelita da Silveira Vendrusculo,Claire Tondo Moreira,Ângela Nunes |
| dc.subject.por.fl_str_mv |
Recombinant DNA vaccine xanthan gum immunologic adjuvant |
| topic |
Recombinant DNA vaccine xanthan gum immunologic adjuvant |
| description |
Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-01-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100301 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100301 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/1678-4324-2020190090 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
| publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
| dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.63 2020 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
| instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
| instacron_str |
TECPAR |
| institution |
TECPAR |
| reponame_str |
Brazilian Archives of Biology and Technology |
| collection |
Brazilian Archives of Biology and Technology |
| repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
| repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
| _version_ |
1750318279663026176 |