A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

Detalhes bibliográficos
Autor(a) principal: Zanella,Janice
Data de Publicação: 2012
Outros Autores: Costa,Sergio Olavo Pinto da, Zacaria,Jucimar, Echeverrigaray,Sergio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001
Resumo: Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
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spelling A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samplesAcanthamoebamonocystic culturemicromanipulationAcanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.Instituto de Tecnologia do Paraná - Tecpar2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001Brazilian Archives of Biology and Technology v.55 n.1 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000100001info:eu-repo/semantics/openAccessZanella,JaniceCosta,Sergio Olavo Pinto daZacaria,JucimarEcheverrigaray,Sergioeng2012-03-29T00:00:00Zoai:scielo:S1516-89132012000100001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-03-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
title A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
spellingShingle A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
Zanella,Janice
Acanthamoeba
monocystic culture
micromanipulation
title_short A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
title_full A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
title_fullStr A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
title_full_unstemmed A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
title_sort A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
author Zanella,Janice
author_facet Zanella,Janice
Costa,Sergio Olavo Pinto da
Zacaria,Jucimar
Echeverrigaray,Sergio
author_role author
author2 Costa,Sergio Olavo Pinto da
Zacaria,Jucimar
Echeverrigaray,Sergio
author2_role author
author
author
dc.contributor.author.fl_str_mv Zanella,Janice
Costa,Sergio Olavo Pinto da
Zacaria,Jucimar
Echeverrigaray,Sergio
dc.subject.por.fl_str_mv Acanthamoeba
monocystic culture
micromanipulation
topic Acanthamoeba
monocystic culture
micromanipulation
description Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132012000100001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.55 n.1 2012
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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