Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675 |
Resumo: | Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role. |
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Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)Antitumor activityArthronema africanumIn vivoIn vitroC-pycocyaninMyeloid Graffi tumorPure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role.Instituto de Tecnologia do Paraná - Tecpar2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675Brazilian Archives of Biology and Technology v.57 n.5 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014005000018info:eu-repo/semantics/openAccessGardeva,ElenaToshkova,RenetaYossifova,LiliyaMinkova,KaledonaIvanova,NataliaGigova,Lilianaeng2015-10-08T00:00:00Zoai:scielo:S1516-89132014000500675Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-08T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
title |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
spellingShingle |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) Gardeva,Elena Antitumor activity Arthronema africanum In vivo In vitro C-pycocyanin Myeloid Graffi tumor |
title_short |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
title_full |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
title_fullStr |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
title_full_unstemmed |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
title_sort |
Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) |
author |
Gardeva,Elena |
author_facet |
Gardeva,Elena Toshkova,Reneta Yossifova,Liliya Minkova,Kaledona Ivanova,Natalia Gigova,Liliana |
author_role |
author |
author2 |
Toshkova,Reneta Yossifova,Liliya Minkova,Kaledona Ivanova,Natalia Gigova,Liliana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gardeva,Elena Toshkova,Reneta Yossifova,Liliya Minkova,Kaledona Ivanova,Natalia Gigova,Liliana |
dc.subject.por.fl_str_mv |
Antitumor activity Arthronema africanum In vivo In vitro C-pycocyanin Myeloid Graffi tumor |
topic |
Antitumor activity Arthronema africanum In vivo In vitro C-pycocyanin Myeloid Graffi tumor |
description |
Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014005000018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.5 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276449140736 |