Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010 |
Resumo: | The present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the presence of some steroids in the isopropyl alcohol extract of C. setigerus:which are (22E)-stigmasta-4,22-dien-3-one(4.93%), δ4-sitosterol-3-one (stigmast-4-en-3-one) (4.31%), fagarsterol (lupeol) (1.25%) and ethyl iso-allocholate (0.32%). Total soluble sugars and chlorophyll-a were also recorded to be highest. The highest activity was exhibited by the isopropyl alcohol and ethyl acetate extract against P. mirabilis. |
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Brazilian Archives of Biology and Technology |
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Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivityCenchrus grassphotosynthetic pigmentstotal soluble sugarprolineAntibacterial activityMICMBC/MFCThe present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the presence of some steroids in the isopropyl alcohol extract of C. setigerus:which are (22E)-stigmasta-4,22-dien-3-one(4.93%), δ4-sitosterol-3-one (stigmast-4-en-3-one) (4.31%), fagarsterol (lupeol) (1.25%) and ethyl iso-allocholate (0.32%). Total soluble sugars and chlorophyll-a were also recorded to be highest. The highest activity was exhibited by the isopropyl alcohol and ethyl acetate extract against P. mirabilis.Instituto de Tecnologia do Paraná - Tecpar2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010Brazilian Archives of Biology and Technology v.57 n.1 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000100010info:eu-repo/semantics/openAccessSingariya,PremlataKumar,PadmaMourya,Krishan Kumareng2014-02-24T00:00:00Zoai:scielo:S1516-89132014000100010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-02-24T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
title |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
spellingShingle |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity Singariya,Premlata Cenchrus grass photosynthetic pigments total soluble sugar proline Antibacterial activity MIC MBC/MFC |
title_short |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
title_full |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
title_fullStr |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
title_full_unstemmed |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
title_sort |
Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity |
author |
Singariya,Premlata |
author_facet |
Singariya,Premlata Kumar,Padma Mourya,Krishan Kumar |
author_role |
author |
author2 |
Kumar,Padma Mourya,Krishan Kumar |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Singariya,Premlata Kumar,Padma Mourya,Krishan Kumar |
dc.subject.por.fl_str_mv |
Cenchrus grass photosynthetic pigments total soluble sugar proline Antibacterial activity MIC MBC/MFC |
topic |
Cenchrus grass photosynthetic pigments total soluble sugar proline Antibacterial activity MIC MBC/MFC |
description |
The present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the presence of some steroids in the isopropyl alcohol extract of C. setigerus:which are (22E)-stigmasta-4,22-dien-3-one(4.93%), δ4-sitosterol-3-one (stigmast-4-en-3-one) (4.31%), fagarsterol (lupeol) (1.25%) and ethyl iso-allocholate (0.32%). Total soluble sugars and chlorophyll-a were also recorded to be highest. The highest activity was exhibited by the isopropyl alcohol and ethyl acetate extract against P. mirabilis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014000100010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.1 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276059070464 |