Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata

Detalhes bibliográficos
Autor(a) principal: Esmaelizad,Majid
Data de Publicação: 2011
Outros Autores: Niaraki,Saber Jelokhani, Fesharaki,Reza Hashemi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005
Resumo: The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran.
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spelling Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulataTams1Theileria Annulatamerozoite surface antigenphylogeneticThe polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran.Instituto de Tecnologia do Paraná - Tecpar2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005Brazilian Archives of Biology and Technology v.54 n.6 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000600005info:eu-repo/semantics/openAccessEsmaelizad,MajidNiaraki,Saber JelokhaniFesharaki,Reza Hashemieng2011-12-13T00:00:00Zoai:scielo:S1516-89132011000600005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-12-13T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
title Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
spellingShingle Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
Esmaelizad,Majid
Tams1
Theileria Annulata
merozoite surface antigen
phylogenetic
title_short Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
title_full Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
title_fullStr Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
title_full_unstemmed Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
title_sort Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
author Esmaelizad,Majid
author_facet Esmaelizad,Majid
Niaraki,Saber Jelokhani
Fesharaki,Reza Hashemi
author_role author
author2 Niaraki,Saber Jelokhani
Fesharaki,Reza Hashemi
author2_role author
author
dc.contributor.author.fl_str_mv Esmaelizad,Majid
Niaraki,Saber Jelokhani
Fesharaki,Reza Hashemi
dc.subject.por.fl_str_mv Tams1
Theileria Annulata
merozoite surface antigen
phylogenetic
topic Tams1
Theileria Annulata
merozoite surface antigen
phylogenetic
description The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132011000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.54 n.6 2011
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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