Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005 |
Resumo: | The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran. |
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Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulataTams1Theileria Annulatamerozoite surface antigenphylogeneticThe polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran.Instituto de Tecnologia do Paraná - Tecpar2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005Brazilian Archives of Biology and Technology v.54 n.6 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000600005info:eu-repo/semantics/openAccessEsmaelizad,MajidNiaraki,Saber JelokhaniFesharaki,Reza Hashemieng2011-12-13T00:00:00Zoai:scielo:S1516-89132011000600005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-12-13T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
title |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
spellingShingle |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata Esmaelizad,Majid Tams1 Theileria Annulata merozoite surface antigen phylogenetic |
title_short |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
title_full |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
title_fullStr |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
title_full_unstemmed |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
title_sort |
Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata |
author |
Esmaelizad,Majid |
author_facet |
Esmaelizad,Majid Niaraki,Saber Jelokhani Fesharaki,Reza Hashemi |
author_role |
author |
author2 |
Niaraki,Saber Jelokhani Fesharaki,Reza Hashemi |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Esmaelizad,Majid Niaraki,Saber Jelokhani Fesharaki,Reza Hashemi |
dc.subject.por.fl_str_mv |
Tams1 Theileria Annulata merozoite surface antigen phylogenetic |
topic |
Tams1 Theileria Annulata merozoite surface antigen phylogenetic |
description |
The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same isolates of T. annulata which were spread in these areas. The phylogenetic tree constructed based on the sequence alignment of 19 Tams1 coding regions was distinctly divided into five lineages. There might be some unknown tick carrier birds immigrating to the different geographical regions. These birds have an effective role to distribute the T. annulata species in North Africa, Palestine and Iran. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132011000600005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.54 n.6 2011 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318274856353792 |