Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

Detalhes bibliográficos
Autor(a) principal: Carvalho,Milene Alves de Figueiredo
Data de Publicação: 2014
Outros Autores: Paiva,Renato, Stein,Vanessa Cristina, Herrera,Raírys Cravo, Porto,Jorge Marcelo Padovani, Vargas,Daiane Peixoto, Alves,Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851
Resumo: This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
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spelling Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild PassionfruitPassifloraceaecallogenesiselectromicroscopyoptic microscopetissue cultureThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.Instituto de Tecnologia do Paraná - Tecpar2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851Brazilian Archives of Biology and Technology v.57 n.6 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201402555info:eu-repo/semantics/openAccessCarvalho,Milene Alves de FigueiredoPaiva,RenatoStein,Vanessa CristinaHerrera,Raírys CravoPorto,Jorge Marcelo PadovaniVargas,Daiane PeixotoAlves,Eduardoeng2015-09-03T00:00:00Zoai:scielo:S1516-89132014000600851Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-09-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
title Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
spellingShingle Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
Carvalho,Milene Alves de Figueiredo
Passifloraceae
callogenesis
electromicroscopy
optic microscope
tissue culture
title_short Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
title_full Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
title_fullStr Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
title_full_unstemmed Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
title_sort Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
author Carvalho,Milene Alves de Figueiredo
author_facet Carvalho,Milene Alves de Figueiredo
Paiva,Renato
Stein,Vanessa Cristina
Herrera,Raírys Cravo
Porto,Jorge Marcelo Padovani
Vargas,Daiane Peixoto
Alves,Eduardo
author_role author
author2 Paiva,Renato
Stein,Vanessa Cristina
Herrera,Raírys Cravo
Porto,Jorge Marcelo Padovani
Vargas,Daiane Peixoto
Alves,Eduardo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,Milene Alves de Figueiredo
Paiva,Renato
Stein,Vanessa Cristina
Herrera,Raírys Cravo
Porto,Jorge Marcelo Padovani
Vargas,Daiane Peixoto
Alves,Eduardo
dc.subject.por.fl_str_mv Passifloraceae
callogenesis
electromicroscopy
optic microscope
tissue culture
topic Passifloraceae
callogenesis
electromicroscopy
optic microscope
tissue culture
description This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-8913201402555
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.57 n.6 2014
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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