Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851 |
Resumo: | This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
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Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild PassionfruitPassifloraceaecallogenesiselectromicroscopyoptic microscopetissue cultureThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.Instituto de Tecnologia do Paraná - Tecpar2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851Brazilian Archives of Biology and Technology v.57 n.6 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201402555info:eu-repo/semantics/openAccessCarvalho,Milene Alves de FigueiredoPaiva,RenatoStein,Vanessa CristinaHerrera,Raírys CravoPorto,Jorge Marcelo PadovaniVargas,Daiane PeixotoAlves,Eduardoeng2015-09-03T00:00:00Zoai:scielo:S1516-89132014000600851Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-09-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
title |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
spellingShingle |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit Carvalho,Milene Alves de Figueiredo Passifloraceae callogenesis electromicroscopy optic microscope tissue culture |
title_short |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
title_full |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
title_fullStr |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
title_full_unstemmed |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
title_sort |
Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
author |
Carvalho,Milene Alves de Figueiredo |
author_facet |
Carvalho,Milene Alves de Figueiredo Paiva,Renato Stein,Vanessa Cristina Herrera,Raírys Cravo Porto,Jorge Marcelo Padovani Vargas,Daiane Peixoto Alves,Eduardo |
author_role |
author |
author2 |
Paiva,Renato Stein,Vanessa Cristina Herrera,Raírys Cravo Porto,Jorge Marcelo Padovani Vargas,Daiane Peixoto Alves,Eduardo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho,Milene Alves de Figueiredo Paiva,Renato Stein,Vanessa Cristina Herrera,Raírys Cravo Porto,Jorge Marcelo Padovani Vargas,Daiane Peixoto Alves,Eduardo |
dc.subject.por.fl_str_mv |
Passifloraceae callogenesis electromicroscopy optic microscope tissue culture |
topic |
Passifloraceae callogenesis electromicroscopy optic microscope tissue culture |
description |
This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600851 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201402555 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.6 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276483743744 |