Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

Detalhes bibliográficos
Autor(a) principal: Vieira,Felipe Almeida
Data de Publicação: 2006
Outros Autores: Cunha,Maura da, Klein,Denise Espellet, Carvalho,André de Oliveira, Gomes,Valdirene Moreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700004
Resumo: In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2
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spelling Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)beta-1,3-glucanasePR proteinchitinasessecretory structureIn this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2Instituto de Tecnologia do Paraná - Tecpar2006-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700004Brazilian Archives of Biology and Technology v.49 n.6 2006reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132006000700004info:eu-repo/semantics/openAccessVieira,Felipe AlmeidaCunha,Maura daKlein,Denise EspelletCarvalho,André de OliveiraGomes,Valdirene Moreiraeng2007-02-26T00:00:00Zoai:scielo:S1516-89132006000700004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-02-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
title Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
spellingShingle Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
Vieira,Felipe Almeida
beta-1,3-glucanase
PR protein
chitinases
secretory structure
title_short Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
title_full Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
title_fullStr Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
title_full_unstemmed Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
title_sort Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
author Vieira,Felipe Almeida
author_facet Vieira,Felipe Almeida
Cunha,Maura da
Klein,Denise Espellet
Carvalho,André de Oliveira
Gomes,Valdirene Moreira
author_role author
author2 Cunha,Maura da
Klein,Denise Espellet
Carvalho,André de Oliveira
Gomes,Valdirene Moreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vieira,Felipe Almeida
Cunha,Maura da
Klein,Denise Espellet
Carvalho,André de Oliveira
Gomes,Valdirene Moreira
dc.subject.por.fl_str_mv beta-1,3-glucanase
PR protein
chitinases
secretory structure
topic beta-1,3-glucanase
PR protein
chitinases
secretory structure
description In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2
publishDate 2006
dc.date.none.fl_str_mv 2006-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132006000700004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.49 n.6 2006
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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