Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells

Detalhes bibliográficos
Autor(a) principal: Polinarski,Celso Aparecido
Data de Publicação: 2005
Outros Autores: Silva,José Luis da Conceição, Mikami,Liya Regina, Fernandez,Maria Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004
Resumo: A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.
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spelling Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cellsInsect polytene cellsNuclear matrixNuclear haloDNA loopsSalivary glandBradysia hygidaA protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.Instituto de Tecnologia do Paraná - Tecpar2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004Brazilian Archives of Biology and Technology v.48 n.3 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000300004info:eu-repo/semantics/openAccessPolinarski,Celso AparecidoSilva,José Luis da ConceiçãoMikami,Liya ReginaFernandez,Maria Aparecidaeng2005-08-03T00:00:00Zoai:scielo:S1516-89132005000300004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
title Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
spellingShingle Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
Polinarski,Celso Aparecido
Insect polytene cells
Nuclear matrix
Nuclear halo
DNA loops
Salivary gland
Bradysia hygida
title_short Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
title_full Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
title_fullStr Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
title_full_unstemmed Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
title_sort Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
author Polinarski,Celso Aparecido
author_facet Polinarski,Celso Aparecido
Silva,José Luis da Conceição
Mikami,Liya Regina
Fernandez,Maria Aparecida
author_role author
author2 Silva,José Luis da Conceição
Mikami,Liya Regina
Fernandez,Maria Aparecida
author2_role author
author
author
dc.contributor.author.fl_str_mv Polinarski,Celso Aparecido
Silva,José Luis da Conceição
Mikami,Liya Regina
Fernandez,Maria Aparecida
dc.subject.por.fl_str_mv Insect polytene cells
Nuclear matrix
Nuclear halo
DNA loops
Salivary gland
Bradysia hygida
topic Insect polytene cells
Nuclear matrix
Nuclear halo
DNA loops
Salivary gland
Bradysia hygida
description A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.3 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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