Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)

Detalhes bibliográficos
Autor(a) principal: Koç (Yön),Nazan Deniz
Data de Publicação: 2008
Outros Autores: Aytekin,Yener, Yüce,Rikap
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300010
Resumo: Microscopic ovary features of Zebrafish were analyzed. Samples were fixed in Bouin’s solution, mounted in parafin and cut into 6-7 µm-thick slices which were stained with Hematoxylin and Eosin (HE), methylene blue and using Periodic-acid Schiff method (PAS). The thin sections in ultramicrotome with the thickness of approximately 0,5 - 0,7 µm were examined by using Jeol electron microscope following contrasting with Uranyl Acetate-Lead Citrate. The zebrafish was an extremely dynamic organ in which follicles underwent asynchronous development. The oocytes of Zebrafish ovary were observed in various phases. The oocyte development of zebrafish was divided into four stages (primary growth, cortical alveolus, vitellogenic and mature oocyte). Oocyte diameters were observed to vary between 0.,08 mm and 0.76 mm.
id TECPAR-1_2c76e38066f164924dfd019fd3530255
oai_identifier_str oai:scielo:S1516-89132008000300010
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)ZebrafishgonadoocytedevelopmentMicroscopic ovary features of Zebrafish were analyzed. Samples were fixed in Bouin’s solution, mounted in parafin and cut into 6-7 µm-thick slices which were stained with Hematoxylin and Eosin (HE), methylene blue and using Periodic-acid Schiff method (PAS). The thin sections in ultramicrotome with the thickness of approximately 0,5 - 0,7 µm were examined by using Jeol electron microscope following contrasting with Uranyl Acetate-Lead Citrate. The zebrafish was an extremely dynamic organ in which follicles underwent asynchronous development. The oocytes of Zebrafish ovary were observed in various phases. The oocyte development of zebrafish was divided into four stages (primary growth, cortical alveolus, vitellogenic and mature oocyte). Oocyte diameters were observed to vary between 0.,08 mm and 0.76 mm.Instituto de Tecnologia do Paraná - Tecpar2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300010Brazilian Archives of Biology and Technology v.51 n.3 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000300010info:eu-repo/semantics/openAccessKoç (Yön),Nazan DenizAytekin,YenerYüce,Rikapeng2008-07-16T00:00:00Zoai:scielo:S1516-89132008000300010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-07-16T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
title Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
spellingShingle Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
Koç (Yön),Nazan Deniz
Zebrafish
gonad
oocyte
development
title_short Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
title_full Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
title_fullStr Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
title_full_unstemmed Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
title_sort Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
author Koç (Yön),Nazan Deniz
author_facet Koç (Yön),Nazan Deniz
Aytekin,Yener
Yüce,Rikap
author_role author
author2 Aytekin,Yener
Yüce,Rikap
author2_role author
author
dc.contributor.author.fl_str_mv Koç (Yön),Nazan Deniz
Aytekin,Yener
Yüce,Rikap
dc.subject.por.fl_str_mv Zebrafish
gonad
oocyte
development
topic Zebrafish
gonad
oocyte
development
description Microscopic ovary features of Zebrafish were analyzed. Samples were fixed in Bouin’s solution, mounted in parafin and cut into 6-7 µm-thick slices which were stained with Hematoxylin and Eosin (HE), methylene blue and using Periodic-acid Schiff method (PAS). The thin sections in ultramicrotome with the thickness of approximately 0,5 - 0,7 µm were examined by using Jeol electron microscope following contrasting with Uranyl Acetate-Lead Citrate. The zebrafish was an extremely dynamic organ in which follicles underwent asynchronous development. The oocytes of Zebrafish ovary were observed in various phases. The oocyte development of zebrafish was divided into four stages (primary growth, cortical alveolus, vitellogenic and mature oocyte). Oocyte diameters were observed to vary between 0.,08 mm and 0.76 mm.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000300010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.3 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318272250642432

Registros relacionados